High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

2019 ◽  
Vol 92 (2) ◽  
pp. 174-186 ◽  
Author(s):  
Ratana Tacharoenmuang ◽  
Satoshi Komoto ◽  
Ratigorn Guntapong ◽  
Sompong Upachai ◽  
Phakapun Singchai ◽  
...  
2014 ◽  
Vol 143 (2) ◽  
pp. 316-324 ◽  
Author(s):  
C. F. MANSO ◽  
J. L. ROMALDE

SUMMARYThe high incidence of norovirus (NoV) infections seems to be related to the emergence of new variants that evolved by genetic drift of the capsid gene. In this work, that represents a first effort to describe the molecular epidemiology of NoV in the northwest of Spain, a total of eight different NoV genotypes (GII.1, GII.3, GII.4, GII.6, GII.7, GII.12, GII.13, GII.14) were detected. The major genotypes observed were GII.4 (45·42%) and GII.14 (34·9%), being detected in all age groups. In addition, and although most of GII.4 sequences belonged to 2006b (7·2%) and 2010 (50·35%) variants, the presence of new NoV variants was observed. Phylogenetic analysis revealed that a high number of GII.4 sequences (35·24%) could be assigned to the newly emerging Sydney 2012 variant, even during late 2010. The high prevalence of NoV GII.14 observed in this study may indicate the emergence of this genotype in Spain.


2019 ◽  
Vol 63 (03) ◽  
pp. 328-332
Author(s):  
Y. LI ◽  
P. ZHANG ◽  
X. WU ◽  
D. WEN ◽  
L. JI ◽  
...  

2011 ◽  
Vol 6 (02) ◽  
pp. 148-155 ◽  
Author(s):  
Christabel C Enweronu-Laryea ◽  
Kwamena W.C. Sagoe ◽  
Hope Glover-Addy ◽  
Richard H Asmah ◽  
Julius A Mingle ◽  
...  

Introduction: Vaccination is the most effective preventive strategy against rotavirus disease. Regional differences in prevalent rotavirus genotypes may affect vaccine efficacy. Pre-vaccine surveillance for burden of rotavirus disease, prevalent rotavirus genotypes, and association between rotavirus disease and intussusceptions helps in monitoring the impact of vaccination. Methodology: A prospective study was conducted from January 2008 to December 2009 in children younger than five years hospitalized for longer than 24 hours with acute gastroenteritis. Data on confirmed cases of intussusception were collected retrospectively. Stools were tested by enzyme immunoassay, reverse-transcriptase polymerase chain reaction and nucleotide sequencing.  Results: Acute gastroenteritis (AGE) caused 13.1% (2,147/16,348) of hospitalizations among children under five years. Stools were tested for 50.2% (1077/2147) of AGE cases. Of these, 49% (528/1077) were rotavirus positive. Rotavirus gastroenteritis, non-rotavirus gastroenteritis, and intussusceptions were most prevalent in children under 15 months [80.3%, 74% and 91% respectively]. Rotavirus was detected from more than 60% of acute gastroenteritis cases during peak months. The prevalence of intussusception showed no seasonal pattern. The peak ages of six to twelve months for acute gastroenteritis and five to eight months for intussusception overlapped. G1, G2 and mixed G/P genotypes were common in the isolated rotaviruses. Conclusion: Rotavirus gastroenteritis causes significant morbidity in children younger than five years of age in Ghana. Although the peak age of rotavirus gastroenteritis and intussusceptions overlapped, there was no seasonal correlation between them. The high prevalence of mixed G/P genotypes in Ghanaian children may affect the effectiveness of vaccination.


2019 ◽  
Vol 91 (5) ◽  
pp. 744-750 ◽  
Author(s):  
Rodrigo Pratte-Santos ◽  
Marize Pereira Miagostovich ◽  
Túlio Machado Fumian ◽  
Ethel Leonor Maciel ◽  
Sara Angelino Martins ◽  
...  

2011 ◽  
Vol 52 (3) ◽  
pp. 251-253 ◽  
Author(s):  
Yu Jin ◽  
Wei-xia Cheng ◽  
Zi-qian Xu ◽  
Na Liu ◽  
Jie-mei Yu ◽  
...  

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9645
Author(s):  
Kitsakorn Rojjanadumrongkul ◽  
Kattareeya Kumthip ◽  
Pattara Khamrin ◽  
Nuthapong Ukarapol ◽  
Hiroshi Ushijima ◽  
...  

Background Infection with viruses especially rotavirus, norovirus, astrovirus, and adenovirus has been known to be a major cause of acute gastroenteritis in children under 5 years of age globally, particularly in developing countries. Also, some genotypes of enteroviruses (EVs) have been reported to be associated with gastroenteritis. This study is aimed to investigate the prevalence and genotype diversity of EV in children admitted to hospitals with acute gastroenteritis. Methods A total of 1,736 fecal specimens were collected from children hospitalized with diarrhea in Chiang Mai, Thailand from 2015 to 2018. All specimens were tested for the presence of EV by RT-PCR of the 5′ untranslated region. The genotypes of EV were further identified by nucleotide sequencing and phylogenetic analysis of the viral protein 1 (VP1) gene. Results EV was detected in 154 out of 1,736 specimens (8.9%) throughout the study period. The prevalence of EV detected in 2015, 2016, 2017, and 2018 was 7.2%, 9.0%, 11.2%, and 8.6%, respectively. EV was detected all year round with a high prevalence during rainy season in Thailand. Overall, 37 genotypes of EV were identified in this study. Among these, coxsackievirus (CV)-A24 and CV-B5 (7.5% each), and EV-C96 (6.8%) were the common genotypes detected. Conclusion This study demonstrates the prevalence, seasonal distribution, and genotype diversity of EV circulating in children hospitalized with acute gastroenteritis in Chiang Mai, Thailand during the period 2015 to 2018.


2019 ◽  
Vol 24 (12) ◽  
pp. 1427-1433 ◽  
Author(s):  
Vivaldie Mikounou Louya ◽  
Boris Nguekeng Tsague ◽  
Francine Ntoumi ◽  
Christevy Vouvoungui ◽  
Simon Charles Kobawila

Author(s):  
Marcelle Silva-Sales ◽  
Mariana Seglia Caldas ◽  
Julia Monassa Fioretti ◽  
Monica Simoes Rocha ◽  
Tulio Machado Fumian ◽  
...  

Gastroenteric viruses are important pathogens related to cases of acute gastroenteritis, affecting millions of people worldwide with a major impact on children under five in developing countries. The introduction of metagenomic approach techniques in the 2000s has allowed the description of new viruses, among them Salivirus, which has been associated worldwide with cases of diarrhea. This study aimed to detect salivirus in raw sewage samples from a wastewater treatment plant (WWTP) collected between June 2013 and May 2014 in Rio de Janeiro, Brazil. Fifty-two samples collected weekly were tested by using a real-time quantitative PCR (qPCR).  Salivirus genome was detected in 71.1% (37/52) of the samples, with viral concentration ranging from 7.56 x 104 to 7.20 x 106 genomic copies per liter. Higher viral loads were detected in the summer and fall of 2014, although these data were not sufficient to infer seasonality for this virus. The high prevalence of salivirus in sewage samples highlights the importance of viral research in wastewater to generate data on salivirus circulation, increasing understanding regarding its dissemination in the population.KEY WORDS: Wastewater; viral detection; salivirus; Brazil; raw sewage; qPCR.


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