scholarly journals Raman spectral signatures of mouse mammary tissue and associated lymph nodes: normal, tumor and mastitis Jagdish S. Thakur, Houbei Dai, Gulay K. Serhatkulu, Ratna Naik, Vaman M. Naik, Alex Cao, Abhilash Pandya, Gregory W. Auner, Rajah Rabah, Michael D. Klein, Carl Freeman.Journal of Raman Spectroscopy, 2007; 38

2007 ◽  
Vol 38 (2) ◽  
pp. i-i
Keyword(s):  
Raman Spectroscopy ◽  
Lymph Nodes ◽  
Mammary Tissue ◽  
Spectral Signatures ◽  
Raman Spectral

scholarly journals Raman spectral signatures of mouse mammary tissue and associated lymph nodes: normal, tumor and mastitis

2007 ◽  
Vol 38 (2) ◽  
pp. 127-134 ◽  
Author(s):  
Jagdish S. Thakur ◽  
Houbei Dai ◽  
Gulay K. Serhatkulu ◽  
Ratna Naik ◽  
Vaman M. Naik ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Mammary Tissue ◽  
Spectral Signatures ◽  
Raman Spectral

Raman Spectral Mapping in the Assessment of Axillary Lymph Nodes in Breast Cancer

2003 ◽  
Vol 2 (4) ◽  
pp. 327-331 ◽  
Author(s):  
J. Smith ◽  
C. Kendall ◽  
A. Sammon ◽  
J. Christie-Brown ◽  
N. Stone
Keyword(s):  
Breast Cancer ◽  
Raman Spectroscopy ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Biological Tissues ◽  
Axillary Lymph Nodes ◽  
Axillary Lymph ◽  
Breast Cancer Patients ◽  
Spectral Mapping ◽  
Raman Spectral

This paper is the first reported description of Raman Spectroscopy in the assessment of axillary lymph nodes in breast cancer patients. Raman Spectroscopy is an inelastic scattering spectroscopic technique appropriate for the assessment of unprocessed complex biological tissues. Spectra represent biochemical signatures of the tissue under scrutiny. The described method of Raman spectral mapping produces false-color spectral images of lymph node sections. These can be compared with standard histopathology slides and white light images of nodal tissue. This method has the potential to allow the detailed biochemical assessment of heterogeneous lymph node features, and to contribute towards the development of an optical diagnostic tool for use in a clinical setting.

Spectroscopic requirements for Raman instrumentation on a planetary lander: potential for the remote detection of biosignatures on Mars

2004 ◽  
Vol 3 (2) ◽  
pp. 165-174 ◽  
Author(s):  
S.E. Jorge Villar ◽  
H.G.M. Edwards
Keyword(s):  
Raman Spectroscopy ◽  
Raman Spectra ◽  
Spectral Resolution ◽  
Detection Sensitivity ◽  
Remote Detection ◽  
Spectral Identification ◽  
Scan Time ◽  
Time Parameters ◽  
Raman Spectral ◽  
Non Destructive

The special characteristics of Raman spectroscopy (relative insensitivity to water, non-destructive detection, sensitivity to bio- and geosignatures, molecular structural composition information, etc.) together with the development of miniaturized Raman spectrometers make the consideration of this technique for future robotic landers on planetary surfaces, particularly Mars, a very interesting option. The development of light and rugged Raman spectrometers limits the possible scope of the instrumentation which has particular importance in the recognition of biomolecular and mineral signatures. In this work, we evaluate the spectral resolution and scan time parameters and the effect that they have on the Raman spectra of extremophilic biomolecules, together with the wavenumber ranges which are critical for the detection of life signals. This is of vital relevance for the design of miniaturized Raman spectrometer systems. From our results, we conclude that for extraterrestrial biological signatures unambiguous Raman spectral identification provided with a minimum of 16 cm−1 spectral resolution is required for the most significant biosignature wavenumber range in the 1700–700 cm−1 region.

Raman spectral analysis of nasopharyngeal carcinoma cell line CNE2 after microwave radiation

2013 ◽  
Vol 91 (2) ◽  
pp. 67-71 ◽  
Author(s):  
Yuhuang Ye ◽  
Yang Chen ◽  
Ying Su ◽  
Changyan Zou ◽  
Yangwen Huang ◽  
...  
Keyword(s):  
Support Vector Machine ◽  
Raman Spectroscopy ◽  
Nasopharyngeal Carcinoma ◽  
Microwave Radiation ◽  
Principal Components ◽  
Carcinoma Cell ◽  
Support Vector ◽  
Components Analysis ◽  
Raman Spectral ◽  
Radiation Time

This study aimed to study the effects of microwave radiation on the nasopharyngeal carcinoma cell line CNE2 by Raman spectroscopy. The cells were separated into a control group and radiated groups with radiation times of 2, 5, 10, and 25 min, respectively. Both principal components analysis and support vector machine were employed for statistical analysis of Raman spectra. The results show that the relative content of C-H deformation and amide I begin to change when the radiation time is over 10 min, and principal components analysis further confirms there are significant differences after 10 min of radiation. Moreover, support vector machine is simultaneously used to classify radiated samples from control samples. The classification accuracy is low until the radiation time reaches over 10 min. In conclusion, this study reveals the Raman spectral characteristics of CNE2 under different microwave radiation exposure timesand demonstrates Raman spectroscopy can be a potential method to explore cellular characterization after radiation. The final results may help in elucidating the mechanism by which microwave radiation interacts with tumor cells.

scholarly journals Investigation on the Cancer Invasion and Metastasis of Skin Squamous Cell Carcinoma by Raman Spectroscopy

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2059 ◽  
Author(s):  
Xu Zhang ◽  
Fan Yu ◽  
Jie Li ◽  
Dongliang Song ◽  
Heping Li ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Biological Information ◽  
Skin Squamous Cell Carcinoma ◽  
Cancer Field ◽  
Hematoxylin And Eosin ◽  
Raman Spectral

Raman spectroscopy facilitates accurate and minimally invasive investigation on biomedical samples to reveal their molecular-level biological information. In this work, the cancer field effects of squamous cell carcinoma (SCC) tissues were illustrated by Raman microspectroscopy. Referenced with hematoxylin and eosin (H&E) stained microscopic images, the biochemical variations during SCC progress were meticulously described by the Raman spectral features in different pathological areas of two lesion types, including the biochemical changes in collagen, lipids, DNA, and other components of SCC diffusion and metastasis. The experimental results demonstrated that the intensities of the Raman peaks representing collagen (853, 936, and 1248 cm−1) were decreased, whereas the intensities of peaks corresponding to DNA (720, 1327 cm−1) and lipids (1305 cm−1) were increased significantly in cancerous lesions, which testified that SCC originates from the epidermis and invades the dermis gradually. The achieved results not only described the molecular mechanism of skin carcinogenesis, but also provided vital reference data for in vivo skin cancer diagnosis using Raman spectroscopy.

Raman Spectroscopy: A Promising Method of Assessing Axillary Lymph Nodes Even in the Presence of Blue Dye

2010 ◽  
Vol 36 (11) ◽  
pp. 1111
Author(s):  
Jonathan Horsnell ◽  
P. Stonelake ◽  
G. Shetty ◽  
J. Christie-Brown ◽  
C. Kendall ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Lymph Nodes ◽  
Promising Method ◽  
Axillary Lymph Nodes ◽  
Blue Dye ◽  
Axillary Lymph

Raman Spectral Characterization of Pure and Doped Fused Silica Optical Fibers

1975 ◽  
Vol 29 (4) ◽  
pp. 337-344 ◽  
Author(s):  
G. E. Walrafen ◽  
J. Stone
Keyword(s):  
Raman Spectroscopy ◽  
Raman Spectra ◽  
Optical Fibers ◽  
Fused Silica ◽  
Laser Raman ◽  
Laser Raman Spectra ◽  
Raman Spectral ◽  
Oh Content ◽  
Absorption Measurements

The utility of Raman spectroscopy as a means of characterizing the properties of pure and doped fused silica has been investigated. Laser-Raman spectra were obtained by forward scattering from solid optical fibers ∼35 to 85 m in length using 514.5 nm excitation with an “image slicer” and a Cary model 81 instrument. Clad and unclad fibers of fused silica and doped fibers having SiO2-GeO2 and SiO2-GeO2-B2O3 cores were examined. Raman spectra were also obtained from bulk samples of glasses, including pure GeO2, pure B2O3, and various compositions of SiO2-GeO2, SiO2-B2O3, and SiO2-GeO2-B2O3. The addition of dopants to fused silica was found to alter the Raman spectrum both by the appearance of new bands, roughly proportional to dopant concentration and not common either to the fused silica or to the dopant alone, and by the marked alteration of other Raman bands, which is indicative of changes in the local intermolecular order. Thus, addition of GeO2 produces new Raman bands at ∼675 and ∼1000 cm−1; and of B2O3, new bands at ∼940 and ∼1350 cm−1. Addition of GeO2 and/or B2O3 weakens the relatively sharp Raman lines near 485 and 600 cm−1 (and a similar but small effect was also noted with increasing OH content). GeO2 and B2O3 together also produce observable narrowing of the broad intense 440 cm−1 Raman contour. These spectral effects are interpreted, respectively, in terms of a decrease in the concentrations of [Formula: see text] and [Formula: see text] defects produced by dopant addition and of a concomitant reordering of the silica structure. Raman spectroscopy thus appears to be a useful optical technique for elucidating the properties of dopants that have been especially chosen for good optical transmission and hence are not easily detectable by absorption measurements.

Raman Spectroscopy Combined with Principal Component Analysis for Screening Nasopharyngeal Cancer in Human Blood Sera

2017 ◽  
Vol 71 (11) ◽  
pp. 2497-2503 ◽  
Author(s):  
Saranjam Khan ◽  
Rahat Ullah ◽  
Samina Javaid ◽  
Shaheen Shahzad ◽  
Hina Ali ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Raman Spectroscopy ◽  
Human Blood ◽  
Nasopharyngeal Cancer ◽  
Principal Component ◽  
Component Analysis ◽  
Data Sets ◽  
Analysis Technique ◽  
Raman Spectral Data ◽  
Raman Spectral

This study demonstrates the analysis of nasopharyngeal cancer (NPC) in human blood sera using Raman spectroscopy combined with the multivariate analysis technique. Blood samples of confirmed NPC patients and healthy individuals have been used in this study. The Raman spectra from all these samples were recorded using 785 nm laser for excitation. Important Raman bands at 760, 800, 815, 834, 855, 1003, 1220–1275, and 1524 cm−1, have been observed in both normal and NPC samples. A decrease in the lipids content, phenylalanine, and β-carotene, whereas increases in amide III, tyrosine, and tryptophan have been observed in the NPC samples. The two data sets were well separated using principal component analysis (PCA) based on Raman spectral data. The spectral variations between the healthy and cancerous samples have been further highlighted by plotting loading vectors PC1 and PC2, which shows only those spectral regions where the differences are obvious.

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