scholarly journals Performance of dried blood spot (DBS) PUNCHER and dried blood spots to measure HIV-1 viral load

2016 ◽  
Vol 10 (35) ◽  
pp. 1439-1443
Author(s):  
Dagnra Anoumou ◽  
Salou Mounerou ◽  
Ehlan Amivi ◽  
Konou Ahouefa ◽  
Ouro-Medeli Alassane ◽  
...  
Keyword(s):  
Viral Load ◽  
Dried Blood Spots ◽  
Dried Blood Spot ◽  
Blood Spots ◽  
Hiv 1 ◽  
Blood Spot

scholarly journals Development and validation of a LC-MS/MS method for ivermectin quantification in dried blood spots: application to a pharmacokinetic study inTrichuris trichiura-infected adults

2018 ◽  
Vol 10 (24) ◽  
pp. 2901-2909 ◽  
Author(s):  
Jessica D. Schulz ◽  
Anna Neodo ◽  
Jean T. Coulibaly ◽  
Jennifer Keiser
Keyword(s):  
Pharmacokinetic Study ◽  
Dried Blood Spots ◽  
Trichuris Trichiura ◽  
Dried Blood Spot ◽  
Plasma Samples ◽  
Blood Spots ◽  
Development And Validation ◽  
Blood Spot

Ivermectin was quantified in dried blood spot and plasma samples derived fromTrichuris trichiura-infected adults with a validated LC-MS/MS method.

scholarly journals Measurement of total homocysteine in plasma and blood spots using liquid chromatography-tandem mass spectrometry: comparison with the plasma Abbott IMx method

2003 ◽  
Vol 40 (2) ◽  
pp. 161-165 ◽  
Author(s):  
Steven J. McCann ◽  
Scott Gillingwater ◽  
Brian G. Keevil ◽  
Donald P. Cooper ◽  
Michel R. Morris
Keyword(s):  
Sensitivity And Specificity ◽  
Plasma Concentrations ◽  
Dried Blood Spots ◽  
Dried Blood Spot ◽  
Tandem Mass ◽  
Blood Spots ◽  
Plasma Thcy ◽  
Total Homocysteine ◽  
Tandem Mass Spectrometric ◽  
Blood Spot

Background: Current sampling for total homocysteine (tHcy) is problematic, requiring plasma separation within 15 min. The aim of this study was to develop a liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the measurement of tHcy in plasma and dried blood spots and to determine whether the dried blood spot concentration could be used to predict plasma concentrations of tHcy. Methods: LC-MS/MS methodology was optimized to measure tHcy in plasma and dried blood spots. Fifty blood samples collected from heart transplant patients were used to form dried blood spots and for plasma analysis. Plasma tHcy was also measured using the Abbott IMx1 method and values were compared to the tHcy concentrations determined in plasma and dried blood spots using LC-MS/MS methodology. Results: The plasma tHcy LC-MS/MS results compared well with the IMx values: LC-MS/MS=1·18(IMx)-0·44 ( r2=0·915). The within-batch precision ( n =10) of the plasma LC-MS/MS method was < 2·0% at 14·6 and 37·7 µmol/L, respectively; the between-batch precision ( n=10) was 5·0 and 8·0%, respectively, at these concentrations. The method was found to be sensitive down to 1 µmol/L and linear up to at least 100 µmol/L. Dried blood spot LC-MS/MS results were considerably lower than the plasma IMx values ( P < 0·0001): dried blood spot LC-MS/MS=0·33IMx+1·77 ( r2=0·682). The within-batch precision ( n=20) of the dried blood spot LC-MS/MS method was 7·3% and 4·7% at concentrations of 4·0 and 7·9 µmol/L, respectively; the between-batch precision was 12·6% and 7·9% at concentrations of 5·1 and 8·0 µmol/L, respectively. To assess whether dried blood spots are suitable as a screening test to predict plasma tHcy concentrations, arbitary cut-off levels were compared. If it is assumed that a plasma tHcy concentration of >15 µmol/L is raised, a dried blood spot result of >6·8 µmol/L has a sensitivity and specificity in detecting a raised plasma tHcy of 83·3% and 96·2%, respectively, and a positive and negative predictive value of 95% and 86%, respectively, with an efficiency of 90%. Use of a dried blood spot cut-off concentration of 6·2 µmol/L for predicting high plasma tHcy concentrations (above 15 µmol/L) has a sensitivity and specificity of 95·8% and 73·1%, respectively, positive and negative predictive values of 76% and 95%, respectively, and an efficiency of 84%. Conclusions: We have developed a precise and accurate LC-MS/MS method for measuring plasma tHcy concentrations, which uses a small volume of plasma and is suitable for routine use. A satisfactory LC-MS/MS method for the measurement of tHcy in dried blood spots was also developed; this method might be useful in routine screening for raised plasma concentrations of tHcy.

Comparison of different nucleic acid preparation methods to improve specific HIV-1 RNA isolation for viral load testing on dried blood spots

2018 ◽  
Vol 251 ◽  
pp. 75-79 ◽  
Author(s):  
Emilande Guichet ◽  
Laetitia Serrano ◽  
Christian Laurent ◽  
Sabrina Eymard-Duvernay ◽  
Christopher Kuaban ◽  
...  
Keyword(s):  
Viral Load ◽  
Nucleic Acid ◽  
Rna Isolation ◽  
Dried Blood Spots ◽  
Load Testing ◽  
Preparation Methods ◽  
Blood Spots ◽  
Viral Load Testing ◽  
Nucleic Acid Preparation ◽  
Hiv 1

scholarly journals Dried Blood Spots versus Sera for Detection of Rubella Virus-Specific Immunoglobulin M (IgM) and IgG in Samples Collected during a Rubella Outbreak in Peru

2007 ◽  
Vol 14 (11) ◽  
pp. 1522-1525 ◽  
Author(s):  
Rita F. Helfand ◽  
Cesar Cabezas ◽  
Emily Abernathy ◽  
Carlos Castillo-Solorzano ◽  
Ana Cecilia Ortiz ◽  
...  
Keyword(s):  
Filter Paper ◽  
Rubella Virus ◽  
Dried Blood Spots ◽  
Immunoglobulin M ◽  
Dried Blood Spot ◽  
Blood Spots ◽  
Specific Immunoglobulin ◽  
Blood Spot

ABSTRACT Most persons with rubella virus-specific immunoglobulin M (IgM)- or IgG-positive sera tested positive (98% [n = 178] and 99% [n = 221], respectively) using paired filter paper dried blood spot (DBS) samples, provided that DBS indeterminate results were called positive. For persons with IgM- or IgG-negative sera, 97% and 98%, respectively, were negative using DBS.

scholarly journals Evaluation of the performance of Abbott m2000 and Roche COBAS Ampliprep/COBAS Taqman assays for HIV-1 viral load determination using dried blood spots and dried plasma spots in Kenya

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0179316 ◽  
Author(s):  
Clement Zeh ◽  
Kenneth Ndiege ◽  
Seth Inzaule ◽  
Rebecca Achieng ◽  
John Williamson ◽  
...  
Keyword(s):  
Viral Load ◽  
Dried Blood Spots ◽  
Cobas Taqman ◽  
Blood Spots ◽  
Viral Load Determination ◽  
Hiv 1 ◽  
Roche Cobas

scholarly journals Measures of viral load using Abbott RealTime HIV-1 Assay on venous and fingerstick dried blood spots from provider-collected specimens in Malawian District Hospitals

2014 ◽  
Vol 60 (4) ◽  
pp. 392-398 ◽  
Author(s):  
Sarah E. Rutstein ◽  
Deborah Kamwendo ◽  
Lebah Lugali ◽  
Isaac Thengolose ◽  
Gerald Tegha ◽  
...  
Keyword(s):  
Viral Load ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
District Hospitals ◽  
Hiv 1

scholarly journals Comparison of HIV-1 RNA Measurements Obtained by Using Plasma and Dried Blood Spots in the Automated Abbott Real-Time Viral Load Assay

2011 ◽  
Vol 50 (3) ◽  
pp. 569-572 ◽  
Author(s):  
M. Arredondo ◽  
C. Garrido ◽  
N. Parkin ◽  
N. Zahonero ◽  
S. Bertagnolio ◽  
...  
Keyword(s):  
Viral Load ◽  
Real Time ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Viral Load Assay ◽  
Hiv 1
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