The effects of hydrostatic pressure on matrix synthesis in articular cartilage

1991 ◽  
Vol 9 (1) ◽  
pp. 1-10 ◽  
Author(s):  
A. C. Hall ◽  
J. P. G. Urban ◽  
K. A. Gehl
Keyword(s):  
Articular Cartilage ◽  
Hydrostatic Pressure ◽  
Matrix Synthesis

scholarly journals Optimization of Extracellular Matrix Synthesis and Accumulation by Human Articular Chondrocytes in 3-Dimensional Construct with Repetitive Hydrostatic Pressure

Cartilage ◽  
2017 ◽  
Vol 9 (2) ◽  
pp. 192-201 ◽  
Author(s):  
Takahiro Ogura ◽  
Akihiro Tsuchiya ◽  
Tom Minas ◽  
Shuichi Mizuno
Keyword(s):  
Extracellular Matrix ◽  
Hydrostatic Pressure ◽  
Articular Chondrocytes ◽  
Core Protein ◽  
Collagen Gel ◽  
Human Articular Chondrocytes ◽  
Histological Evaluation ◽  
Matrix Synthesis ◽  
Gene Expressions ◽  
Significant Difference

Objective The effects of hydrostatic pressure (HP) on the matrix synthesis by human articular chondrocytes have been reported elsewhere. In order to optimize the production of extracellular matrix, we aimed to clarify the effects of repetitive HP on metabolic function by human articular chondrocytes. Design The human articular chondrocytes were expanded and embedded within a collagen gel/sponge scaffold. We incubated these constructs with and without HP followed by atmospheric pressure (AP) and repeated the second HP followed by AP over 14 days. Genomic, biochemical, and histological evaluation were performed to compare the effects of each regimen on the constructs. Results The gene expressions of collagen type II and aggrecan core protein were significantly upregulated with repetitive HP regimens compared with a single HP or AP by 14 days ( P < 0.01 or 0.05). Matrix metalloptoteinase-13 (MMP-13) in AP was upregulated significantly compared to other HP regimens at day 14 ( P < 0.01). No significant difference was observed in tissue inhibitor of metalloproteinases-II. Immunohistology demonstrated that application of HP (both repetitive and single) promoted the accumulation of specific extracellular matrix and reduced a MMP-13. A single regimen of HP followed by AP significantly increased the amount of sulfated glycosaminoglycan than that of the AP, whereas repetitive HP remained similar level of that of the AP. Conclusions Repetitive HP had a greater effect on anabolic activity by chondrocytes than a single HP regimen, which will be advantageous for producing a matrix-rich cell construct.

scholarly journals Regulation of Cell Behavior by Hydrostatic Pressure

2019 ◽  
Vol 71 (4) ◽  
Author(s):  
Shaobao Liu ◽  
Ru Tao ◽  
Ming Wang ◽  
Jin Tian ◽  
Guy M. Genin ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Hydrostatic Pressure ◽  
Cell Behavior ◽  
Cell Behaviors ◽  
Recent Advances ◽  
Tissue Systems ◽  
The Brain

Hydrostatic pressure (HP) regulates diverse cell behaviors including differentiation, migration, apoptosis, and proliferation. Abnormal HP is associated with pathologies including glaucoma and hypertensive fibrotic remodeling. In this review, recent advances in quantifying and predicting how cells respond to HP across several tissue systems are presented, including tissues of the brain, eye, vasculature and bladder, as well as articular cartilage. Finally, some promising directions on the study of cell behaviors regulated by HP are proposed.

Combination of reduced oxygen tension and intermittent hydrostatic pressure: a useful tool in articular cartilage tissue engineering

2001 ◽  
Vol 34 (7) ◽  
pp. 941-949 ◽  
Author(s):  
Ute Hansen ◽  
Michael Schünke ◽  
Christian Domm ◽  
Niki Ioannidis ◽  
Joachim Hassenpflug ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Hydrostatic Pressure ◽  
Oxygen Tension ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue

Effects of Hydrostatic Pressure on Matrix Synthesis and Matrix Metalloproteinase Production in the Human Lumbar Intervertebral Disc

Spine ◽  
1997 ◽  
Vol 22 (10) ◽  
pp. 1085-1091 ◽  
Author(s):  
Tadashi Handa ◽  
Hirokazu Ishihara ◽  
Hiroshi Ohshima ◽  
Ryusuke Osada ◽  
Haruo Tsuji ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
Matrix Metalloproteinase ◽  
Intervertebral Disc ◽  
Matrix Synthesis ◽  
Lumbar Intervertebral Disc
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