scholarly journals In vivo bioluminescence imaging of magnetically targeted bone marrow-derived mesenchymal stem cells in skeletal muscle injury model

2012 ◽  
Vol 31 (5) ◽  
pp. 754-759 ◽  
Author(s):  
Akihiro Nakabayashi ◽  
Naosuke Kamei ◽  
Toru Sunagawa ◽  
Osami Suzuki ◽  
Shingo Ohkawa ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Muscle Injury ◽  
Bioluminescence Imaging ◽  
Skeletal Muscle Injury ◽  
Injury Model ◽  
In Vivo Bioluminescence Imaging

Repair of Traumatic Skeletal Muscle Injury with Bone-Marrow-Derived Mesenchymal Stem Cells Seeded on Extracellular Matrix

2010 ◽  
Vol 16 (9) ◽  
pp. 2871-2881 ◽  
Author(s):  
Edward K. Merritt ◽  
Megan V. Cannon ◽  
David W. Hammers ◽  
Long N. Le ◽  
Rohit Gokhale ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Muscle Injury ◽  
Skeletal Muscle Injury

Immediate and delayed transplantation of mesenchymal stem cells improve muscle force after skeletal muscle injury in rats

2012 ◽  
Vol 6 (S3) ◽  
pp. s60-s67 ◽  
Author(s):  
Tobias Winkler ◽  
Philipp von Roth ◽  
Piotr Radojewski ◽  
Alexander Urbanski ◽  
Sebastian Hahn ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Muscle Injury ◽  
Muscle Force ◽  
Skeletal Muscle Injury

scholarly journals In Vivo Bioluminescence Imaging of Transplanted Mesenchymal Stem Cells as a Potential Source for Pancreatic Regeneration

2014 ◽  
Vol 13 (8) ◽  
pp. 7290.2014.00023 ◽  
Author(s):  
Song Lee ◽  
Hyewon Youn ◽  
Taemoon Chung ◽  
Do Won Hwang ◽  
So Won Oh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bioluminescence Imaging ◽  
Pancreatic Regeneration ◽  
Potential Source ◽  
In Vivo Bioluminescence Imaging

scholarly journals In vivo bioluminescence imaging study to monitor ectopic bone formation by luciferase gene marked mesenchymal stem cells

2008 ◽  
Vol 26 (7) ◽  
pp. 901-909 ◽  
Author(s):  
Cristina Olivo ◽  
Jacqueline Alblas ◽  
Vivienne Verweij ◽  
Anton-Jan Van Zonneveld ◽  
Wouter J. A. Dhert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bioluminescence Imaging ◽  
Imaging Study ◽  
Ectopic Bone Formation ◽  
Luciferase Gene ◽  
Ectopic Bone ◽  
In Vivo Bioluminescence Imaging

scholarly journals In VivoTracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Juan Cao ◽  
Shike Hou ◽  
Hui Ding ◽  
Ziquan Liu ◽  
Meijuan Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bioluminescence Imaging ◽  
Ex Vivo ◽  
Allogeneic Bone Marrow ◽  
Therapeutic Effects ◽  
Allogeneic Bone ◽  
Marrow Mesenchymal Stem Cells

Recently, mesenchymal stem cells (MSCs) are increasingly used as a panacea for multiple types of disease short of effective treatment. Dozens of clinical trials published demonstrated strikingly positive therapeutic effects of MSCs. However, as a specific agent, little research has focused on the dynamic distribution of MSCs afterin vivoadministration. In this study, we track systemically transplanted allogeneic bone marrow mesenchymal stem cells (BMSCs) in normal rats through bioluminescence imaging (BLI) in real time.Ex vivoorgan imaging, immunohistochemistry (IHC), and RT-PCR were conducted to verify the histological distribution of BMSCs. Our results showed that BMSCs home to the dorsal skin apart from the lungs and kidneys after tail vein injection and could not be detected 14 days later. Allogeneic BMSCs mainly appeared not at the parenchymatous organs but at the subepidermal connective tissue and adipose tissue in healthy rats. There were no significant MSCs-related adverse effects except for transient decrease in neutrophils. These findings will provide experimental evidences for a better understanding of the biocharacteristics of BMSCs.

scholarly journals Human Umbilical Cord Mesenchymal Stem Cells Extricate Bupivacaine-Impaired Skeletal Muscle Function via Mitigating Neutrophil-Mediated Acute Inflammation and Protecting against Fibrosis

2019 ◽  
Vol 20 (17) ◽  
pp. 4312 ◽  
Author(s):  
Wen-Hong Su ◽  
Ching-Jen Wang ◽  
Hung-Chun Fu ◽  
Chien-Ming Sheng ◽  
Ching-Chin Tsai ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Umbilical Cord ◽  
Muscle Injury ◽  
Acute Inflammation ◽  
Transforming Growth Factor ◽  
Skeletal Muscle Injury ◽  
Tgf Β1

Skeletal muscle injury presents a challenging traumatological dilemma, and current therapeutic options remain mediocre. This study was designed to delineate if engraftment of mesenchymal stem cells derived from umbilical cord Wharton’s jelly (uMSCs) could aid in skeletal muscle healing and persuasive molecular mechanisms. We established a skeletal muscle injury model by injection of myotoxin bupivacaine (BPVC) into quadriceps muscles of C57BL/6 mice. Post BPVC injection, neutrophils, the first host defensive line, rapidly invaded injured muscle and induced acute inflammation. Engrafted uMSCs effectively abolished neutrophil infiltration and activation, and diminished neutrophil chemotaxis, including Complement component 5a (C5a), Keratinocyte chemoattractant (KC), Macrophage inflammatory protein (MIP)-2, LPS-induced CXC chemokine (LIX), Fractalkine, Leukotriene B4 (LTB4), and Interferon-γ, as determined using a Quantibody Mouse Cytokine Array assay. Subsequently, uMSCs noticeably prevented BPVC-accelerated collagen deposition and fibrosis, measured by Masson’s trichrome staining. Remarkably, uMSCs attenuated BPVC-induced Transforming growth factor (TGF)-β1 expression, a master regulator of fibrosis. Engrafted uMSCs attenuated TGF-β1 transmitting through interrupting the canonical Sma- And Mad-Related Protein (Smad)2/3 dependent pathway and noncanonical Smad-independent Transforming growth factor beta-activated kinase (TAK)-1/p38 mitogen-activated protein kinases signaling. The uMSCs abrogated TGF-β1-induced fibrosis by reducing extracellular matrix components including fibronectin-1, collagen (COL) 1A1, and COL10A1. Most importantly, uMSCs modestly extricated BPVC-impaired gait functions, determined using CatWalk™ XT gait analysis. This work provides several innovative insights into and molecular bases for employing uMSCs to execute therapeutic potential through the elimination of neutrophil-mediated acute inflammation toward protecting against fibrosis, thereby rescuing functional impairments post injury.

scholarly journals Comparative Study on Bone Marrow-Versus Adipose-Derived Stem Cells on Regeneration and Re-Innervation of Skeletal Muscle Injury in Wistar Rats

2020 ◽  
Vol 17 (6) ◽  
pp. 887-900
Author(s):  
Manal H. Moussa ◽  
Ghada G. Hamam ◽  
Asmaa E. Abd Elaziz ◽  
Marwa A. Rahoma ◽  
Abeer A. Abd El Samad ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Comparative Study ◽  
Muscle Injury ◽  
Wistar Rats ◽  
Adipose Derived Stem Cells ◽  
Skeletal Muscle Injury

scholarly journals Stem Cells for the Treatment of Skeletal Muscle Injury

2009 ◽  
Vol 28 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Andres J. Quintero ◽  
Vonda J. Wright ◽  
Freddie H. Fu ◽  
Johnny Huard
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Muscle Injury ◽  
Skeletal Muscle Injury
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