scholarly journals In vivo bioluminescence imaging study to monitor ectopic bone formation by luciferase gene marked mesenchymal stem cells

2008 ◽  
Vol 26 (7) ◽  
pp. 901-909 ◽  
Author(s):  
Cristina Olivo ◽  
Jacqueline Alblas ◽  
Vivienne Verweij ◽  
Anton-Jan Van Zonneveld ◽  
Wouter J. A. Dhert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bioluminescence Imaging ◽  
Imaging Study ◽  
Ectopic Bone Formation ◽  
Luciferase Gene ◽  
Ectopic Bone ◽  
In Vivo Bioluminescence Imaging

Alginate Bead-Encapsulated PEDF Induces Ectopic Bone Formation In Vivo in the Absence of Co-Administered Mesenchymal Stem Cells

2018 ◽  
Vol 19 (5) ◽  
pp. 467-478 ◽  
Author(s):  
Mina Elahy ◽  
Michael R. Doschak ◽  
Jeffery D. Hughes ◽  
Swati Baindur-Hudson ◽  
Crispin R. Dass
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Alginate Bead ◽  
Ectopic Bone Formation ◽  
Ectopic Bone

scholarly journals NEL-Like Molecule-1 (Nell1) Is Regulated by Bone Morphogenetic Protein 9 (BMP9) and Potentiates BMP9-Induced Osteogenic Differentiation at the Expense of Adipogenesis in Mesenchymal Stem Cells

2017 ◽  
Vol 41 (2) ◽  
pp. 484-500 ◽  
Author(s):  
Jing Wang ◽  
Junyi Liao ◽  
Fugui Zhang ◽  
Dongzhe Song ◽  
Minpeng Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Adipogenic Differentiation ◽  
Ectopic Bone Formation ◽  
Morphogenetic Protein ◽  
Ectopic Bone ◽  
Bone Morphogenetic Protein 9

Background: BMP9 induces both osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs). Nell1 is a secretory glycoprotein with osteoinductive and anti-adipogenic activities. We investigated the role of Nell1 in BMP9-induced osteogenesis and adipogenesis in MSCs. Methods: Previously characterized MSCs iMEFs were used. Overexpression of BMP9 and NELL1 or silencing of mouse Nell1 was mediated by adenoviral vectors. Early and late osteogenic and adipogenic markers were assessed by staining techniques and qPCR analysis. In vivo activity was assessed in an ectopic bone formation model of athymic mice. Results: We demonstrate that Nell1 expression was up-regulated by BMP9. Exogenous Nell1 potentiated BMP9-induced late stage osteogenic differentiation while inhibiting the early osteogenic marker. Forced Nell1 expression enhanced BMP9-induced osteogenic regulators/markers and inhibited BMP9-upregulated expression of adipogenic regulators/markers in MSCs. In vivo ectopic bone formation assay showed that exogenous Nell1 expression enhanced mineralization and maturity of BMP9-induced bone formation, while inhibiting BMP9-induced adipogenesis. Conversely, silencing Nell1 expression in BMP9-stimulated MSCs led to forming immature chondroid-like matrix. Conclusion: Our findings indicate that Nell1 can be up-regulated by BMP9, which in turn accelerates and augments BMP9-induced osteogenesis. Exogenous Nell1 may be exploited to enhance BMP9-induced bone formation while overcoming BMP9-induced adipogenesis in regenerative medicine.

scholarly journals The Use of Injectable Chitosan/Nanohydroxyapatite/Collagen Composites with Bone Marrow Mesenchymal Stem Cells to Promote Ectopic Bone Formation In Vivo

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Bo Yu ◽  
Yichen Zhang ◽  
Xiaoming Li ◽  
Qiongren Wang ◽  
Yi Ouyang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Structure ◽  
Ectopic Bone Formation ◽  
Ct Reconstruction ◽  
Bone Mesenchymal Stem Cells ◽  
Inflammatory Reactions ◽  
Ectopic Bone

The aim of this study was to evaluate ectopic in vivo bone formation with or without rat bone mesenchymal stem cells (rBMSCs) of an injectable Chitosan/Nanohydroxyapatite/Collagen (CS/nHAC) composite. The CS/nHAC composites were injected subcutaneously into the backs of Wistar rats with freshly loaded rBMSCs at a density of10×106 cells/mL, and the CS/nHAC composites without cells were used as negative controls. New bone formation, degradation of composites, and degree of calcification were evaluated by Computed Tomography (CT) and three-dimensional (3D) CT reconstruction. Histological evaluations were performed to further assess bone structure and extracellular matrix by HE and Masson staining. The inflammatory reactions related to osteogenesis were also investigated in the present study. In comparison with the CS/nHAC composites, this study revealed that CS/nHAC/rBMSCs composites showed relatively higher percentage of calcification, better establishment of ECM, and less degradation rate. Meanwhile, different extents of inflammatory reactions were also observed in the CS/nHAC and CS/nHAC/rBMSCs explants at 2 and 4 weeks after implantation. Altogether, CS/nHAC/rBMSCs composites are superior to CS/nHAC composites in ectopic bone formation. In conclusion, the rBMSCs-seeded CS/nHAC composites may be beneficial to enhancing ectopic bone formation in vivo.

scholarly journals In Vivo Bioluminescence Imaging of Transplanted Mesenchymal Stem Cells as a Potential Source for Pancreatic Regeneration

2014 ◽  
Vol 13 (8) ◽  
pp. 7290.2014.00023 ◽  
Author(s):  
Song Lee ◽  
Hyewon Youn ◽  
Taemoon Chung ◽  
Do Won Hwang ◽  
So Won Oh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bioluminescence Imaging ◽  
Pancreatic Regeneration ◽  
Potential Source ◽  
In Vivo Bioluminescence Imaging

scholarly journals Hybrid Biomaterial with Conjugated Growth Factors and Mesenchymal Stem Cells for Ectopic Bone Formation

2016 ◽  
Vol 22 (13-14) ◽  
pp. 928-939 ◽  
Author(s):  
Xue Yuan ◽  
Randall J. Smith ◽  
Huiyan Guan ◽  
Ciprian N. Ionita ◽  
Parag Khobragade ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Bone Formation ◽  
Ectopic Bone Formation ◽  
Ectopic Bone
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