Osteogenic Differentiation and Ectopic Bone Formation of Canine Bone Marrow-Derived Mesenchymal Stem Cells in Injectable Thermo-Responsive Polymer Hydrogel

2011 ◽  
Vol 17 (11) ◽  
pp. 1139-1149 ◽  
Author(s):  
Han-Tsung Liao ◽  
Chien-Tzung Chen ◽  
Jyh-Ping Chen
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Ectopic Bone Formation ◽  
Polymer Hydrogel ◽  
Ectopic Bone ◽  
Responsive Polymer

scholarly journals BMP9 exhibits dual and coupled roles in inducing osteogenic and angiogenic differentiation of mesenchymal stem cells

2020 ◽  
Vol 40 (6) ◽  
Author(s):  
Haozhuo Xiao ◽  
Xiaoyu Wang ◽  
Claire Wang ◽  
Guangming Dai ◽  
Zhenglin Zhu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Blood Vessel ◽  
Osteogenic Differentiation ◽  
Ectopic Bone Formation ◽  
Blood Vessel Formation ◽  
Vessel Formation ◽  
Protein Levels ◽  
Ectopic Bone

Abstract Bone morphogenetic protein (BMP) 9 (BMP9) is one of most potent BMPs in inducing osteogenic differentiation of mesenchymal stem cells (MSCs). Recently, evidence has shown that osteogenesis and angiogenesis are coupled, however, it is unclear whether BMP9 induces MSC differentiation into endothelial-like cells and further promotes blood vessel formation. In the present study, we explored the potential of BMP9-induced angiogenic differentiation of MSCs, and the relationship between BMP9-induced osteogenic and angiogenic differentiation of MSCs. Osteogenic activities and angiogenic differentiation markers were analyzed at mRNA and protein levels. In vivo osteogenic and angiogenic differentiation of MSCs were tested by the ectopic bone formation model. We identified that adenoviral vectors effectively transduced in immortalized mouse embryonic fibroblasts (iMEFs) and expressed BMP9 with high efficiency. We found that BMP9 induces early and late osteogenic differentiation, and it up-regulated osteogenic marker expression in MSCs. Meanwhile, BMP9 induces angiogenic differentiation of MSCs via the expression of vascular endothelial growth factor a (VEGFa) and CD31 at both mRNA and protein levels. CD31-positive cells were also increased with the stimulation of BMP9. The ectopic bone formation tests found that BMP9-induced trabecular bone formation was coupled with the expression of blood vessel formation markers and sinusoid capillary formation. These findings suggest that BMP9 exhibits dual and coupled roles in inducing osteogenic and angiogenic differentiation of MSCs.

scholarly journals NEL-Like Molecule-1 (Nell1) Is Regulated by Bone Morphogenetic Protein 9 (BMP9) and Potentiates BMP9-Induced Osteogenic Differentiation at the Expense of Adipogenesis in Mesenchymal Stem Cells

2017 ◽  
Vol 41 (2) ◽  
pp. 484-500 ◽  
Author(s):  
Jing Wang ◽  
Junyi Liao ◽  
Fugui Zhang ◽  
Dongzhe Song ◽  
Minpeng Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Adipogenic Differentiation ◽  
Ectopic Bone Formation ◽  
Morphogenetic Protein ◽  
Ectopic Bone ◽  
Bone Morphogenetic Protein 9

Background: BMP9 induces both osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs). Nell1 is a secretory glycoprotein with osteoinductive and anti-adipogenic activities. We investigated the role of Nell1 in BMP9-induced osteogenesis and adipogenesis in MSCs. Methods: Previously characterized MSCs iMEFs were used. Overexpression of BMP9 and NELL1 or silencing of mouse Nell1 was mediated by adenoviral vectors. Early and late osteogenic and adipogenic markers were assessed by staining techniques and qPCR analysis. In vivo activity was assessed in an ectopic bone formation model of athymic mice. Results: We demonstrate that Nell1 expression was up-regulated by BMP9. Exogenous Nell1 potentiated BMP9-induced late stage osteogenic differentiation while inhibiting the early osteogenic marker. Forced Nell1 expression enhanced BMP9-induced osteogenic regulators/markers and inhibited BMP9-upregulated expression of adipogenic regulators/markers in MSCs. In vivo ectopic bone formation assay showed that exogenous Nell1 expression enhanced mineralization and maturity of BMP9-induced bone formation, while inhibiting BMP9-induced adipogenesis. Conversely, silencing Nell1 expression in BMP9-stimulated MSCs led to forming immature chondroid-like matrix. Conclusion: Our findings indicate that Nell1 can be up-regulated by BMP9, which in turn accelerates and augments BMP9-induced osteogenesis. Exogenous Nell1 may be exploited to enhance BMP9-induced bone formation while overcoming BMP9-induced adipogenesis in regenerative medicine.

scholarly journals Ectopic bone formation by aggregated mesenchymal stem cells from bone marrow and adipose tissue: A comparative study

2017 ◽  
Vol 12 (1) ◽  
pp. e150-e158 ◽  
Author(s):  
Eelco M. Fennema ◽  
Laurent A.H. Tchang ◽  
Huipin Yuan ◽  
Clemens A. van Blitterswijk ◽  
Ivan Martin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Bone Formation ◽  
Comparative Study ◽  
Ectopic Bone Formation ◽  
Ectopic Bone

scholarly journals In vivo bioluminescence imaging study to monitor ectopic bone formation by luciferase gene marked mesenchymal stem cells

2008 ◽  
Vol 26 (7) ◽  
pp. 901-909 ◽  
Author(s):  
Cristina Olivo ◽  
Jacqueline Alblas ◽  
Vivienne Verweij ◽  
Anton-Jan Van Zonneveld ◽  
Wouter J. A. Dhert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bioluminescence Imaging ◽  
Imaging Study ◽  
Ectopic Bone Formation ◽  
Luciferase Gene ◽  
Ectopic Bone ◽  
In Vivo Bioluminescence Imaging

Alginate Bead-Encapsulated PEDF Induces Ectopic Bone Formation In Vivo in the Absence of Co-Administered Mesenchymal Stem Cells

2018 ◽  
Vol 19 (5) ◽  
pp. 467-478 ◽  
Author(s):  
Mina Elahy ◽  
Michael R. Doschak ◽  
Jeffery D. Hughes ◽  
Swati Baindur-Hudson ◽  
Crispin R. Dass
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Alginate Bead ◽  
Ectopic Bone Formation ◽  
Ectopic Bone
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