Effects of oncostatin M on secretion of vascular endothelial growth factor and reconstruction of liver-like structure by fetal liver cells in monolayer and three-dimensional cultures

2007 ◽  
Vol 82A (1) ◽  
pp. 73-79 ◽  
Author(s):  
Tomo Ehashi ◽  
Toshie Koyama ◽  
Keiko Ookawa ◽  
Norio Ohshima ◽  
Hirotoshi Miyoshi
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Oncostatin M ◽  
Vascular Endothelial ◽  
Fetal Liver Cells ◽  
Endothelial Growth Factor

Early changes in coronary artery wall structure detected by microcomputed tomography in experimental hypercholesterolemia

2007 ◽  
Vol 293 (3) ◽  
pp. H1997-H2003 ◽  
Author(s):  
Xiang-Yang Zhu ◽  
Michael D. Bentley ◽  
Alejandro R. Chade ◽  
Erik L. Ritman ◽  
Amir Lerman ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Coronary Artery ◽  
Growth Factor ◽  
Three Dimensional ◽  
Wall Structure ◽  
Vascular Endothelial ◽  
Micro Ct ◽  
Artery Wall ◽  
Coronary Artery Wall ◽  
Endothelial Growth Factor

Changes in the structure of the artery wall commence shortly after exposure to cardiovascular risk factors, such as hypercholesterolemia (HC), but may be difficult to detect. The ability to study vascular wall structure could be helpful in evaluation of the factors that instigate atherosclerosis and its pathomechanisms. The present study tested the hypothesis that early morphological changes in coronary arteries of hypercholesterolemic (HC) pigs can be detected using the novel X-ray contrast agent OsO4 and three-dimensional micro-computed tomography (CT). Two groups of pigs were studied after they were fed a normal or an HC (2% cholesterol) diet for 12 wk. Hearts were harvested, coronary arteries were injected with 1% OsO4 solution, and cardiac samples (6-μm-thick) were scanned by micro-CT. Layers of the epicardial coronary artery wall, early lesions, and perivascular OsO4 accumulation were determined. Leakage of OsO4 from myocardial microvessels was used to assess vascular permeability, which was correlated with immunoreactivity of vascular endothelial growth factor in corresponding histological cross sections. OsO4 enhanced the visualization of coronary artery wall layers and facilitated detection of early lesions in HC in longitudinal tomographic sections of vascular segments. Increased density of perivascular OsO4 in HC was correlated with increased vascular endothelial growth factor expression and suggested increased microvascular permeability. The use of OsO4 as a contrast agent in micro-CT allows three-dimensional visualization of coronary artery wall structure, early lesion formation, and changes in vascular permeability. Therefore, this technique can be a useful tool in atherosclerosis research.

scholarly journals Oncostatin-M induction of vascular endothelial growth factor expression in astroglioma cells

Oncogene ◽  
2003 ◽  
Vol 22 (50) ◽  
pp. 8117-8124 ◽  
Author(s):  
Pavle Repovic ◽  
Constance Y Fears ◽  
Candece L Gladson ◽  
Etty N Benveniste
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Oncostatin M ◽  
Vascular Endothelial ◽  
Factor Expression ◽  
Growth Factor Expression ◽  
Endothelial Growth Factor ◽  
Astroglioma Cells

A new transacting factor that modulates hypoxia-induced expression of the erythropoietin gene

Blood ◽  
2000 ◽  
Vol 96 (2) ◽  
pp. 491-497 ◽  
Author(s):  
Madhu Gupta ◽  
Paul T. Mungai ◽  
Eugene Goldwasser
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Messenger Rna ◽  
Leucine Zipper ◽  
Fetal Liver ◽  
Specific Interaction ◽  
Vascular Endothelial ◽  
Induced Expression ◽  
Murine Homolog ◽  
Endothelial Growth Factor

Abstract Hypoxia is a strong stimulus for the transcription of a set of genes, including erythropoietin and vascular endothelial growth factor. Here we report on the cloning, functional significance, and expression of a complementary DNA (cDNA) that is involved in hypoxia-mediated expression of these 2 genes. The full-length cDNA encodes a predicted protein of 806 amino acids that contains a leucine zipper motif. This protein, termed HAF for hypoxia-associated factor, binds to a 17-base pair (bp) region of the erythropoietin promoter, which was shown earlier to participate in hypoxia-induced expression of the erythropoietin gene. In Hep3B cells, clones modified to express HAF antisense RNA showed an attenuated response to hypoxia-mediated induction of both erythropoietin and vascular endothelial growth factor transcription. HAF showed sequence-specific interaction with a DNA element in the 5′ untranslated region ofVEGF gene. The HAF 2.6-kilobase (kb) messenger RNA (mRNA) is expressed in most adult tissues. The highest expression occurs in fetal liver and the least in adult liver. HAF is the murine homolog of Sart-1, a 125-kd human protein expressed in the nuclei of normal and malignant cells.

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