The using of gene-oriented SCoT markers in genotyping of the Apple genus (Malus Mill.)

This paper discusses the aspects of optimization of the SCoT genotyping method for representatives of the genus Apple (Malus Mill.), including the orchard apple (Malus domestica). Special attention is paid to the methods of total DNA isolation from apple plant tissue, which is due to the sensitivity of multilocus marker systems to the quality of the nucleic acid preparation. On a sample of total DNA isolated from the leaves of the Golden Delicious cultivar, 18 SCoT markers were tested, from which 4 Malus Mill cultivars and species most promising for genotyping were selected.

A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications

Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB–DA composite. This novel CB–DA composite achieved a capture efficiency of approximately 90% at an Escherichia coli concentration of 106 CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB–DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems.