Inhibition of the lemon brown rot causal agent Phytophthora citrophthora by low‐toxicity compounds

2020 ◽  
Author(s):  
Gabriela M Olmedo ◽  
Carina G Baigorria ◽  
Ana C Ramallo ◽  
Milena Sepulveda ◽  
Jacqueline Ramallo ◽  
...  
Keyword(s):  
Brown Rot ◽  
Causal Agent ◽  
Phytophthora Citrophthora ◽  
Low Toxicity

Effects of Potassium Sorbate on Postharvest Brown Rot of Stone Fruit

2008 ◽  
Vol 71 (8) ◽  
pp. 1626-1631 ◽  
Author(s):  
R. GREGORI ◽  
F. BORSETTI ◽  
F. NERI ◽  
M. MARI ◽  
P. BERTOLINI
Keyword(s):  
Brown Rot ◽  
Quality Parameters ◽  
Potassium Sorbate ◽  
Antifungal Compound ◽  
Kinetic Activity ◽  
Acceptable Quality ◽  
Low Toxicity ◽  
Diffusion Assay ◽  
Sorbate Concentration

The effect of potassium sorbate (K-sorb), a low-toxicity chemical, to control Monilinia spp. was investigated. Preliminary in vitro studies found the MIC of K-sorb for conidial germination and mycelial growth was, respectively, 260 and 1,250 mg/liter. Immersion of naturally infected peach and nectarine fruit in a solution (15 g/liter) of K-sorb for 120 s reduced brown rot by over 80% in four of five trials. Although treated fruits showed a significant reduction in firmness with respect to the control, they did not reach the overripe stage and retained acceptable quality parameters. In an attempt to elucidate the mechanism of action for K-sorb, the inhibition of enzymatic activity by K-sorb was also tested. In a radial diffusion assay, the addition of K-sorb to agarose reduced polygalacturonase (PG) activity across the concentrations considered. The greatest reduction (54.3%, with respect to the control) was obtained at a sorbate concentration of 15 g/liter. PG kinetic activity of Monilinia laxa observed by a spectrophotometric assay peaked after 40 min in all samples tested. PG activity was significantly higher in the control than in the samples with increased K-sorb concentrations. In conclusion, based on these findings, K-sorb can be recommended as a low-toxicity antifungal compound against Monilinia spp. in peaches and nectarines with its mode of action probably depending in part on the inhibition of PG activity in M. laxa.

scholarly journals Molecular detection of Monilinia fructigena as causal agent of brown rot on quince

2012 ◽  
Vol 27 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Jovana Hrustic ◽  
Mila Grahovac ◽  
Milica Mihajlovic ◽  
Goran Delibasic ◽  
Mirko Ivanovic ◽  
...  
Keyword(s):  
Brown Rot ◽  
Causal Agent ◽  
Control Measures ◽  
Fruit Rot ◽  
Specific Primers ◽  
Monilinia Fructigena ◽  
Light Yellow ◽  
Important Host ◽  
Intensive Control ◽  
Apple Fruits

Species of the genus Monilinia are important causal agents of fruit rot on pome and stone fruits in Serbia. The pathogen is very harmful, especially on small properties and cottage plantations where intensive control measures are not applied. Quince is important host for the pathogens of this genus. During spring 2010, intensive occurrence of mummified fruits overwintering on branches of the quince was observed. The pathogen was isolated using standard phytopathological methods. Pathogenicity of eight obtained isolates was tested by artificial inoculation of injured apple fruits. Identification was performed according to pathogenic, morphological and ecological properties, and was confirmed by Multiplex polimeraze chain reaction, PCR. All the isolates studied caused brown rot on inoculated apple fruits. The isolates form light yellow colonies with lobate margins, with single-celled, transparent, elliptical or oval conidia in chains, regardless temperature or light presence. Sclerotia are observed in 14 days old cultures. The highest growth rate of most of the isolates is at 27?C and in dark. Based on studied pathogenic, morphological and ecological characteristics, it was found that the Monilinia fructigena is causal agent of brown rot of quince. Using specific primers (MO368-5, MO368-8R, MO368-10R, Laxa-R2) for detection of Monilinia species in Multiplex PCR reaction, the expected fragment 402 bp in size was amplified, which confirmed that the studied isolates belonged to the species M. fructigena.

STUDY ON BIOCONTROL OF PHYTOPHTHORA CITROPHTHORA, THE CAUSAL AGENT OF PISTACHIO GUMMOSIS

2006 ◽  
pp. 627-630
Author(s):  
R. Saberi-Riseh ◽  
A. Sharifi-Tehrani ◽  
M. Khezri ◽  
M. Ahmadzadeh ◽  
M.J. Nikkhah
Keyword(s):  
Causal Agent ◽  
Phytophthora Citrophthora
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