Osteogenic Potential of Human Oral-Periosteal Cells (PCs) Isolated From Different Oral Origin: An In Vitro Study

2015 ◽  
Vol 231 (3) ◽  
pp. 607-612 ◽  
Author(s):  
Gabriele Ceccarelli ◽  
Antonio Graziano ◽  
Laura Benedetti ◽  
Marcello Imbriani ◽  
Federica Romano ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Periosteal Cells

Comparing the osteogenic potential of schneiderian membrane and dental pulp mesenchymal stem cells: an in vitro study

2021 ◽  
Author(s):  
Antoine Berbéri ◽  
Joseph Sabbagh ◽  
Rita Bou Assaf ◽  
Michella Ghassibe-Sabbagh ◽  
Fatima Al-Nemer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Schneiderian Membrane

Osteogenic Potential of Non Thermal Biocompatible Atmospheric Pressure Plasma Treated Zirconia: In Vitro Study

2017 ◽  
Vol 7 (8) ◽  
pp. 662-670 ◽  
Author(s):  
Nayansi Jha ◽  
Jin Sung Choi ◽  
Ji Hye Kim ◽  
Ranju Jung ◽  
Eun Ha Choi ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Pressure Plasma

Mesenchymal stem cells with osteogenic potential in human maxillary sinus membrane: an in vitro study

2016 ◽  
Vol 21 (5) ◽  
pp. 1599-1609 ◽  
Author(s):  
Antoine Berbéri ◽  
Fatima Al-Nemer ◽  
Eva Hamade ◽  
Ziad Noujeim ◽  
Bassam Badran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Maxillary Sinus ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Sinus Membrane

scholarly journals Osteogenic Potential of Fast Set Bioceramic Cements: Molecular and In Vitro Study

2020 ◽  
Vol 10 (19) ◽  
pp. 6713
Author(s):  
Damiano Pasqualini ◽  
Allegra Comba ◽  
Laura Annaratone ◽  
Virginia Mola ◽  
Mario Alovisi ◽  
...  
Keyword(s):  
Setting Time ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
Tricalcium Silicate ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
In Vitro Mineralization ◽  
Post Hoc ◽  
The Impact

Recently, pre-mixed bioceramics in fast set formulations have been increasingly utilized in clinical practice as an alternative to mineral trioxide aggregate (MTA) for their shorter setting time and better handling properties. However, the impact on their osteogenic potential, due to modifications in chemical composition to promote a fast setting, is still unclear. This molecular and in vitro study compared the osteogenic potential of root repairing material putty fast set (FSP) with root-repairing material putty (RRMPU), root-repairing material paste (RRMPA), Biodentine™ and MTA. The null hypothesis tested was that there are no differences among the tricalcium silicate materials in terms of osteogenic potential. Standardized discs were cultured with MG-63 human osteoblastic-like cells to assess biocompatibility, the activity of alkaline phosphatase (ALP) and osteogenic potential. Biocompatibility was evaluated at baseline and after 24 and 48 h. Osteogenic differentiation was assessed after 15 days. Data were analyzed with one-way ANOVAs and Tukey’s post-hoc test (p < 0.05). All materials showed biocompatibility and bioactivity. ALP activity, which induces mineral nodule deposition, increased in all the cements tested, with a significant increase in RRMPU (p < 0.001) and FSP (p < 0.001) samples versus MTA. In vitro mineralization was significantly increased for RRMPU (p < 0.0001), FSP (p = 0.00012) and Biodentine™ (p < 0.0001) versus MTA. The bioceramics tested showed higher levels of biocompatibility and bioactivity than MTA; a higher capacity for mineralization was observed with RRMPU and FSP versus MTA.

scholarly journals Evaluation of the osteogenic potential of rat adipose-derived stem cells with different polycaprolactone/alginate-based nanofibrous scaffolds: an in vitro study

2020 ◽  
Vol 7 ◽  
pp. 14-14
Author(s):  
Eman Hany ◽  
Sarah Yahia ◽  
Mahmoud Fathy Elsherbeny ◽  
Nagla Mahmoud Salama ◽  
Islam Mohammed Ateia ◽  
...  
Keyword(s):  
Stem Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Adipose Derived Stem Cells ◽  
Nanofibrous Scaffolds

scholarly journals Human Patellar Bone Dust has Osteogenic Potential: An in vitro Study

2021 ◽  
pp. 246-252
Author(s):  
Mariam Adeel Roghay ◽  
Nuzhat Hassan ◽  
Shumaila Usman ◽  
Syed Nudrat Nawaid Shah ◽  
Kevin Joseph Jerome Borges ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Umbilical Cord ◽  
Rna Extraction ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Human Umbilical Cord ◽  
Differentiated Cells ◽  
Related Transcription Factor

Aims: To substantiate the osteogenic differentiation of human Umbilical cord Mesenchymal Stem Cells (hUMSCs) under the influence of Bone Dust (BD) by detecting expression of genes RUNX2 and IBSP in the differentiated cells. Study Design: In vitro study. Place and Duration of Study: MDRL Cell Culture Lab Ziauddin University and Dr.Zafar H.Zaidi Institute of Proteomics, University of Karachi, between July 2020 and July 2021. Methodology: We designed an innovative experiment to differentiate hUMSCs into osteoblasts under the influence of BD, without using any conventional differentiating medium. The hUMSCs were isolated from umbilical cord and cultured in a 6-well plate with 3μm inserts, in which BD was lodged, preventing direct contact of BD with hUMSCs. The plates with hUMSCs and BD-filled inserts were incubated at 37°C for 14 days after which RNA extraction, cDNA synthesis and qPCR for two genes was performed. Expression of Runt-related transcription factor-2(RUNX2) and Integrin-binding Sialoprotein (IBSP) were quantified. Results: Results revealed an increased expression of the late osteogenic marker IBSP(p=0.01) signifying that the hUMSCs had undergone differentiation into mature osteoblasts. Conclusion: This novel experiment identifies BD as a new source for osteogenic differentiation of hUMSCs in in vitro experiments.

Sign in / Sign up

Export Citation Format

Share Document