Ethylmalonic encephalopathy 1 initiates overactive autophagy in depleted uranium‐induced cytotoxicity in the human embryonic kidney 293 cells

2020 ◽  
Author(s):  
Yuhui Hao ◽  
Jiawei Huang ◽  
Yonghong Ran ◽  
Shuang Wang ◽  
Juan Li ◽  
...  
Keyword(s):  
Depleted Uranium ◽  
Human Embryonic Kidney ◽  
Ethylmalonic Encephalopathy ◽  
293 Cells

scholarly journals Reduced Expression of EXTL2, a Member of the Exostosin (EXT) Family of Glycosyltransferases, in Human Embryonic Kidney 293 Cells Results in Longer Heparan Sulfate Chains

2015 ◽  
Vol 290 (21) ◽  
pp. 13168-13177 ◽  
Author(s):  
Kirankumar Katta ◽  
Tabasum Imran ◽  
Marta Busse-Wicher ◽  
Mona Grønning ◽  
Szymon Czajkowski ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Human Embryonic Kidney ◽  
293 Cells

Regulation of Human Neuronal Calcium Channels by G Protein βγ Subunits Expressed in Human Embryonic Kidney 293 Cells

1997 ◽  
Vol 52 (2) ◽  
pp. 282-291 ◽  
Author(s):  
Lee R. Shekter ◽  
Ronald Taussig ◽  
Samantha E. Gillard ◽  
Richard J. Miller
Keyword(s):  
Calcium Channels ◽  
G Protein ◽  
Human Embryonic Kidney ◽  
293 Cells ◽  
Neuronal Calcium Channels

scholarly journals Bupivacaine inhibits a small conductance calcium‐activated potassium type 2 channel in human embryonic kidney 293 cells

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hongfei Chen ◽  
Zhousheng Jin ◽  
Fangfang Xia ◽  
Zhijian Fu
Keyword(s):  
Free Calcium ◽  
Heart Cells ◽  
Dependent Manner ◽  
Human Embryonic Kidney ◽  
Patch Clamp Technique ◽  
Hek 293 ◽  
293 Cells ◽  
Ic50 Value ◽  
Small Conductance

Abstract Background Bupivacaine blocks many ion channels in the heart muscle, causing severe cardiotoxicity. Small-conductance calcium-activated potassium type 2 channels (SK2 channels) are widely distributed in the heart cells and are involved in relevant physiological functions. However, whether bupivacaine can inhibit SK2 channels is still unclear. This study investigated the effect of bupivacaine on SK2 channels. Methods The SK2 channel gene was transfected into human embryonic kidney 293 cells (HEK-293 cells) with Lipofectamine 2000. The whole-cell patch-clamp technique was used to examine the effect of bupivacaine on SK2 channels. The concentration–response relationship of bupivacaine for inhibiting SK2 currents (0 mV) was fitted to a Hill equation, and the half-maximal inhibitory concentration (IC50) value was determined. Results Bupivacaine inhibited the SK2 channels reversibly in a dose-dependent manner. The IC50 value of bupivacaine, ropivacaine, and lidocaine on SK2 currents was 16.5, 46.5, and 77.8µM, respectively. The degree of SK2 current inhibition by bupivacaine depended on the intracellular concentration of free calcium. Conclusions The results of this study suggested the inhibitory effect of bupivacaine on SK2 channels. Future studies should explore the effects of SK2 on bupivacaine cardiotoxicity.

Endothelin-B receptors activate Gα13

1999 ◽  
Vol 276 (4) ◽  
pp. C930-C937 ◽  
Author(s):  
Kenichiro Kitamura ◽  
Naoki Shiraishi ◽  
William D. Singer ◽  
Mary E. Handlogten ◽  
Kimio Tomita ◽  
...  
Keyword(s):  
G Protein ◽  
G Proteins ◽  
Guanine Nucleotides ◽  
Heterotrimeric G Proteins ◽  
Human Embryonic Kidney ◽  
Cellular Effects ◽  
293 Cells ◽  
Specific Antagonist ◽  
Α Chain ◽  
Endothelin B

Endothelin (ET) receptors activate heterotrimeric G proteins that are members of the Gi, Gq, and Gs families but may also activate members of other families such as Gα12/13. Gα13 has multiple complex cellular effects that are similar to those of ET. We studied the ability of ET receptors to activate Gα13 using an assay for G protein α-chain activation that is based on the fact that an activated (GTP-bound) α-chain is resistant to trypsinization compared with an inactive (GDP-bound) α-chain. Nonhydrolyzable guanine nucleotides and AlMgF protected Gα13 from degradation by trypsin. In membranes from human embryonic kidney 293 cells that coexpress ETB receptors and α13, ET-3 and 5′-guanylylimidodiphosphate [Gpp(NH)p] increased the protection of α13 compared with Gpp(NH)p alone. The specificity of ETBreceptor-α13 coupling was documented by showing that β2receptors and isoproterenol or ETAreceptors and ET-1 did not activate α13 and that a specific antagonist for ETB receptors blocked ET-3-dependent activation of α13.

Opioid Regulation of the Mouse δ-Opioid Receptor Expressed in Human Embryonic Kidney 293 Cells

1997 ◽  
Vol 52 (2) ◽  
pp. 272-281 ◽  
Author(s):  
George Bot ◽  
Allan D. Blake ◽  
Shuixing Li ◽  
Terry Reisine
Keyword(s):  
Opioid Receptor ◽  
Human Embryonic Kidney ◽  
293 Cells ◽  
Δ Opioid Receptor

Arginine-Glycine-Aspartic Acid Mimics Can Identify a Transitional Activation State of Recombinant αIIbβ3 in Human Embryonic Kidney 293 Cells

1997 ◽  
Vol 52 (2) ◽  
pp. 227-236 ◽  
Author(s):  
Dicky G. Abraham ◽  
Elka M. Nutt ◽  
Rodney A. Bednar ◽  
Bohumil Bednar ◽  
Robert J. Gould ◽  
...  
Keyword(s):  
Aspartic Acid ◽  
Human Embryonic Kidney ◽  
293 Cells ◽  
Arginine Glycine Aspartic Acid

Regulation of BKcaChannels Expressed in Human Embryonic Kidney 293 Cells by Epoxyeicosatrienoic Acid

2001 ◽  
Vol 59 (1) ◽  
pp. 16-23 ◽  
Author(s):  
Mitsuhiro Fukao ◽  
Helen S. Mason ◽  
James L. Kenyon ◽  
Burton Horowitz ◽  
Kathleen D. Keef
Keyword(s):  
Epoxyeicosatrienoic Acid ◽  
Human Embryonic Kidney ◽  
293 Cells
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