Low-level laser irradiation promotes cell proliferation and mRNA expression of type I collagen and decorin in porcine achilles tendon fibroblastsIn Vitro

2009 ◽  
Vol 27 (5) ◽  
pp. 646-650 ◽  
Author(s):  
Chia-Hsin Chen ◽  
Jin-Lian Tsai ◽  
Yan-Hsiung Wang ◽  
Chia-Ling Lee ◽  
June-Kai Chen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Achilles Tendon ◽  
Mrna Expression ◽  
Laser Irradiation ◽  
Type I Collagen ◽  
Type I ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation

scholarly journals Low-level laser irradiation promotes proliferation of cryopreserved adipose-derived stem cells

2017 ◽  
Vol 15 (3) ◽  
pp. 334-338 ◽  
Author(s):  
Fernanda Ginani ◽  
Diego Moura Soares ◽  
Hugo Alexandre de Oliveira Rocha ◽  
Carlos Augusto Galvão Barboza
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Cell Proliferation ◽  
Laser Irradiation ◽  
Adipose Derived Stem Cells ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Significant Difference ◽  
Low Level Laser Irradiation

ABSTRACT Objective To evaluate the effect of low-level laser irradiation on proliferation and viability of murine adipose-derived stem cells previously submitted to cryopreservation. Methods Adipose-derived stem cells were isolated from inguinal fat pads of three mice, submitted to cryopreservation in fetal bovine serum with 10% dimethylsulfoxide for 30 days and then thawed and maintained in normal culture conditions. Culture cells were either irradiated or not (control) with an InGaAIP diode laser at zero and 48 hours, using two different energy densities (0.5 and 1.0J/cm2). Cell proliferation was evaluated by trypan blue exclusion method and MTT assay at intervals of zero, 24, 48, and 72 hours after the first laser application. Cell viability and apoptosis of previously cryopreserved cells submitted to laser therapy were evaluated by flow cytometry. Results The Irradiated Groups (0.5 and 1.0J/cm2) showed an increased cell proliferation (p<0.05) when compared to the Control Group, however no significant difference between the two energy densities was observed. Flow cytometry revealed a percentage of viable cells higher than 99% in all groups. Conclusion Low-level laser irradiation has stimulatory effects on the proliferation of adipose-derived stem cells previously submitted to cryopreservation.

Low-level laser irradiation facilitates fibronectin and collagen type I turnover during tooth movement in rats

2008 ◽  
Vol 25 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Yong-Deok Kim ◽  
Seong-Sik Kim ◽  
Seok-Jun Kim ◽  
Dae-Woo Kwon ◽  
Eun-Suk Jeon ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Collagen Type ◽  
Tooth Movement ◽  
Collagen Type I ◽  
Type I ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation

Low-level laser irradiation alters mRNA expression from genes involved in DNA repair and genomic stabilization in myoblasts

2016 ◽  
Vol 13 (7) ◽  
pp. 075601 ◽  
Author(s):  
L A S N Trajano ◽  
L P S Sergio ◽  
C L Silva ◽  
L Carvalho ◽  
A L Mencalha ◽  
...  
Keyword(s):  
Dna Repair ◽  
Mrna Expression ◽  
Laser Irradiation ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation

Low-Level Laser Irradiation (InGaAlP-660 nm) Increases Fibroblast Cell Proliferation and Reduces Cell Death in a Dose-Dependent Manner

2010 ◽  
Vol 28 (S1) ◽  
pp. S-151-S-156 ◽  
Author(s):  
Lucio Frigo ◽  
Giovani M. Fávero ◽  
Haroldo J. Campos Lima ◽  
Durvanei Augusto Maria ◽  
Jan M. Bjordal ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Laser Irradiation ◽  
Fibroblast Cell ◽  
Dependent Manner ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation ◽  
Dose Dependent
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