Using virulence genes hrpB, egl and fliC in differentiation between virulent and avirulent isolates of Ralstonia solanacearum, the causal agent of potato brown rot

2012 ◽  
Vol 6 (2) ◽  
Author(s):  
M. S. Mikhail
Keyword(s):  
Ralstonia Solanacearum ◽  
Virulence Genes ◽  
Brown Rot ◽  
Causal Agent ◽  
Potato Brown Rot

scholarly journals Stenotrophomonas maltophilia: a new potential biocontrol agent of Ralstonia solanacearum, causal agent of potato brown rot

2007 ◽  
Vol 118 (3) ◽  
pp. 211-225 ◽  
Author(s):  
N. A. S. Messiha ◽  
A. D. van Diepeningen ◽  
N. S. Farag ◽  
S. A. Abdallah ◽  
J. D. Janse ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Stenotrophomonas Maltophilia ◽  
Biocontrol Agent ◽  
Brown Rot ◽  
Causal Agent ◽  
Potential Biocontrol Agent ◽  
Potato Brown Rot

Protective Role of Copper Oxide-Streptomycin Nano-drug Against Potato Brown Rot Disease Caused by Ralstonia solanacearum

2021 ◽  
Author(s):  
Mohamed S. Attia ◽  
Naglaa M. Balabel ◽  
Ibtisam M. Ababutain ◽  
Mahmoud S. Osman ◽  
Mohamed M. Nofel ◽  
...  
Keyword(s):  
Copper Oxide ◽  
Ralstonia Solanacearum ◽  
Brown Rot ◽  
Protective Role ◽  
Potato Brown Rot ◽  
Rot Disease

scholarly journals Biological Soil Disinfestation (BSD), a new control method for potato brown rot, caused by Ralstonia solanacearum race 3 biovar 2

2007 ◽  
Vol 117 (4) ◽  
pp. 403-415 ◽  
Author(s):  
Nevein A. S. Messiha ◽  
Anne D. van Diepeningen ◽  
Marcel Wenneker ◽  
Alexander R. van Beuningen ◽  
Jaap D. Janse ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Control Method ◽  
Brown Rot ◽  
Soil Disinfestation ◽  
Biological Soil Disinfestation ◽  
Potato Brown Rot

scholarly journals Genome Resource: Ralstonia solanacearum Phylotype II Sequevar 1 (Race 3 Biovar 2) Strain UW848 From the 2020 U.S. Geranium Introduction

Plant Disease ◽  
2021 ◽  
Vol 105 (1) ◽  
pp. 207-208
Author(s):  
Veronica Roman-Reyna ◽  
Alicia Truchon ◽  
Parul Sharma ◽  
Francesca Peduto Hand ◽  
Reza Mazloom ◽  
...  
Keyword(s):  
United States ◽  
Ralstonia Solanacearum ◽  
Draft Genome ◽  
Brown Rot ◽  
The United States ◽  
Tropical Lowland ◽  
Global Pandemic ◽  
Pandemic Strains ◽  
Potato Brown Rot ◽  
Tomato Bacterial Wilt

Ralstonia solanacearum phylotype II sequevar 1 (RsII-1, formerly race 3 biovar 2) causes tomato bacterial wilt, potato brown rot, and Southern wilt of geranium. Strains in RsII-1 cause wilting in potato and tomato at cooler temperatures than tropical lowland R. solanacearum strains. Although periodically introduced, RsII-1 has not established in the United States. This pathogen is of quarantine concern and listed as a Federal Select Agent. We report a rapidly sequenced (<2 days) draft genome of UW848, a RsII-1 isolate introduced to the United States in geranium cuttings in spring 2020. UW848 belongs to the near-clonal cluster of RsII-1 global pandemic strains.

scholarly journals Ralstonia solanacearum Race 3, Biovar 2 Strains Isolated from Geranium Are Pathogenic on Potato

Plant Disease ◽  
2002 ◽  
Vol 86 (9) ◽  
pp. 987-991 ◽  
Author(s):  
Lynn Williamson ◽  
Kazuhiro Nakaho ◽  
Brian Hudelson ◽  
Caitilyn Allen
Keyword(s):  
United States ◽  
Ralstonia Solanacearum ◽  
Ornamental Plants ◽  
Brown Rot ◽  
The United States ◽  
Enzyme Linked Immunosorbent Assay ◽  
Economic Damage ◽  
Biochemical Tests ◽  
Potato Brown Rot ◽  
Rot Disease

Ralstonia solanacearum race 3, biovar 2 is a soilborne bacterium that causes potato brown rot disease in temperate and subtropical climates. Recent outbreaks of this disease in Europe have caused serious losses, but the pathogen had not been identified in the United States. However, in 1999, strains of R. solanacearum were isolated from wilting geraniums growing in Wisconsin greenhouses. Physiological and biochemical tests of the Wisconsin strains and a similar strain from South Dakota demonstrated that the strains belong to R. solanacearum subgroup biovar 2, which is largely synonymous with the race 3 subgroup, a classification based on host range. These results were confirmed by polymerase chain reaction analyses in which race 3, biovar 2-specific primers amplified a fragment of the expected size. This is the first report of race 3, biovar 2 in the United States, and it is the first known occurrence of race 3, biovar 2 in Wiscon-sin. The geranium strains were highly pathogenic on both geranium and potato. The presence of R. solanacearum race 3, biovar 2 in the United States raises concern that the bacterium could move from ornamental plants into potato fields, where it could cause both direct economic damage and quarantine problems. A commercial indirect enzyme-linked immunosorbent assay for R. solanacearum produced some false negatives for these strains, indicating that current indexing may not be sufficient to identify this destructive pathogen.

scholarly journals Specific Genes from the Potato Brown Rot Strains of Ralstonia solanacearum and Their Potential Use for Strain Detection

2009 ◽  
Vol 99 (9) ◽  
pp. 1105-1112 ◽  
Author(s):  
A. Guidot ◽  
M. Elbaz ◽  
Sébastien Carrère ◽  
M. I. Siri ◽  
M. J. Pianzzola ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Crop Production ◽  
Brown Rot ◽  
Potato Industry ◽  
Genomic Islands ◽  
Detection Methods ◽  
Economic Losses ◽  
Comparative Genomic ◽  
Polymerase Chain ◽  
Potato Brown Rot

Ralstonia solanacearum is the agent of bacterial wilt infecting >200 different plant species covering >50 botanical families. The genus R. solanacearum can be classified into four phylotypes and each phylotype can be further subdivided into sequevars. The potato brown rot strains of R. solanacearum from phylotype IIB, sequevar 1 (IIB1), historically known as race 3, biovar 2 strains, are responsible for important economic losses to the potato industry and threaten ornamental crop production worldwide. Sensitive and specific detection methods are required to control this pathogen. This article provides a list of 70 genes and 15 intergenes specific to the potato brown rot strains of R. solanacearum from phylotype IIB1. This list was identified by comparative genomic hybridization on microarray and subsequent polymerase chain reaction validation with 14 IIB1 strains against 45 non-IIB1 strains that covered the known genetic diversity in R. solanacearum. The microarray used consisted of the previously described microarray representative of the phylotype I strain GMI1000, to which were added 660 70-mer oligonucleotides representative of new genomic islands detected in the phylotype IIB1 strain IPO1609. The brown rot strain-specific genes thus identified were organized in nine clusters covering 2 to 29 genes within the IPO1609 genome and 6 genes isolated along the genome. Of these specific genes, 29 were parts of mobile genetic elements. Considering the known instability of the R. solanacearum genome, we believe that multiple probes are required to consistently detect all IIB1 strains and we recommend the use of probes which are not part of genetic mobile elements.

scholarly journals Genome-Enabled Phylogeographic Investigation of the Quarantine Pathogen Ralstonia solanacearum Race 3 Biovar 2 and Screening for Sources of Resistance Against Its Core Effectors

2015 ◽  
Vol 105 (5) ◽  
pp. 597-607 ◽  
Author(s):  
Christopher R. Clarke ◽  
David J. Studholme ◽  
Byron Hayes ◽  
Brendan Runde ◽  
Alexandra Weisberg ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Ralstonia Solanacearum ◽  
Genetic Resistance ◽  
Brown Rot ◽  
The United States ◽  
South American ◽  
Sources Of Resistance ◽  
Quarantine Pathogen ◽  
Potato Brown Rot ◽  
Core Effectors

Phylogeographic studies inform about routes of pathogen dissemination and are instrumental for improving import/export controls. Genomes of 17 isolates of the bacterial wilt and potato brown rot pathogen Ralstonia solanacearum race 3 biovar 2 (R3bv2), a Select Agent in the United States, were thus analyzed to get insight into the phylogeography of this pathogen. Thirteen of fourteen isolates from Europe, Africa, and Asia were found to belong to a single clonal lineage while isolates from South America were genetically diverse and tended to carry ancestral alleles at the analyzed genomic loci consistent with a South American origin of R3bv2. The R3bv2 isolates share a core repertoire of 31 type III-secreted effector genes representing excellent candidates to be targeted with resistance genes in breeding programs to develop durable disease resistance. Toward this goal, 27 R3bv2 effectors were tested in eggplant, tomato, pepper, tobacco, and lettuce for induction of a hypersensitive-like response indicative of recognition by cognate resistance receptors. Fifteen effectors, eight of them core effectors, triggered a response in one or more plant species. These genotypes may harbor resistance genes that could be identified and mapped, cloned, and expressed in tomato or potato, for which sources of genetic resistance to R3bv2 are extremely limited.

Development of variable number of tandem repeats typing schemes for Ralstonia solanacearum, the agent of bacterial wilt, banana Moko disease and potato brown rot

2013 ◽  
Vol 92 (3) ◽  
pp. 366-374 ◽  
Author(s):  
Carine Aya N'Guessan ◽  
Sylvain Brisse ◽  
Anne-Claire Le Roux-Nio ◽  
Stéphane Poussier ◽  
Daouda Koné ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Tandem Repeats ◽  
Brown Rot ◽  
Variable Number ◽  
Potato Brown Rot
Sign in / Sign up

Export Citation Format

Share Document