Immunolocalization of S100-like protein in the brain of an emerging model organism: Nothobranchius furzeri

2011 ◽  
Vol 75 (4) ◽  
pp. 441-447 ◽  
Author(s):  
Livia D'Angelo ◽  
Paolo De Girolamo ◽  
Alessandro Cellerino ◽  
Eva Terzibasi Tozzini ◽  
Ettore Varricchio ◽  
...  
Keyword(s):  
Model Organism ◽  
Nothobranchius Furzeri ◽  
The Brain

scholarly journals Ontogenetic Pattern Changes of Nucleobindin-2/Nesfatin-1 in the Brain and Intestinal Bulb of the Short Lived African Turquoise Killifish

2019 ◽  
Vol 9 (1) ◽  
pp. 103 ◽  
Author(s):  
Alessia Montesano ◽  
Elena De Felice ◽  
Adele Leggieri ◽  
Antonio Palladino ◽  
Carla Lucini ◽  
...  
Keyword(s):  
Food Intake ◽  
Model Organism ◽  
Aging Research ◽  
Age Related ◽  
Nothobranchius Furzeri ◽  
Food Intake Regulation ◽  
Mammalian Stomach ◽  
Regulate Food Intake ◽  
Intake Regulation ◽  
The Brain

Nesfatin-1 (Nesf-1) was identified as an anorexigenic and well conserved molecule in rodents and fish. While tissue distribution of NUCB2 (Nucleobindin 2)/Nesf-1 is discretely known in vertebrates, reports on ontogenetic expression are scarce. Here, we examine the age-related central and peripheral expression of NUCB2/Nesf-1 in the teleost African turquoise killifish Nothobranchius furzeri, a consolidated model organism for aging research. We focused our analysis on brain areas responsible for the regulation of food intake and the rostral intestinal bulb, which is analogous of the mammalian stomach. We hypothesize that in our model, the stomach equivalent structure is the main source of NUCB2 mRNA, displaying higher expression levels than those observed in the brain, mainly during aging. Remarkably, its expression significantly increased in the rostral intestinal bulb compared to the brain, which is likely due to the typical anorexia of aging. When analyzing the pattern of expression, we confirmed the distribution in diencephalic areas involved in food intake regulation at all age stages. Interestingly, in the rostral bulb, NUCB2 mRNA was localized in the lining epithelium of young and old animals, while Nesf-1 immunoreactive cells were distributed in the submucosae. Taken together, our results represent a useful basis for gaining deeper knowledge regarding the mechanisms that regulate food intake during vertebrate aging.

Neurotrophin Trk receptors in the brain of a teleost fish, Nothobranchius furzeri

2012 ◽  
Vol 75 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Livia D'Angelo ◽  
Paolo de Girolamo ◽  
Alessandro Cellerino ◽  
Eva Terzibasi Tozzini ◽  
Luciana Castaldo ◽  
...  
Keyword(s):  
Teleost Fish ◽  
Trk Receptors ◽  
Nothobranchius Furzeri ◽  
The Brain

scholarly journals Diving into the world of alcohol teratogenesis: a review of zebrafish models of fetal alcohol spectrum disorder

2018 ◽  
Vol 96 (2) ◽  
pp. 88-97 ◽  
Author(s):  
Yohaan Fernandes ◽  
Desire M. Buckley ◽  
Johann K. Eberhart
Keyword(s):  
Fetal Alcohol Spectrum Disorder ◽  
Model Organism ◽  
Spectrum Disorder ◽  
Structural Defects ◽  
Craniofacial Skeleton ◽  
Fetal Alcohol ◽  
Embryonic Exposure ◽  
Fetal Alcohol Spectrum ◽  
The Brain ◽  
Insight Into

The term fetal alcohol spectrum disorder (FASD) refers to the entire suite of deleterious outcomes resulting from embryonic exposure to alcohol. Along with other reviews in this special issue, we provide insight into how animal models, specifically the zebrafish, have informed our understanding of FASD. We first provide a brief introduction to FASD. We discuss the zebrafish as a model organism and its strengths for alcohol research. We detail how zebrafish has been used to model some of the major defects present in FASD. These include behavioral defects, such as social behavior as well as learning and memory, and structural defects, disrupting organs such as the brain, sensory organs, heart, and craniofacial skeleton. We provide insights into how zebrafish research has aided in our understanding of the mechanisms of ethanol teratogenesis. We end by providing some relatively recent advances that zebrafish has provided in characterizing gene-ethanol interactions that may underlie FASD.

scholarly journals Age-Related Alterations in the Behavior and Serotonin-Related Gene mRNA Levels in the Brain of Males and Females of Short-Lived Turquoise Killifish (Nothobranchius furzeri)

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1421
Author(s):  
Valentina S. Evsiukova ◽  
Elizabeth A. Kulikova ◽  
Alexander V. Kulikov
Keyword(s):  
Locomotor Activity ◽  
Body Mass ◽  
Mrna Level ◽  
Model Organism ◽  
The Body ◽  
Suitable Model ◽  
Mrna Levels ◽  
Age Related ◽  
Nothobranchius Furzeri ◽  
Males And Females

Short-lived turquoise killifish (Nothobranchius furzeri) have become a popular model organism for neuroscience. In the present paper we study for the first time their behavior in the novel tank diving test and the levels of mRNA of various 5-HT-related genes in brains of 2-, 4- and 6-month-old males and females of N. furzeri. The marked effect of age on body mass, locomotor activity and the mRNA level of Tph1b, Tph2, Slc6a4b, Mao, Htr1aa, Htr2a, Htr3a, Htr3b, Htr4, Htr6 genes in the brains of N. furzeri males was shown. Locomotor activity and expression of the Mao gene increased, while expression of Tph1b, Tph2, Slc6a4b, Htr1aa, Htr2a, Htr3a, Htr3b, Htr4, Htr6 genes decreased in 6-month-old killifish. Significant effects of sex on body mass as well as on mRNA level of Tph1a, Tph1b, Tph2, Slc6a4b, Htr1aa, 5-HT2a, Htr3a, Htr3b, Htr4, and Htr6 genes were revealed: in general both the body mass and the expression of these genes were higher in males. N. furzeri is a suitable model with which to study the fundamental problems of age-related alterations in various mRNA levels related with the brains 5-HT system.

Rapid acclimation of the cortisol stress response in adult turquoise killifish Nothobranchius furzeri

2018 ◽  
Vol 53 (4) ◽  
pp. 383-393
Author(s):  
Dallas W Henderson ◽  
Brian C Small
Keyword(s):  
Messenger Rna ◽  
Plasma Cortisol ◽  
Constitutive Expression ◽  
Whole Body ◽  
Stress Axis ◽  
Model Species ◽  
Repeated Stress ◽  
Nothobranchius Furzeri ◽  
The Brain ◽  
Dimorphic Behavior

The turquoise killifish Nothobranchius furzeri is an increasingly popular model species for comparative vertebrate research, and the basic physiology including responses to stressful stimuli are of primary interest. We exposed adult killifish to a single or repeated periods of acute confinement followed by analysis of tissue cortisol and plasma cortisol concentrations. Individuals were also sampled for messenger RNA (mRNA) expression of corticotropin-releasing hormone ( CRH), mineralocorticoid receptor ( MR), and glucocorticoid receptor ( GR) in the brain to examine the effects of repeated stress events on constitutive expression of these important stress axis components. Following a single 30-minute confinement stress, male plasma cortisol significantly differed from baseline ( p = 0.04). Both male and female whole-body cortisol were significantly increased ( p = 0.004 and p = 0.04, respectively) at 15 and 30 minutes poststress. Despite obvious dimorphic behavior and morphology, cortisol concentrations did not differ between the sexes. Exposure to daily repeated confinement for one week altered the cortisol response in both sexes. Time 0, 15, and 60 minutes poststress cortisol concentrations were depressed in repeatedly stressed males ( p ≤ 0.05), and times 0, 30 and 120 minutes poststress cortisol concentrations were depressed in repeatedly stressed females ( p ≤ 0.05). Constitutive expression of CRH, MR, and GR mRNA in the brain following one week of repeated stress events did not differ among treatments or sexes. This study introduces the first description of hypothalamic-pituitary-interrenal axis activity in this important model species. Reduced cortisol production in repeatedly stressed adult killifish suggests acclimation to repeated stressors. Furthermore, acclimation was rapid, and plasma cortisol concentrations altered significantly in as little as one week.

scholarly journals Individual behavioral variation reflects personality divergence in the upcoming model organism Nothobranchius furzeri

2018 ◽  
Vol 8 (16) ◽  
pp. 8448-8457 ◽  
Author(s):  
Eli S. J. Thoré ◽  
Laure Steenaerts ◽  
Charlotte Philippe ◽  
Arnout Grégoir ◽  
Luc Brendonck ◽  
...  
Keyword(s):  
Model Organism ◽  
Behavioral Variation ◽  
Nothobranchius Furzeri

Id(entifying) the inhibitor of DNA binding 3 in the brain of Nothobranchius furzeri upon aging

2020 ◽  
Author(s):  
Adele Leggieri ◽  
Antonio Palladino ◽  
Chiara Attanasio ◽  
Luigi Avallone ◽  
Paolo Girolamo ◽  
...  
Keyword(s):  
Dna Binding ◽  
Nothobranchius Furzeri ◽  
Inhibitor Of Dna Binding ◽  
The Brain

scholarly journals A miRNA catalogue and ncRNA annotation of the short-living fish Nothobranchius furzeri

2017 ◽  
Author(s):  
Mario Baumgart ◽  
Emanuel Barth ◽  
Aurora Savino ◽  
Marco Groth ◽  
Philipp Koch ◽  
...  
Keyword(s):  
Gasterosteus Aculeatus ◽  
Model Organism ◽  
Takifugu Rubripes ◽  
Aging Research ◽  
Control Of Gene Expression ◽  
Non Coding Rna ◽  
Nothobranchius Furzeri ◽  
In Captivity ◽  
Supplementary Material

ABSTRACTBackground: The short-lived fish Nothobranchius furzeri is the shortest-lived vertebrate that can be cultured in captivity and was recently established as a model organism for aging research. Small non-coding RNAs, especially miRNAs, are implicated in age-dependent control of gene expression.Results: Here, we present a comprehensive catalogue of miRNAs and several other non-coding RNA classes (ncRNAs) for Nothobranchius furzeri. Analyzing multiple small RNA-Seq libraries, we show most of these identified miRNAs are expressed in at least one of seven Nothobranchius species. Additionally, duplication and clustering of N. furzeri miRNAs was analyzed and compared to the four fish species Danio rerio, Oryzias latipes, Gasterosteus aculeatus and Takifugu rubripes. A peculiar characteristic of N. furzeri as compared to other teleosts was a duplication of the miR-29 cluster.Conclusion: The completeness of the catalogue we provide is comparable to that of zebrafish. This catalogue represents a basis to investigate the role of miRNAs in aging and development in this species.Availability: All supplementary material can be found online at http://www.rna.uni-jena.de/en/supplements/nothobranchius-furzeri-mirnome/.

scholarly journals Genetics in the Honey Bee: Achievements and Prospects toward the Functional Analysis of Molecular and Neural Mechanisms Underlying Social Behaviors

Insects ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 348 ◽  
Author(s):  
Hiroki Kohno ◽  
Takeo Kubo
Keyword(s):  
Candidate Genes ◽  
Honey Bee ◽  
Genetic Manipulation ◽  
Expression Profiles ◽  
Model Organism ◽  
Social Behaviors ◽  
Forward Genetics ◽  
Mushroom Bodies ◽  
Functional Analyses ◽  
The Brain

The European honey bee is a model organism for studying social behaviors. Comprehensive analyses focusing on the differential expression profiles of genes between the brains of nurse bees and foragers, or in the mushroom bodies—the brain structure related to learning and memory, and multimodal sensory integration—has identified candidate genes related to honey bee behaviors. Despite accumulating knowledge on the expression profiles of genes related to honey bee behaviors, it remains unclear whether these genes actually regulate social behaviors in the honey bee, in part because of the scarcity of genetic manipulation methods available for application to the honey bee. In this review, we describe the genetic methods applied to studies of the honey bee, ranging from classical forward genetics to recently developed gene modification methods using transposon and CRISPR/Cas9. We then discuss future functional analyses using these genetic methods targeting genes identified by the preceding research. Because no particular genes or neurons unique to social insects have been found yet, further exploration of candidate genes/neurons correlated with sociality through comprehensive analyses of mushroom bodies in the aculeate species can provide intriguing targets for functional analyses, as well as insight into the molecular and neural bases underlying social behaviors.

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