Effect of adding an anaerobic fungal culture isolated from a wild blue bull (Boselophus tragocamelus) to rumen fluid from buffaloes onin vitro fibrolytic enzyme activity, fermentation and degradation of tannins and tannin-containing Kachnar tree (Bauhinia variegata) leaves and wheat straw

2005 ◽  
Vol 86 (2) ◽  
pp. 258-270 ◽  
Author(s):  
Shyam S Paul ◽  
Devki N Kamra ◽  
Vadali RB Sastry ◽  
Narottam P Sahu
Keyword(s):  
Enzyme Activity ◽  
Wheat Straw ◽  
Rumen Fluid ◽  
Fungal Culture ◽  
Bauhinia Variegata

scholarly journals Trametes suaveolens as ligninolytic enzyme producer

2013 ◽  
pp. 437-444 ◽  
Author(s):  
Aleksandar Knezevic ◽  
Ivan Milovanovic ◽  
Mirjana Stajic ◽  
Jelena Vukojevic
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Nitrogen Concentration ◽  
Pollution Prevention ◽  
Ligninolytic Enzyme ◽  
Species Selection ◽  
Submerged Cultivation ◽  
Phenol Red

Species of the genus Trametes represent one of the most efficient lignin-degraders which can be attributed to a well developed ligninolytic enzyme system. Current trends are screening of ability of new species to produce these enzymes, as well as the optimization of conditions for their overproduction. Therefore, the aim of the study was to evaluate the potential of T. suaveolens to synthesize laccase and Mn-oxidizing peroxidases during fermentation of the selected plant raw materials. Level of enzyme activities was measured on 7, 10 and 14th day of submersion, as well as the solid-state fermentation of wheat straw and oak sawdust in the presence of NH4NO3 in previously determined optimal nitrogen concentration of 25 mM. The enzyme activity was determined spectrophotometrically using ABTS and phenol red as the substrates. The highest level of laccase activity (1087.1 U/L) was noted after 7 days of wheat straw solid-state fermentation, while during the submerged cultivation the production of the enzyme was not noted. Submerged cultivation in oak sawdust-enriched medium was the optimal for activity of Mn-dependent peroxidase (1767.7 U/L on day 14) and Mn-independent peroxidase (1113.7 U/L on day 7). Introduction of T. suaveolens to produce ligninolytic enzyme represented the base for further study, as well as the determination of relation between enzyme activity and rate of lignin degradation. It could lead to greater possibility of fungal species selection with high delignification capacity, which could take participation in sustainable production of food, feed, fibres, and energy, environmentally friendly pollution prevention, and bioremediation.

Effect of administration of rumen fungi on production performance of lactating buffaloes

2010 ◽  
Vol 1 (2) ◽  
pp. 183-188 ◽  
Author(s):  
S. Saxena ◽  
J. Sehgal ◽  
A. Puniya ◽  
K. Singh
Keyword(s):  
Wheat Straw ◽  
Milk Production ◽  
Milk Fat ◽  
Volatile Fatty Acids ◽  
Production Performance ◽  
Control Group ◽  
Fungal Culture ◽  
Group I ◽  
Lactating Buffaloes ◽  
Group Ii

Anaerobic fungi were orally dosed to lactating buffaloes to study their effect on the digestibility of a diet (composed of 50% wheat straw and 50% concentrate along with six kg maize green/animal/day), rumen fermentation patterns and milk production. Group I (control) was administered with fungus-free anaerobic broth, while group II and III were administered with Orpinomyces sp. C-14 or Piromyces sp. WNG-12 (250 ml; 3-5 days of growth/animal/ week), respectively. Milk production was higher in group II and III (8.42 and 8.48 kg/d) than in the control (8.03 kg/d) with virtually the same feed intake (i.e. 11.50 and 10.62 and 11.79 kg, respectively). There was an increase of 6% fat-corrected milk yield/animal/day in group II and III, respectively compared to the control. The milk fat was higher in the fungal culture administered groups than in the control group. The digestibility of dry matter, crude protein, neutral detergent fibre, acid detergent fibre, cellulose and digestible energy also increased significantly in group II and III. The pH and ammonia nitrogen were lower, whereas total volatile fatty acids, total nitrogen, trichloroacid precipitable nitrogen and number of zoospores/ml of rumen liquor were higher in group II and III when compared to the control. Hence, it can be stated that rumen fungi can be used as a direct-fed microbial in lactating buffaloes, to enhance the digestibility of wheat straw based diets leading to higher production.

scholarly journals A Novel Promising Strain ofTrichoderma evansii(WF-3) for Extracellularα-Galactosidase Production by Utilizing Different Carbon Sources under Optimized Culture Conditions

2014 ◽  
Vol 2014 ◽  
pp. 1-12
Author(s):  
Aishwarya Chauhan ◽  
Nikhat Jamal Siddiqi ◽  
Bechan Sharma
Keyword(s):  
Enzyme Activity ◽  
Wheat Straw ◽  
Guar Gum ◽  
Carbon Sources ◽  
Culture Conditions ◽  
Enzyme Synthesis ◽  
Basic Medium ◽  
Growth Media ◽  
Bean Meal ◽  
Soya Bean Meal

A potential fungal strain ofTrichodermasp. (WF-3) was isolated and selected for the production ofα-galactosidase. Optimum conditions for mycelial growth and enzyme induction were determined. Basal media selected for the growth of fungal isolate containing different carbon sources like guar gum (GG), soya bean meal (SM), and wheat straw (WS) and combinations of these carbon substrates with basic sugars like galactose and sucrose were used to monitor their effects onα-galactosidase production. The results of this study indicated that galactose and sucrose enhanced the enzyme activity in guar gum (GG) and wheat straw (WS). Maximumα-galactosidase production (213.63 UmL−1) was obtained when the basic medium containing GG is supplemented with galactose (5 mg/mL). However, the presence of galactose and sucrose alone in the growth media shows no effect. Soya meal alone was able to supportT. evansiito produce maximum enzyme activity (170.36 UmL−1). The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5–5.5, respectively. All the carbon sources tested exhibited maximum enzyme production at 10 mg/mL concentration. Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme. Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

Profile of isoacids in rumen fluid and influence of added isoacids on in sacco dry matter disappearance of untreated and ammonia treated wheat straw

1988 ◽  
Vol 38 (5) ◽  
pp. 431-439 ◽  
Author(s):  
G. Flachowsky ◽  
D. C. Maria Matthey ◽  
W. I. Ochrimenko ◽  
Dai M. Schneider
Keyword(s):  
Wheat Straw ◽  
Dry Matter ◽  
Rumen Fluid ◽  
In Sacco

scholarly journals Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2527
Author(s):  
Yangyi Hao ◽  
Chunyan Guo ◽  
Yue Gong ◽  
Xiaoge Sun ◽  
Wei Wang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Rumen Fluid ◽  
Digestive Enzyme ◽  
Rumen Fermentation ◽  
Dairy Calves ◽  
Node Degree ◽  
Linear Discriminant ◽  
Β Diversity ◽  
Weaning Age ◽  
Fermentation Profile

To better understand the transition of rumen function during the weaning period in dairy calves, sixteen Holstein dairy calves were selected and divided into two groups: pre-weaning (age = 56 ± 7 day, n = 8) and post-weaning (age = 80 ± 6 day, n = 8). The rumen fluid was obtained by an oral gastric tube. The rumen fermentation profile, enzyme activity, bacteria composition, and their inter-relationship were investigated. The results indicated that the post-weaning calves had a higher rumen acetate, propionate, butyrate, and microbial crude protein (MCP) than the pre-weaning calves (p < 0.05). The rumen pH in the post-weaning calves was lower than the pre-weaning calves (p < 0.05). The protease, carboxymethyl cellulase, cellobiohydrolase, and glucosidase in the post-weaning calves had a lower trend than the pre-weaning calves (0.05 < p < 0.1). There was no difference in α and β diversity between the two groups. Linear discriminant analysis showed that the phylum of Fibrobacteres in the post-weaning group was higher than the pre-weaning group. At the genus level, Shuttleworthia, Rikenellaceae, Fibrobacter, and Syntrophococcus could be worked as the unique bacteria in the post-weaning group. The rumen bacteria network node degree in the post-weaning group was higher than the pre-weaning group (16.54 vs. 9.5). The Shuttleworthia genus was highly positively correlated with MCP, propionate, total volatile fatty acid, glucosidase, acetate, and butyrate (r > 0.65, and p < 0.01). Our study provided new information about the rumen enzyme activity and its relationship with bacteria, which help us to better understand the effects of weaning on the rumen function.

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