Non‐invasive analysis of cell cycle dynamics in single living cells with Raman micro‐spectroscopy

2008 ◽  
Vol 104 (4) ◽  
pp. 1427-1438 ◽  
Author(s):  
Robin J. Swain ◽  
Gavin Jell ◽  
Molly M. Stevens
Keyword(s):  
Cell Cycle ◽  
Living Cells ◽  
Non Invasive ◽  
Single Living Cells ◽  
Cell Cycle Dynamics ◽  
Single Living

Synchrotron Fourier transform infrared (FTIR) analysis of single living cells progressing through the cell cycle

The Analyst ◽  
2013 ◽  
Vol 138 (14) ◽  
pp. 3891 ◽  
Author(s):  
Donna R. Whelan ◽  
Keith R. Bambery ◽  
Ljiljana Puskar ◽  
Don McNaughton ◽  
Bayden R. Wood
Keyword(s):  
Cell Cycle ◽  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Living Cells ◽  
Ftir Analysis ◽  
Single Living Cells ◽  
Single Living

The Effect of Enucleation on Flagellar Regeneration in the Protozoon Peranema Trichophorum

1969 ◽  
Vol 4 (1) ◽  
pp. 171-178
Author(s):  
S. L. TAMM
Keyword(s):  
Cell Cycle ◽  
Mitotic Cycle ◽  
Living Cells ◽  
Regeneration Capacity ◽  
Flagellar Regeneration ◽  
Single Living Cells ◽  
Single Living

A rotocompressor was used to enucleate the flagellate protozoon Peranema trichophorum at known stages in the mitotic cycle. This new enucleation technique, combined with recently devised methods for amputating the flagellum and recording its regeneration in single living cells, permitted the investigation of the role of the nucleus in flagellar regeneration at different cell ages. The flagellar regeneration capacity of an enucleate Peranema depended on the stage in the cell cycle when the nucleus was removed. Post-division enucleate cells regenerated about half the length reached by sham-operated controls, and at slower rates, while predivision enucleate cells regenerated flagella equally as well as the controls. Therefore, the nucleus is making an immediate contribution to flagellar regeneration early in the cell cycle, but not late in the cell cycle.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

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