Efficient and High-Speed Transduction of an Antibody into Living Cells Using a Multifunctional Nanocarrier System to Control Intracellular Trafficking

2015 ◽  
Vol 104 (9) ◽  
pp. 2845-2854 ◽  
Author(s):  
Yuma Yamada ◽  
Sandra Milena Vergara Perez ◽  
Mai Tabata ◽  
Jiro Abe ◽  
Yukari Yasuzaki ◽  
...  
Keyword(s):  
Intracellular Trafficking ◽  
High Speed ◽  
Living Cells

Intracellular trafficking and cytoplasmic streaming under abiotic stress conditions

2019 ◽  
Vol 6 (04) ◽  
Author(s):  
JESHIMA KHAN YASIN ◽  
ANIL KUMAR SINGH
Keyword(s):  
Plasma Membrane ◽  
Abiotic Stress ◽  
Confocal Microscopy ◽  
Fusion Protein ◽  
Intracellular Trafficking ◽  
Cytoplasmic Streaming ◽  
Living Cells ◽  
Stress Conditions ◽  
Vesicle Formation ◽  
External Stimuli

Cytoplasmic streaming is one among the vital activities of the living cells. In plants cytolplasmic streaming could clearly be seen in hypocotyls of growing seedlings. To observe cytoplsmic streaming and its correlated intracellular trafficking an investigation was conducted in legumes in comparison with GFP-AtRab75 and 35S::GFP:δTIP tonoplast fusion protein expressing arabidopsis lines. These seedlings were observed under confocal microscopy with different buffer incubation treatments and under different stress conditions. GFP expressing 35S::GFP:δTIP tonoplast lines were looking similar to the control lines and differ under stress conditions. Movement of cytoplasmic invaginations within the tonoplast and cytoplasmic sub vesicle or bulb budding during cytoplasmic streaming was observed in hypocotyls of At-GFP tonoplast plants. We found the cytoplasmic bulbs/ vesicles or sub vesicle formation from the plasma membrane. The streaming speed also depends on the incubation medium in which the specimen was incubated, indicating that the external stimuli as well as internal stimuli can alter the speed of streaming

14-3-3 Facilitates Insulin-Stimulated Intracellular Trafficking of Insulin Receptor Substrate 1

2002 ◽  
Vol 16 (3) ◽  
pp. 552-562 ◽  
Author(s):  
Xiaoqin Xiang ◽  
Mingsheng Yuan ◽  
Ying Song ◽  
Neil Ruderman ◽  
Rong Wen ◽  
...  
Keyword(s):  
Insulin Receptor ◽  
Intracellular Trafficking ◽  
High Speed ◽  
Insulin Treatment ◽  
Insulin Receptor Substrate ◽  
Insulin Receptor Substrate 1 ◽  
Integral Role ◽  
Endogenous Proteins

Abstract The appearance of a complex between tyrosine-phosphorylated insulin receptor substrate 1 (IRS-1) and PI3K in a high-speed pellet fraction (HSP) is thought to be a key event in insulin action. Conversely, the disappearance of the IRS-1/PI3K complex from this fraction has been linked to insulin desensitization. The present study examines the role of 14-3-3, a specific phospho-serine binding protein, in mediating the disappearance of IRS-1 from the HSP after insulin treatment. An in vitro pull-down assay using recombinant 14-3-3 revealed that insulin enhances the association of 14-3-3 with IRS-1 in cultured adipocytes and that this is completely inhibited by wortmannin. An association of IRS-1 and 14-3-3 was also observed and was maximal after stimulation by insulin, when endogenous proteins were immunoprecipitated. Epidermal growth factor (EGF), 12-O-tetradecanoylphorbol-13-acetate, and okadaic acid, other agents that cause serine/threonine phosphorylation of IRS-1, also stimulated IRS binding to 14-3-3. The enhancement of IRS-1 binding to 14-3-3 by insulin was accompanied by movement of IRS-1 and the p85 subunit of PI3K from the HSP to the cytosol. In keeping with a key role of 14-3-3 in mediating this redistribution of IRS-1, the complexes of IRS-1 and 14-3-3 were found in the cytosol but not in the HSP of insulin-treated cells. In addition, colocalization of IRS-1 and 14-3-3 was observed in the cytoplasm after insulin treatment by confocal microscopy. Finally, the addition of a phosphorylated 14-3-3 binding peptide to an adipocyte homogenate (to remove 14-3-3 from IRS-1) increased the abundance of IRS-1/PI3K complexes in the HSP and decreased their abundance in the cytosol. These findings strongly suggest that 14-3-3 participates in the intracellular trafficking of IRS-1 by promoting the displacement of serine-phosphorylated IRS-1 from particular structures. They also suggest that 14-3-3 proteins could play an integral role in the process of insulin desensitization.

High-speed AFM for scanning the architecture of living cells

Nanoscale ◽  
2013 ◽  
Vol 5 (18) ◽  
pp. 8355 ◽  
Author(s):  
Jing Li ◽  
Zhifeng Deng ◽  
Daixie Chen ◽  
Zhuo Ao ◽  
Quanmei Sun ◽  
...  
Keyword(s):  
High Speed ◽  
Living Cells

scholarly journals HIGH SPEED PHOTOMICROGRAPHY OF LIVING CELLS SUBJECTED TO SUPERSONIC VIBRATIONS

1931 ◽  
Vol 15 (2) ◽  
pp. 147-153 ◽  
Author(s):  
E. Newton Harvey ◽  
Alfred L. Loomis
Keyword(s):  
High Frequency ◽  
High Speed ◽  
Living Cells ◽  
Air Bubbles ◽  
Sound Waves ◽  
Camera System ◽  
Fluid Movement ◽  
High Frequency Sound ◽  
Frequency Sound ◽  
New Type

A new type of camera system is described capable of taking 1200 pictures a second through a microscope objective. Photographs showing the destruction of Arbacia eggs by high frequency sound waves indicate that the disintegration occurs in less than 1/1200 second. Eggs drawn out into spindle or tadpole shapes suggest that rapid movements of the fluid tearing the eggs may be responsible for the disintegration. Although no cavitated air bubbles show in the photographs, other experiments make it likely that the rapid fluid movement is the result of submicroscopic cavitation.

A high-speed vertical optical trap for the mechanical testing of living cells at piconewton forces

2013 ◽  
Vol 84 (11) ◽  
pp. 113707 ◽  
Author(s):  
Kai Bodensiek ◽  
Weixing Li ◽  
Paula Sánchez ◽  
Schanila Nawaz ◽  
Iwan A. T. Schaap
Keyword(s):  
Mechanical Testing ◽  
High Speed ◽  
Optical Trap ◽  
Living Cells

Simultaneous vibration and high-speed microscopy to study mechanotransduction in living cells

2012 ◽  
Author(s):  
David W. Holdsworth ◽  
Hristo N. Nikolov ◽  
Jen Au ◽  
Kim Beaucage ◽  
Jessica Kishimoto ◽  
...  
Keyword(s):  
High Speed ◽  
Living Cells

Dendrimer Internalization and Intracellular Trafficking in Living Cells

2010 ◽  
Vol 7 (3) ◽  
pp. 680-688 ◽  
Author(s):  
Lorenzo Albertazzi ◽  
Michela Serresi ◽  
Alberto Albanese ◽  
Fabio Beltram
Keyword(s):  
Intracellular Trafficking ◽  
Living Cells

scholarly journals Intracellular trafficking of cationic liposome–DNA complexes in living cells

Soft Matter ◽  
2012 ◽  
Vol 8 (30) ◽  
pp. 7919 ◽  
Author(s):  
Stefano Coppola ◽  
Laura C. Estrada ◽  
Michelle A. Digman ◽  
Daniela Pozzi ◽  
Francesco Cardarelli ◽  
...  
Keyword(s):  
Intracellular Trafficking ◽  
Cationic Liposome ◽  
Living Cells ◽  
Dna Complexes
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