Oncostatin M stimulates proliferation and functions of mouse fetal liver cells in three-dimensional cultures

2004 ◽  
Vol 202 (3) ◽  
pp. 698-706 ◽  
Author(s):  
Tomo Ehashi ◽  
Hirotoshi Miyoshi ◽  
Norio Ohshima
Keyword(s):  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Oncostatin M ◽  
Fetal Liver Cells

scholarly journals Cultivation of Fetal Liver Cells in a Three-Dimensional Poly-L-Lactic Acid Scaffold in the Presence of Oncostatin M

2002 ◽  
Vol 11 (5) ◽  
pp. 403-406 ◽  
Author(s):  
Jinlan Jiang ◽  
Nobuhiko Kojima ◽  
Taisei Kinoshita ◽  
Atsushi Miyajima ◽  
Weiqun Yan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Oncostatin M ◽  
Albumin Secretion ◽  
Plla Scaffold ◽  
Fetal Liver Cells ◽  
Number Of Cells

To investigate the feasibility of fetal liver cells for liver tissue engineering, the supporting function of poly-l-lactic acid (PLLA) for fetal liver cells and the effects of oncostatin M (OSM) on hepatic differentiation were studied. After preparing three-dimensional biodegradable PLLA scaffold having a well-developed open-pore structure by a gas-forming method with ammonium chloride particles as a porogen and a gas-forming reagent, fetal liver cells separated from E14.5-C57BL/6CrSlc murine embryos were inoculated in the PLLA scaffolds. Cells were cultured in Williams' E medium with or without OSM (10 ng/ml) for 30 days with a medium change every 2 days. Results showed that there were significant increases in the number of cells and in albumin secretion in PLLA culture compared with in monolayer culture on day 15. In addition, a significant increase in albumin secretion was observed in OSM-added PLLA culture compared with OSM-free culture, and there was only a slightly enhanced albumin secretion in monolayer cultures with OSM. These results suggest that PLLA may enhance the biological activity of OSM for inducing maturation of fetal liver cells. Interestingly, the number of cells in PLLA culture with OSM decreased compared with OSM-free PLLA culture at day 15. This may be because promotion of hepatic development by OSM simultaneously suppressed in vitro hematopoiesis (i.e., blood cell production). In summary, our results indicate that the three-dimensional PLLA scaffold is a good support material for the cultivation of fetal liver cells and that OSM is capable of not only terminating hematopoiesis of the fetal liver but also stimulating the maturation of hepatic parenchymal cells in vitro.

Expansion of mouse hematopoietic stem/progenitor cells in three-dimensional cocultures on growth-suppressed stromal cell layer

2019 ◽  
Vol 42 (7) ◽  
pp. 374-379 ◽  
Author(s):  
Hirotoshi Miyoshi ◽  
Chiaki Sato ◽  
Yuichiro Shimizu ◽  
Misa Morita
Keyword(s):  
Progenitor Cells ◽  
Mitomycin C ◽  
Stromal Cells ◽  
Fetal Liver ◽  
Three Dimensional ◽  
Hematopoietic Cells ◽  
Liver Cells ◽  
Hematopoietic Progenitor ◽  
Hematopoietic Stem ◽  
Fetal Liver Cells

With the aim of establishing an effective method to expand hematopoietic stem/progenitor cells for application in hematopoietic stem cell transplantation, we performed ex vivo expansion of hematopoietic stem/progenitor cells derived from mouse fetal liver cells in three-dimensional cocultures with stromal cells. In these cocultures, stromal cells were first cultured within three-dimensional scaffolds to form stromal layers and then fetal liver cells containing hematopoietic cells were seeded on these scaffolds to expand the hematopoietic cells over the 2 weeks of coculture in a serum-containing medium without the addition of cytokines. Prior to coculture, stromal cell growth was suppressed by treatment with the DNA synthesis inhibitor mitomycin C, and its effect on hematopoietic stem/progenitor cell expansion was compared with that in control cocultures in which fetal liver cells were cocultured with three-dimensional freeze-thawed stromal cells. After coculture with mitomycin C-treated stromal cells, we achieved a several-fold expansion of the primitive hematopoietic cells (c-kit+hematopoietic progenitor cells >7.8-fold, and CD34+hematopoietic stem/progenitor cells >3.5-fold). Compared with control cocultures, expansion of hematopoietic stem/progenitor cells tended to be lower, although that of hematopoietic progenitor cells was comparable. Thus, our results suggest that three-dimensional freeze-thawed stromal cells have higher potential to expand hematopoietic stem/progenitor cells compared with mitomycin C-treated stromal cells.

Three-Dimensional Perfusion Bioreactor Culture Supports Differentiation of Human Fetal Liver Cells

2010 ◽  
Vol 16 (6) ◽  
pp. 2007-2016 ◽  
Author(s):  
Eva Schmelzer ◽  
Fabio Triolo ◽  
Morris E. Turner ◽  
Robert L. Thompson ◽  
Katrin Zeilinger ◽  
...  
Keyword(s):  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Perfusion Bioreactor ◽  
Bioreactor Culture ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells

Long-term cultures of human fetal liver cells: a three-dimensional experimental model for monitoring liver tissue development

1998 ◽  
Vol 28 (3) ◽  
pp. 480-490 ◽  
Author(s):  
Massimo Sargiacomo ◽  
Paolo Onori ◽  
Elena Bravo ◽  
Antonio Franchitto ◽  
Alfredo Cantafora ◽  
...  
Keyword(s):  
Experimental Model ◽  
Liver Tissue ◽  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Tissue Development ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells

scholarly journals Mouse Fetal Liver Cells in Artificial Capillary Beds in Three-Dimensional Four-Compartment Bioreactors

2005 ◽  
Vol 167 (5) ◽  
pp. 1279-1292 ◽  
Author(s):  
Satdarshan P.S. Monga ◽  
Mariah S. Hout ◽  
Matt J. Baun ◽  
Amanda Micsenyi ◽  
Peggy Muller ◽  
...  
Keyword(s):  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Fetal Liver Cells ◽  
Artificial Capillary

scholarly journals Enhanced In Vitro Maturation of Fetal Mouse Liver Cells with Oncostatin M, Nicotinamide, and Dimethyl Sulfoxide

2002 ◽  
Vol 11 (5) ◽  
pp. 435-441 ◽  
Author(s):  
Y. Sakai ◽  
J. Jiang ◽  
N. Kojima ◽  
T. Kinoshita ◽  
A. Miyajima
Keyword(s):  
In Vitro Maturation ◽  
Fetal Liver ◽  
Liver Cells ◽  
Oncostatin M ◽  
Albumin Secretion ◽  
Ammonium Removal ◽  
Fetal Mouse Liver ◽  
Fetal Liver Cells ◽  
Small Hepatocytes

Although cells isolated from fetal liver are one of the major sources for liver tissue engineering, it is still very difficult to induce them to fully differentiate in vitro into mature hepatocytes. We therefore investigated the effects of nicotinamide (NA), dimethyl sulfoxide (DMSO), and oncostatin M (OSM) on differentiation in terms of the expression of various liver-specific functions, because these factors have been reported to induce the emergence of possible hepatocyte progenitor cells (small hepatocytes) in adult rat hepatocyte culture or maturation of fetal mouse liver cells in culture. Fetal liver cells isolated from mouse embryos were cultured for 5 weeks in collagen-precoated plates. NA (10 mM) and DMSO (1%) remarkably enhanced the emergence of small hepatocytes, and OSM also synergistically enhanced the selective growth of small hepatocytes and inhibited the growth of blood cell populations. In the presence of these three factors, such small hepatocytes became dominant in culture, so that they covered almost 60–70% of confluence after week 2. In addition, some of them piled up over the small hepatocyte monolayer and displayed distinctively differentiated morphology, such as the emergence of binucleated cells, formation of tight gap junctions, and possible bile duct structures. Although OSM alone had very weak effects on hepatocyte functions, albumin secretion and cytochrome P450IA1/2 capacity were greatly enhanced when combined with NA or DMSO. This functional observation closely agreed with the emergence of small hepatocytes. In contrast, ammonium removal was strongly dependent on DMSO alone. DNA amount basis functions of fetal cells with three factors at week 5 were 1/7 for albumin secretion, 3 times higher for ammonium removal, and 1/10 for P450 capacity, compared with those of cultured adult mouse hepatocytes. These results show that inclusion of NA, DMSO, and OSM in the culture medium significantly enhances in vitro maturation of fetal liver cells when compared with conventional culture conditions.

LONG-TERM CULTURE OF FETAL LIVER CELLS USING THREE-DIMENSIONAL POROUS POLYMER

ASAIO Journal ◽  
1999 ◽  
Vol 45 (2) ◽  
pp. 129
Author(s):  
H Miyoshi ◽  
T Ehashi ◽  
H Ema ◽  
H C Hsu ◽  
H Nakauchi ◽  
...  
Keyword(s):  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Porous Polymer ◽  
Long Term Culture ◽  
Fetal Liver Cells
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