Functional measurement of local proteolytic activity in living cells of invasive and non-invasive tumors

2004 ◽  
Vol 202 (3) ◽  
pp. 690-697 ◽  
Author(s):  
Thomas Ludwig ◽  
Sylvia Püttmann ◽  
Helga Bertram ◽  
Lars Tatenhorst ◽  
Werner Paulus ◽  
...  
Keyword(s):  
Proteolytic Activity ◽  
Living Cells ◽  
Functional Measurement ◽  
Non Invasive

A novel microfluidic microelectrode chip for a significantly enhanced monitoring of NPY-receptor activation in live mode

Lab on a Chip ◽  
2017 ◽  
Vol 17 (24) ◽  
pp. 4294-4302 ◽  
Author(s):  
Franziska D. Zitzmann ◽  
Heinz-Georg Jahnke ◽  
Felix Nitschke ◽  
Annette G. Beck-Sickinger ◽  
Bernd Abel ◽  
...  
Keyword(s):  
Real Time ◽  
Microfluidic Chip ◽  
Microelectrode Array ◽  
Fem Simulation ◽  
Receptor Activation ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Non Invasive ◽  
Npy Receptor ◽  
Simulation Based

We present a FEM simulation based step-by-step development of a microelectrode array integrated into a microfluidic chip for the non-invasive real-time monitoring of living cells.

scholarly journals Measurement of Separase Proteolytic Activity in Single Living Cells by a Fluorogenic Flow Cytometry Assay

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0133769 ◽  
Author(s):  
Wiltrud Haaß ◽  
Helga Kleiner ◽  
Martin C. Müller ◽  
Wolf-Karsten Hofmann ◽  
Alice Fabarius ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Proteolytic Activity ◽  
Living Cells ◽  
Flow Cytometry Assay ◽  
Single Living Cells ◽  
Single Living

Direct non-invasive observation of metabolism in living cells by 13C nuclear magnetic resonance spectroscopy

1980 ◽  
Vol 58 (17) ◽  
pp. 1839-1846 ◽  
Author(s):  
Gerardo Burton ◽  
Robert L. Baxter ◽  
J. Martyn Gunn ◽  
Philip J. Sidebottom ◽  
Paul E. Fagerness ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Anaerobic Metabolism ◽  
Aminolevulinic Acid ◽  
Living Cells ◽  
Resonance Spectroscopy ◽  
Whole Cells ◽  
Non Invasive ◽  
13C Nuclear Magnetic Resonance ◽  
C Nmr

Applications of 13C nmr in following the metabolism and fate of 13C enriched substrates in whole cells are described. Studies on the elaboration of coproporphyrinogens I (2a) and III (2b) from [5-13C]-δ-aminolevulinic acid (1) and [11-13C-porphobilinogen (PBG, 3), in Rhodopseudomonasspheroides and Propionibacteriumshermanii, respectively, the randomization of 13C from [3-13C]-propionate by P. shermanii, the biosynthesis of citrate, gentisalcohol (4), and patulin (5) from [2-13C]-acetate by Penicilliumurticae, and the anaerobic metabolism of [1-13C]-glucose in rabbit erythrocytes are reported.

scholarly journals Photoswitchable paclitaxel-based microtubule stabilisers allow optical control over the microtubule cytoskeleton

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Adrian Müller-Deku ◽  
Joyce C. M. Meiring ◽  
Kristina Loy ◽  
Yvonne Kraus ◽  
Constanze Heise ◽  
...  
Keyword(s):  
Intracellular Transport ◽  
Biological Response ◽  
Living Cells ◽  
Optical Control ◽  
Microtubule Cytoskeleton ◽  
Microtubule Network ◽  
Cellular Processes ◽  
Non Invasive ◽  
Chemical Inhibitors ◽  
Neuronal Physiology

Abstract Small molecule inhibitors are prime reagents for studies in microtubule cytoskeleton research, being applicable across a range of biological models and not requiring genetic engineering. However, traditional chemical inhibitors cannot be experimentally applied with spatiotemporal precision suiting the length and time scales inherent to microtubule-dependent cellular processes. We have synthesised photoswitchable paclitaxel-based microtubule stabilisers, whose binding is induced by photoisomerisation to their metastable state. Photoisomerising these reagents in living cells allows optical control over microtubule network integrity and dynamics, cell division and survival, with biological response on the timescale of seconds and spatial precision to the level of individual cells within a population. In primary neurons, they enable regulation of microtubule dynamics resolved to subcellular regions within individual neurites. These azobenzene-based microtubule stabilisers thus enable non-invasive, spatiotemporally precise modulation of the microtubule cytoskeleton in living cells, and promise new possibilities for studying intracellular transport, cell motility, and neuronal physiology.

scholarly journals On the Behaviour of Living Cells under the Influence of Ultrasound

Fluids ◽  
2018 ◽  
Vol 3 (4) ◽  
pp. 82 ◽  
Author(s):  
David Rubin ◽  
Nicole Anderton ◽  
Charl Smalberger ◽  
Jethro Polliack ◽  
Malavika Nathan ◽  
...  
Keyword(s):  
Living Cells ◽  
Review Article ◽  
Medical Ultrasound ◽  
Biological Cells ◽  
Membrane Permeation ◽  
Non Invasive ◽  
Concise Overview ◽  
Ultrasound Technology ◽  
Tissue Structures

Medical ultrasound technology is available, affordable, and non-invasive. It is used to detect, quantify, and heat tissue structures. This review article gives a concise overview of the types of behaviour that biological cells experience under the influence of ultrasound only, i.e., without the presence of microbubbles. The phenomena are discussed from a physics and engineering perspective. They include proliferation, translation, apoptosis, lysis, transient membrane permeation, and oscillation. The ultimate goal of cellular acoustics is the detection, quantification, manipulation and eradication of individual cells.

scholarly journals A Non-Invasive Tool for Real-Time Measurement of Sulfate in Living Cells

2020 ◽  
Vol 21 (7) ◽  
pp. 2572 ◽  
Author(s):  
Urooj Fatima ◽  
Mohammad K. Okla ◽  
Mohd Mohsin ◽  
Ruphi Naz ◽  
Walid Soufan ◽  
...  
Keyword(s):  
Metabolic Network ◽  
Real Time ◽  
Fluorescent Proteins ◽  
Essential Element ◽  
Resonance Energy ◽  
Primary Source ◽  
Essential Amino Acids ◽  
Living Cells ◽  
Live Cells ◽  
Non Invasive

Sulfur (S) is an essential element for all forms of life. It is involved in numerous essential processes because S is considered as the primary source of one of the essential amino acids, methionine, which plays an important role in biological events. For the control and regulation of sulfate in a metabolic network through fluxomics, a non-invasive tool is highly desirable that opens the door to monitor the level of the sulfate in real time and space in living cells without fractionation of the cells or tissue. Here, we engineered a FRET (fluorescence resonance energy transfer) based sensor for sulfate, which is genetically-encoded and named as FLIP-SP (Fluorescent indicator protein for sulfate). The FLIP-SP can measure the level of the sulfate in live cells. This sensor was constructed by the fusion of fluorescent proteins at the N- and C-terminus of sulfate binding protein (sbp). The FLIP-SP is highly specific to sulfate, and showed pH stability. Real-time monitoring of the level of sulfate in prokaryotic and eukaryotic cells showed sensor bio-compatibility with living cells. We expect that this sulfate sensor offers a valuable strategy in the understanding of the regulation of the flux of sulfate in the metabolic network.

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