scholarly journals Real‐world application of the Xpert® HBV viral load assay on serum and dried blood spots

Author(s):  
Kathy Jackson ◽  
Rosemary Tekoaua ◽  
Xin Li ◽  
Stephen Locarnini
2011 ◽  
Vol 50 (3) ◽  
pp. 569-572 ◽  
Author(s):  
M. Arredondo ◽  
C. Garrido ◽  
N. Parkin ◽  
N. Zahonero ◽  
S. Bertagnolio ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126878 ◽  
Author(s):  
Sue Napierala Mavedzenge ◽  
Calum Davey ◽  
Tarisai Chirenje ◽  
Phyllis Mushati ◽  
Sibongile Mtetwa ◽  
...  

2020 ◽  
Vol 59 (1) ◽  
pp. e02303-20
Author(s):  
Dana N. Raugi ◽  
Robert S. Nixon ◽  
Sally Leong ◽  
Khadim Faye ◽  
Jean Phillipe Diatta ◽  
...  

ABSTRACTThe treatment of HIV-2 in resource-limited settings (RLS) is complicated by the limited availability of HIV-2-active antiretroviral drugs and inadequate access to HIV-2 viral load and drug resistance testing. Dried blood spots (DBS)-based drug resistance testing, widely studied for HIV-1, has not been reported for HIV-2 and could present an opportunity to improve care for HIV-2-infected individuals. We selected 150 DBS specimens from ongoing studies of antiretroviral therapy (ART) for HIV-2 infection in Senegal and subjected them to genotypic drug resistance testing. Total nucleic acid was extracted from DBS, reverse transcribed, PCR amplified, and analyzed by population-based Sanger sequencing, and major drug resistance-associated mutations (RAM) were identified. Parallel samples from plasma and peripheral blood mononuclear cells (PBMC) were also genotyped. We obtained 58 protease/reverse transcriptase genotypes. Plasma viral load was significantly correlated with genotyping success (P < 0.001); DBS samples with corresponding plasma viral load >250 copies/ml had a success rate of 86.8%. In paired DBS-plasma genotypes, 83.8% of RAM found in plasma were also found in DBS, and replicate DBS genotyping revealed that a single test detected 86.7% of known RAM. These findings demonstrate that DBS-based genotypic drug resistance testing for HIV-2 is feasible and can be deployed in RLS with limited infrastructure.


PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0124748 ◽  
Author(s):  
Sarah E. Rutstein ◽  
Mina C. Hosseinipour ◽  
Deborah Kamwendo ◽  
Alice Soko ◽  
Memory Mkandawire ◽  
...  

2016 ◽  
Vol 10 (35) ◽  
pp. 1439-1443
Author(s):  
Dagnra Anoumou ◽  
Salou Mounerou ◽  
Ehlan Amivi ◽  
Konou Ahouefa ◽  
Ouro-Medeli Alassane ◽  
...  

2015 ◽  
Vol 72 ◽  
pp. 20-24 ◽  
Author(s):  
Michael J. Vinikoor ◽  
Samuel Zürcher ◽  
Kalo Musukuma ◽  
Obert Kachuwaire ◽  
Andri Rauch ◽  
...  

2009 ◽  
Vol 49 (6) ◽  
pp. 976-981 ◽  
Author(s):  
Asgeir Johannessen ◽  
Carolina Garrido ◽  
Natalia Zahonero ◽  
Leiv Sandvik ◽  
Ezra Naman ◽  
...  

2018 ◽  
Vol 251 ◽  
pp. 75-79 ◽  
Author(s):  
Emilande Guichet ◽  
Laetitia Serrano ◽  
Christian Laurent ◽  
Sabrina Eymard-Duvernay ◽  
Christopher Kuaban ◽  
...  

2019 ◽  
Vol 49 (1) ◽  
pp. 195-209 ◽  
Author(s):  
Richard A. Teran ◽  
Adam W. Carrico ◽  
Keith J. Horvath ◽  
Martin J. Downing ◽  
Mary Ann Chiasson ◽  
...  

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