scholarly journals Coupling tumor necrosis factor‐related apoptosis‐inducing ligand to iron oxide nanoparticles increases its apoptotic activity on HCT116 and HepG2 malignant cells: effect of magnetic core size

2019 ◽  
Vol 4 (1) ◽  
pp. 34-50 ◽  
Author(s):  
Hanene Belkahla ◽  
Amranul Haque ◽  
Alexander Revzin ◽  
Tijani Gharbi ◽  
Andrei Alexandru Constantinescu ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Tumor Necrosis Factor ◽  
Iron Oxide Nanoparticles ◽  
Tumor Necrosis ◽  
Magnetic Core ◽  
Oxide Nanoparticles ◽  
Malignant Cells ◽  
Core Size ◽  
Apoptotic Activity ◽  
Necrosis Factor

Research on Mechanism of Superparamagnetic Iron Oxide Nanoparticles in Activating Endoplasmic Reticulum and Prompting Apoptosis of Liver Cells Through Mediation of Tumor Necrosis Factor-α/Tumor Necrosis Factor Receptor 1 (TNF-α/TNFR1) Pathway

2021 ◽  
Vol 17 (12) ◽  
pp. 2413-2419
Author(s):  
Liyang Zhou ◽  
Qin He ◽  
Xiaoàn Yang ◽  
Shuo Zheng ◽  
Xueting Ou
Keyword(s):  
Endoplasmic Reticulum ◽  
Iron Oxide ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Caspase 3 ◽  
Superparamagnetic Iron Oxide ◽  
The Other ◽  
Oxide Nanoparticles ◽  
High Concentration ◽  
Necrosis Factor

The aim of this study was to assess mechanism of superparamagnetic iron oxide nanoparticles (SPIO-NPs) in activating endoplasmic reticulum (ER) and prompting apoptosis of liver cells through mediating the TNF-α/TNFR1 pathway. The SPIO-NPs were prepared and identified, and HegG2 cells were cultivated in vitro, and their apoptosis was detected. The specific pathogen-free (SPF)-grade rats were divided into several groups; which included blank group, low concentration group, high concentration group and control group. The enzymatic activity of Caspase-3 in liver tissue was tested, and expressions of Caspase-3, Bax, Bcl-2, TNF-α, p-TNFR1, IRE1α, and eIF2α were tested. The size of prepared SPIO-NPs was 7.5 nm and there was no coagulation. There was good dispersity and electric potential, and appearance was stable. The apoptotic rate in the high concentration group was notably higher than in the other groups. There was notable inflammatory cell infiltration in the high concentration group, where quantity of apoptosis was highest. The quantity of apoptosis and fluorocyte in the high concentration group were notably higher than in the other groups. Moreover, there were over expressions of Caspase-3, Bax, Caspase-3, p-TNFR1, IRE1α, and eIF2α in the high concentration group while the expression of TNF-α was lowest. The apoptosis of HegG2 cells was prompted by SPIO-NPs, and quantity of apoptosis was increased with increased adopted concentration. The active expression of p-TNFR1, IRE1α, and eIF2α could be prompted to reduce the expression of TNF-α and increase the expression of Caspase-3 and Bax for prompting the apoptosis.

scholarly journals Effects of core size and PEG coating layer of iron oxide nanoparticles on the distribution and metabolism in mice

2018 ◽  
Vol Volume 13 ◽  
pp. 5719-5731 ◽  
Author(s):  
Weiming Xue ◽  
Yanyan Liu ◽  
Na Zhang ◽  
Youdong Yao ◽  
Pei Ma ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Coating Layer ◽  
Oxide Nanoparticles ◽  
Core Size ◽  
Peg Coating

scholarly journals Tumor necrosis factor/cachectin and lymphotoxin gene expression in lymph nodes from lymphoma patients

Blood ◽  
1990 ◽  
Vol 75 (4) ◽  
pp. 958-962
Author(s):  
AP Sappino ◽  
W Seelentag ◽  
MF Pelte ◽  
P Alberto ◽  
P Vassalli
Keyword(s):  
Gene Expression ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Growth Control ◽  
Low Grade ◽  
Mrna Levels ◽  
Malignant Cells ◽  
Systemic Symptoms ◽  
Tissue Specimens ◽  
Necrosis Factor

We investigated the mRNA content for tumor necrosis factor (TNF)/cachectin and lymphotoxin (LT) in tumoral tissues of a prospective series of 35 non-Hodgkin's (NHL) and 23 Hodgkin's (HL) lymphomas, to assess their postulated contribution to systemic symptoms. Total RNAs were extracted from diagnostic tissue specimens and submitted to Northern blot analysis, using specific TNF and LT cRNA probes. High amounts of TNF mRNA were found exclusively in NHL (12/35). The majority (9/12) of these were low grade B-cell NHL, which contained a uniform population of malignant cells. In contrast, abundant LT mRNA production was detected in most HL (21/23) and in 19 of 35 NHL. The highest LT mRNA levels were observed in high grade NHL and in lymphocytic predominant subtypes of HL specimens. A significant correlation was found between TNF/cachectin and LT gene expression in NHL and the presence of constitutional symptoms. The biologic and prognostic implications of these preliminary findings are presently unknown, but they demonstrate that lymphoma tissues sharing common histologic features are highly heterogeneous in their ability to synthesize cytokines susceptible to playing a role in the growth control of malignant cells. These results suggest that the evaluation of TNF/cachectin and LT production in lymphomas may help to elucidate the mechanisms of tumoral fever and cachexia.

scholarly journals Tumor necrosis factor/cachectin and lymphotoxin gene expression in lymph nodes from lymphoma patients

Blood ◽  
1990 ◽  
Vol 75 (4) ◽  
pp. 958-962 ◽  
Author(s):  
AP Sappino ◽  
W Seelentag ◽  
MF Pelte ◽  
P Alberto ◽  
P Vassalli
Keyword(s):  
Gene Expression ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Growth Control ◽  
Low Grade ◽  
Mrna Levels ◽  
Malignant Cells ◽  
Systemic Symptoms ◽  
Tissue Specimens ◽  
Necrosis Factor

Abstract We investigated the mRNA content for tumor necrosis factor (TNF)/cachectin and lymphotoxin (LT) in tumoral tissues of a prospective series of 35 non-Hodgkin's (NHL) and 23 Hodgkin's (HL) lymphomas, to assess their postulated contribution to systemic symptoms. Total RNAs were extracted from diagnostic tissue specimens and submitted to Northern blot analysis, using specific TNF and LT cRNA probes. High amounts of TNF mRNA were found exclusively in NHL (12/35). The majority (9/12) of these were low grade B-cell NHL, which contained a uniform population of malignant cells. In contrast, abundant LT mRNA production was detected in most HL (21/23) and in 19 of 35 NHL. The highest LT mRNA levels were observed in high grade NHL and in lymphocytic predominant subtypes of HL specimens. A significant correlation was found between TNF/cachectin and LT gene expression in NHL and the presence of constitutional symptoms. The biologic and prognostic implications of these preliminary findings are presently unknown, but they demonstrate that lymphoma tissues sharing common histologic features are highly heterogeneous in their ability to synthesize cytokines susceptible to playing a role in the growth control of malignant cells. These results suggest that the evaluation of TNF/cachectin and LT production in lymphomas may help to elucidate the mechanisms of tumoral fever and cachexia.

scholarly journals Albumin-Coated Single-Core Iron Oxide Nanoparticles for Enhanced Molecular Magnetic Imaging (MRI/MPI)

2021 ◽  
Vol 22 (12) ◽  
pp. 6235
Author(s):  
Abdulkader Baki ◽  
Amani Remmo ◽  
Norbert Löwa ◽  
Frank Wiekhorst ◽  
Regina Bleul
Keyword(s):  
Iron Oxide ◽  
Magnetic Resonance ◽  
Contrast Agents ◽  
Iron Oxide Nanoparticles ◽  
Magnetic Particle ◽  
Colloidal Stability ◽  
Simple Procedure ◽  
Oxide Nanoparticles ◽  
Core Size ◽  
Magnetic Iron Oxide Nanoparticles

Colloidal stability of magnetic iron oxide nanoparticles (MNP) in physiological environments is crucial for their (bio)medical application. MNP are potential contrast agents for different imaging modalities such as magnetic resonance imaging (MRI) and magnetic particle imaging (MPI). Applied as a hybrid method (MRI/MPI), these are valuable tools for molecular imaging. Continuously synthesized and in-situ stabilized single-core MNP were further modified by albumin coating. Synthesizing and coating of MNP were carried out in aqueous media without using any organic solvent in a simple procedure. The additional steric stabilization with the biocompatible protein, namely bovine serum albumin (BSA), led to potential contrast agents suitable for multimodal (MRI/MPI) imaging. The colloidal stability of BSA-coated MNP was investigated in different sodium chloride concentrations (50 to 150 mM) in short- and long-term incubation (from two hours to one week) using physiochemical characterization techniques such as transmission electron microscopy (TEM) for core size and differential centrifugal sedimentation (DCS) for hydrodynamic size. Magnetic characterization such as magnetic particle spectroscopy (MPS) and nuclear magnetic resonance (NMR) measurements confirmed the successful surface modification as well as exceptional colloidal stability of the relatively large single-core MNP. For comparison, two commercially available MNP systems were investigated, MNP-clusters, the former liver contrast agent (Resovist), and single-core MNP (SHP-30) manufactured by thermal decomposition. The tailored core size, colloidal stability in a physiological environment, and magnetic performance of our MNP indicate their ability to be used as molecular magnetic contrast agents for MPI and MRI.

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