Morphological and ultrastructural characterization of ionoregulatory cells in the teleost oreochromis niloticus following salinity challenge combining complementary confocal scanning laser microscopy and transmission electron microscopy using a novel pref

2013 ◽  
Vol 76 (10) ◽  
pp. 1016-1024
Author(s):  
Sophie Fridman ◽  
Krishen J. Rana ◽  
James E. Bron
Keyword(s):  
Electron Microscopy ◽  
Oreochromis Niloticus ◽  
Confocal Scanning Laser Microscopy ◽  
Transmission Electron ◽  
Ultrastructural Characterization ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Salinity Challenge

scholarly journals Biofilm Bakteri pada Penderita Rinosinusitis Kronis

2018 ◽  
Vol 1 (2) ◽  
pp. 106
Author(s):  
Yolazenia Yolazenia ◽  
Bestari Jaka Budiman ◽  
Dolly Irfandy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Confocal Scanning Laser Microscopy ◽  
Transmission Electron ◽  
Scanning Laser Microscopy ◽  
Assay Tube ◽  
Confocal Scanning ◽  
Agar Method ◽  
Confocal Scanning Laser ◽  
Scanning Electron

Banyak dilaporkan kegagalan pengobatan pada rinosinusitis kronis (RSK) disebabkan resistensi terhadap antibiotik. Beberapa penelitian menunjukkan bahwa biofilm bakteri berperan penting pada etiologi dan persistensi dari RSK. Penulisan tinjauan pustaka ini adalah untuk mengetahui implikasi biofilm bakteri pada penderita RSK. Rinosinusitis kronis adalah penyakit inflamasi mukosa hidung dan sinus paranasal yang berlangsung dalam waktu lebih dari 12 minggu. Biofilm adalah suatu struktur komunitas sel-sel bakteri yang ditutupi oleh matriks polimer yang dihasilkan sendiri dan menempel pada permukaan. Berbagai penelitian menunjukkan terdapatnya biofilm bakteri pada mukosa sinonasal penderita RSK dan berhubungan dengan resistensi terhadap pengobatan dengan antibiotika. Berbagai pemeriksaan untuk mendeteksi biofilm yaitu Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Confocal Scanning Laser Microscopy (CSLM), modifikasi Calgary Biofilm Device Assay, Tube Method dan Congo Red Agar Method. Beberapa terapi potensial untuk mengatasi biofilm pada RSK sedang berkembang.

scholarly journals The origin of extracellular DNA in bacterial biofilm infections in vivo

2020 ◽  
Vol 78 (2) ◽  
Author(s):  
Maria Alhede ◽  
Morten Alhede ◽  
Klaus Qvortrup ◽  
Kasper Nørskov Kragh ◽  
Peter Østrup Jensen ◽  
...  
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Bacterial Biofilm ◽  
Extracellular Dna ◽  
Confocal Scanning Laser Microscopy ◽  
Transmission Electron ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Pna Fish

ABSTRACT Extracellular DNA (eDNA) plays an important role in both the aggregation of bacteria and in the interaction of the resulting biofilms with polymorphonuclear leukocytes (PMNs) during an inflammatory response. Here, transmission electron and confocal scanning laser microscopy were used to examine the interaction between biofilms of Pseudomonas aeruginosa and PMNs in a murine implant model and in lung tissue from chronically infected cystic fibrosis patients. PNA FISH, DNA staining, labeling of PMN DNA with a thymidine analogue and immunohistochemistry were applied to localize bacteria, eDNA, PMN-derived eDNA, PMN-derived histone H3 (H3), neutrophil elastase (NE) and citrullinated H3 (citH3). Host-derived eDNA was observed surrounding bacterial biofilms but not within the biofilms. H3 localized to the lining of biofilms while NE was found throughout biofilms. CitH3, a marker for neutrophil extracellular traps (NETs) was detected only sporadically indicating that most host-derived eDNA in vivo was not a result of NETosis. Together these observations show that, in these in vivo biofilm infections with P. aeruginosa, the majority of eDNA is found external to the biofilm and derives from the host.

Sign in / Sign up

Export Citation Format

Share Document