scholarly journals Three-dimensional method for comparing in vivo interventional MR images of thermally ablated tissue with tissue response

2003 ◽  
Vol 18 (1) ◽  
pp. 90-102 ◽  
Author(s):  
Michael S. Breen ◽  
Tanya L. Lancaster ◽  
Roee S. Lazebnik ◽  
Sherif G. Nour ◽  
Jonathan S. Lewin ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Tissue Response ◽  
Mr Images ◽  
Interventional Mr

Three-dimensional correlation of MR images to muscle tissue response for interventional MRI thermal ablation

2001 ◽  
Author(s):  
Michael S. Breen ◽  
Tanya L. Lancaster ◽  
Roee S. Lazebnik ◽  
Andrik J. Ashcroft ◽  
Sherif Gamal Nour ◽  
...  
Keyword(s):  
Muscle Tissue ◽  
Thermal Ablation ◽  
Three Dimensional ◽  
Interventional Mri ◽  
Tissue Response ◽  
Mr Images

Three-dimensional comparison of interventional MR radiofrequency ablation images with tissue response†

2004 ◽  
Vol 9 (5) ◽  
pp. 185-191 ◽  
Author(s):  
Michael Breen ◽  
Roee Lazebnik ◽  
Sherif Nour ◽  
Jonathan Lewin ◽  
David Wilson
Keyword(s):  
Radiofrequency Ablation ◽  
Three Dimensional ◽  
Tissue Response ◽  
Interventional Mr

scholarly journals Ex Vivo MR Histology and Cytometric Feature Mapping Connect Three-dimensional in Vivo MR Images to Two-dimensional Histopathologic Images of Murine Sarcomas

2021 ◽  
Vol 3 (3) ◽  
pp. e200103
Author(s):  
Stephanie J. Blocker ◽  
James Cook ◽  
Yvonne M. Mowery ◽  
Jeffrey I. Everitt ◽  
Yi Qi ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Mr Images ◽  
Feature Mapping

High-resolution imaging reveals a limit in spatial resolution of blood flow measurements by microspheres

2004 ◽  
Vol 287 (3) ◽  
pp. H1132-H1140 ◽  
Author(s):  
Ulrich K. M. Decking ◽  
Vinay M. Pai ◽  
Eric Bennett ◽  
Joni L. Taylor ◽  
Christian D. Fingas ◽  
...  
Keyword(s):  
Blood Flow ◽  
Spatial Resolution ◽  
Three Dimensional ◽  
Epifluorescence Microscopy ◽  
Canine Heart ◽  
Mr Images ◽  
Deposition Pattern ◽  
Flow Measurements

Density of 15-μm microspheres after left atrial application is the standard measure of regional perfusion. In the heart, substantial differences in microsphere density are seen at spatial resolutions <5 ml, implying perfusion heterogeneity. Microsphere deposition imaging permits a superior evaluation of the distribution pattern. Therefore, fluorescent microspheres (FMS) were applied, FMS deposition in the canine heart was imaged by epifluorescence microscopy in vitro, and the patterns were observed compared with MR images of iron oxide microspheres (IMS) obtained in vivo and in vitro. FMS deposition in myocardial slices revealed the following: 1) a nonrandom distribution, with sequentially applied FMS of different color stacked within the same vessel, 2) general FMS clustering, and 3) rather large areas devoid of FMS ( n = 3). This pattern was also seen in reconstructed three-dimensional images (<1 nl resolution) of FMS distribution ( n = 4). Surprisingly, the deposition pattern of sequentially applied FMS remained virtually identical over 3 days. Augmenting flow by intracoronary adenosine (>2 μM) enhanced local microsphere density, but did not alter the deposition pattern ( n = 3). The nonrandom, temporally stable pattern was quantitatively confirmed by a three-dimensional intermicrosphere distance analysis of sequentially applied FMS. T2-weighted short-axis MR images (2-μl resolution) of IMS revealed similar patterns in vivo and in vitro ( n = 6), as seen with FMS. The observed temporally stable microsphere patterns are not consistent with the notion that microsphere deposition is solely governed by blood flow. We propose that at high spatial resolution (<2 μl) structural aspects of the vascular network dominate microsphere distribution, resulting in the organized patterns observed.

scholarly journals Measuring glomerular number and size in perfused kidneys using MRI

2011 ◽  
Vol 300 (6) ◽  
pp. F1454-F1457 ◽  
Author(s):  
Scott C. Beeman ◽  
Min Zhang ◽  
Lina Gubhaju ◽  
Teresa Wu ◽  
John F. Bertram ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Renal Diseases ◽  
Resonance Imaging ◽  
Mr Images ◽  
Image Processing Algorithm ◽  
Glomerular Volume ◽  
Glomerular Number ◽  
Magnetic Resonance Imaging Mri ◽  
Intrarenal Distribution

The goal of this work was to nondestructively measure glomerular (and thereby nephron) number in the whole kidney. Variations in the number and size of glomeruli have been linked to many renal and systemic diseases. Here, we develop a robust magnetic resonance imaging (MRI) technique based on injection of cationic ferritin (CF) to produce an accurate measurement of number and size of individual glomeruli. High-field (19 Tesla) gradient-echo MR images of perfused rat kidneys after in vivo intravenous injection of CF showed specific labeling of individual glomeruli with CF throughout the kidney. We developed a three-dimensional image-processing algorithm to count every labeled glomerulus. MRI-based counts yielded 33,786 ± 3,753 labeled glomeruli ( n = 5 kidneys). Acid maceration counting of contralateral kidneys yielded an estimate of 30,585 ± 2,053 glomeruli ( n = 6 kidneys). Disector/fractionator stereology counting yielded an estimate of 34,963 glomeruli ( n = 2). MRI-based measurement of apparent glomerular volume of labeled glomeruli was 4.89 × 10−4mm3( n = 5) compared with the average stereological measurement of 4.99 × 10−4mm3( n = 2). The MRI-based technique also yielded the intrarenal distribution of apparent glomerular volume, a measurement previously unobtainable in histology. This work makes it possible to nondestructively measure whole-kidney glomerular number and apparent glomerular volumes to study susceptibility to renal diseases and opens the door to similar in vivo measurements in animals and humans.

scholarly journals Accuracy of 3D Cartilage Models Generated From MR Images Is Dependent on Cartilage Thickness: Laser Scanner Based Validation of In Vivo Cartilage

2009 ◽  
Vol 131 (12) ◽  
Author(s):  
Seungbum Koo ◽  
Nicholas J. Giori ◽  
Garry E. Gold ◽  
Chris O. Dyrby ◽  
Thomas P. Andriacchi
Keyword(s):  
Laser Scanning ◽  
Shape Matching ◽  
Three Dimensional ◽  
3D Models ◽  
Cartilage Thickness ◽  
Mr Images ◽  
Mr Image ◽  
Scanning Method ◽  
Thickness Measurements

Cartilage morphology change is an important biomarker for the progression of osteoarthritis. The purpose of this study was to assess the accuracy of in vivo cartilage thickness measurements from MR image-based 3D cartilage models using a laser scanning method and to test if the accuracy changes with cartilage thickness. Three-dimensional tibial cartilage models were created from MR images (in-plane resolution of 0.55 mm and thickness of 1.5 mm) of osteoarthritic knees of ten patients prior to total knee replacement surgery using a semi-automated B-spline segmentation algorithm. Following surgery, the resected tibial plateaus were laser scanned and made into 3D models. The MR image and laser-scan based models were registered to each other using a shape matching technique. The thicknesses were compared point wise for the overall surface. The linear mixed-effects model was used for statistical test. On average, taking account of individual variations, the thickness measurements in MRI were overestimated in thinner (<2.5 mm) regions. The cartilage thicker than 2.5 mm was accurately predicted in MRI, though the thick cartilage in the central regions was underestimated. The accuracy of thickness measurements in the MRI-derived cartilage models systemically varied according to native cartilage thickness.

Characterization of tissue response to radiofrequency ablation using 3D model-based analysis of interventional MR images

2003 ◽  
Author(s):  
Brent D. Weinberg ◽  
Roee S. Lazebnik ◽  
Michael S. Breen ◽  
Jonathan S. Lewin ◽  
David L. Wilson
Keyword(s):  
Radiofrequency Ablation ◽  
3D Model ◽  
Tissue Response ◽  
Mr Images ◽  
Model Based ◽  
Interventional Mr ◽  
Model Based Analysis

The Effects of the Surface Topography of Micromachined Titanium Substrata on Cell Behavior in Vitro and in Vivo

1999 ◽  
Vol 121 (1) ◽  
pp. 49-57 ◽  
Author(s):  
D. M. Brunette ◽  
B. Chehroudi
Keyword(s):  
Surface Topography ◽  
Fibrous Tissue ◽  
Three Dimensional ◽  
Tissue Response ◽  
Cell Behavior ◽  
Tissue Organization ◽  
Dimensional Reconstruction ◽  
Microfabrication Techniques

Surface properties, including topography and chemistry, are of prime importance in establishing the response of tissues to biomaterials. Microfabrication techniques have enabled the production of precisely controlled surface topographies that have been used as substrata for cells in culture and on devices implanted in vivo. This article reviews aspects of cell behavior involved in tissue response to implants with an emphasis on the effects of topography. Microfabricated grooved surfaces produce orientation and directed locomotion of epithelial cells in vitro and can inhibit epithelial downgrowth on implants. The effects depend on the groove dimensions and they are modified by epithelial cell–cell interactions. Fibroblasts similarly exhibit contact guidance on grooved surfaces, but fibroblast shape in vitro differs markedly from that found in vivo. Surface topography is important in establishing tissue organization adjacent to implants, with smooth surfaces generally being associated with fibrous tissue encapsulation. Grooved topographies appear to have promise in reducing encapsulation in the short term, but additional studies employing three-dimensional reconstruction and diverse topographies are needed to understand better the process of connective-tissue organization adjacent to implants. Microfabricated surfaces can increase the frequency of mineralized bone-like tissue nodules adjacent to subcutaneously implanted surfaces in rats. Orientation of these nodules with grooves occurs both in culture and on implants. Detailed comparisons of cell behavior on micromachined substrata in vitro and in vivo are difficult because of the number and complexity of factors, such as population density and micromotion, that can differ between these conditions.

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