scholarly journals Osteochondral repair using an acellular dermal matrix-pilot in vivo study in a rabbit osteochondral defect model

2018 ◽  
Vol 36 (7) ◽  
pp. 1919-1928 ◽  
Author(s):  
Ken Ye ◽  
Kathy Traianedes ◽  
Shalley A. Robins ◽  
Peter F. M. Choong ◽  
Damian E. Myers
Keyword(s):  
Osteochondral Defect ◽  
Acellular Dermal Matrix ◽  
In Vivo Study ◽  
Defect Model ◽  
Dermal Matrix ◽  
Osteochondral Repair ◽  
Acellular Dermal

scholarly journals Multiphasic construct studied in an ectopic osteochondral defect model

2014 ◽  
Vol 11 (95) ◽  
pp. 20140184 ◽  
Author(s):  
June E. Jeon ◽  
Cédryck Vaquette ◽  
Christina Theodoropoulos ◽  
Travis J. Klein ◽  
Dietmar W. Hutmacher
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Osteochondral Defect ◽  
Low Cost ◽  
Current Model ◽  
Large Animal ◽  
Micro Computed Tomography ◽  
Defect Model ◽  
Osteochondral Repair

In vivo osteochondral defect models predominantly consist of small animals, such as rabbits. Although they have an advantage of low cost and manageability, their joints are smaller and more easily healed compared with larger animals or humans. We hypothesized that osteochondral cores from large animals can be implanted subcutaneously in rats to create an ectopic osteochondral defect model for routine and high-throughput screening of multiphasic scaffold designs and/or tissue-engineered constructs (TECs). Bovine osteochondral plugs with 4 mm diameter osteochondral defect were fitted with novel multiphasic osteochondral grafts composed of chondrocyte-seeded alginate gels and osteoblast-seeded polycaprolactone scaffolds, prior to being implanted in rats subcutaneously with bone morphogenic protein-7. After 12 weeks of in vivo implantation, histological and micro-computed tomography analyses demonstrated that TECs are susceptible to mineralization. Additionally, there was limited bone formation in the scaffold. These results suggest that the current model requires optimization to facilitate robust bone regeneration and vascular infiltration into the defect site. Taken together, this study provides a proof-of-concept for a high-throughput osteochondral defect model. With further optimization, the presented hybrid in vivo model may address the growing need for a cost-effective way to screen osteochondral repair strategies before moving to large animal preclinical trials.

scholarly journals Non-Cross-Linked Collagen Mesh Performs Best in a Physiologic, Noncontaminated Rat Model

2019 ◽  
Vol 26 (3) ◽  
pp. 302-311 ◽  
Author(s):  
Ruth Kaufmann ◽  
An P. Jairam ◽  
Irene M. Mulder ◽  
Zhouqiao Wu ◽  
Joost Verhelst ◽  
...  
Keyword(s):  
Rat Model ◽  
Incisional Hernia Repair ◽  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Laparoscopic Incisional Hernia Repair ◽  
Mesh Shrinkage ◽  
Male Wistar Rats ◽  
Porcine Acellular Dermal Matrix ◽  
Acellular Dermal

Background. In laparoscopic incisional hernia repair, direct contact between the prosthesis and abdominal viscera is inevitable and may lead to adhesions. Despite the large variety of mesh prosthesis, little is known about their in vivo behavior. Biological meshes are considered to have many advantages, but due to their price they are rarely used. A rat model was used to assess biological and conventional synthetic meshes on their in vivo characteristics. Design. One-hundred twenty male Wistar rats were randomized into five groups of 24 rats. A mesh was implanted intraperitoneally and fixated with nonresorbable sutures. The following five meshes were implanted: Parietene (polypropylene), Permacol (cross-linked porcine acellular dermal matrix), Strattice (non-cross-linked porcine acellular dermal matrix), XCM Biologic (non-cross-linked porcine acellular dermal matrix), and Omyra Mesh (condensed polytetrafluoroethylene). The rats were sacrificed after 30, 90, or 180 days. Incorporation, shrinkage, adhesions, abscess formation, and histology were assessed for all meshes. Results. All animals thrived postoperatively. After 180 days, Permacol, Parietene, and Omyra Mesh had a significantly better incorporation than Strattice ( P = .001, P = .019, and P = .037 respectively). After 180 days, Strattice had significantly fewer adhesions on the surface of the mesh than Parietene ( P < .001), Omyra Mesh ( P = .011), and Permacol ( P = .027). After 30 days, Permacol had significantly stronger adhesions than Strattice ( P = .030). However, this difference was not significant anymore after 180 days. After 180 days, there was significantly less shrinkage in Permacol than in Strattice ( P = .001) and Omyra Mesh ( P = .050). Conclusion. Based on incorporation, adhesions, mesh shrinkage, and histologic parameters, Strattice performed best in this experimental rat model.

Construction of a dermis–fat composite in vivo: Optimizing heterogeneous acellular dermal matrix with in vitro pretreatment

2019 ◽  
Vol 14 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Zhu Zhu ◽  
Zhao‐Qi Yuan ◽  
Cheng Huang ◽  
Rui Jin ◽  
Di Sun ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Acellular Dermal
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