Arginine-Glycine-Aspartic Acid Mimics Can Identify a Transitional Activation State of Recombinant αIIbβ3 in Human Embryonic Kidney 293 Cells

1997 ◽  
Vol 52 (2) ◽  
pp. 227-236 ◽  
Author(s):  
Dicky G. Abraham ◽  
Elka M. Nutt ◽  
Rodney A. Bednar ◽  
Bohumil Bednar ◽  
Robert J. Gould ◽  
...  
Keyword(s):  
Aspartic Acid ◽  
Human Embryonic Kidney ◽  
293 Cells ◽  
Arginine Glycine Aspartic Acid

scholarly journals Reduced Expression of EXTL2, a Member of the Exostosin (EXT) Family of Glycosyltransferases, in Human Embryonic Kidney 293 Cells Results in Longer Heparan Sulfate Chains

2015 ◽  
Vol 290 (21) ◽  
pp. 13168-13177 ◽  
Author(s):  
Kirankumar Katta ◽  
Tabasum Imran ◽  
Marta Busse-Wicher ◽  
Mona Grønning ◽  
Szymon Czajkowski ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Human Embryonic Kidney ◽  
293 Cells

scholarly journals Constraining the conformation of peptides with Au nanorods to construct multifunctional therapeutic agents with targeting, imaging, and photothermal abilities

RSC Advances ◽  
2018 ◽  
Vol 8 (47) ◽  
pp. 26517-26522
Author(s):  
Linlin Xie ◽  
Xiaomin Zhi ◽  
Nao Xiao ◽  
Chen-Jie Fang ◽  
Chun-Hua Yan
Keyword(s):  
Aspartic Acid ◽  
Gold Nanorods ◽  
Therapeutic Agents ◽  
Au Nanorods ◽  
Arginine Glycine Aspartic Acid

We demonstrated an easy-to-use strategy to constrain the freedom of an RGD (arginine, glycine, aspartic acid) sequence with gold nanorods.

Regulation of Human Neuronal Calcium Channels by G Protein βγ Subunits Expressed in Human Embryonic Kidney 293 Cells

1997 ◽  
Vol 52 (2) ◽  
pp. 282-291 ◽  
Author(s):  
Lee R. Shekter ◽  
Ronald Taussig ◽  
Samantha E. Gillard ◽  
Richard J. Miller
Keyword(s):  
Calcium Channels ◽  
G Protein ◽  
Human Embryonic Kidney ◽  
293 Cells ◽  
Neuronal Calcium Channels

scholarly journals Bupivacaine inhibits a small conductance calcium‐activated potassium type 2 channel in human embryonic kidney 293 cells

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hongfei Chen ◽  
Zhousheng Jin ◽  
Fangfang Xia ◽  
Zhijian Fu
Keyword(s):  
Free Calcium ◽  
Heart Cells ◽  
Dependent Manner ◽  
Human Embryonic Kidney ◽  
Patch Clamp Technique ◽  
Hek 293 ◽  
293 Cells ◽  
Ic50 Value ◽  
Small Conductance

Abstract Background Bupivacaine blocks many ion channels in the heart muscle, causing severe cardiotoxicity. Small-conductance calcium-activated potassium type 2 channels (SK2 channels) are widely distributed in the heart cells and are involved in relevant physiological functions. However, whether bupivacaine can inhibit SK2 channels is still unclear. This study investigated the effect of bupivacaine on SK2 channels. Methods The SK2 channel gene was transfected into human embryonic kidney 293 cells (HEK-293 cells) with Lipofectamine 2000. The whole-cell patch-clamp technique was used to examine the effect of bupivacaine on SK2 channels. The concentration–response relationship of bupivacaine for inhibiting SK2 currents (0 mV) was fitted to a Hill equation, and the half-maximal inhibitory concentration (IC50) value was determined. Results Bupivacaine inhibited the SK2 channels reversibly in a dose-dependent manner. The IC50 value of bupivacaine, ropivacaine, and lidocaine on SK2 currents was 16.5, 46.5, and 77.8µM, respectively. The degree of SK2 current inhibition by bupivacaine depended on the intracellular concentration of free calcium. Conclusions The results of this study suggested the inhibitory effect of bupivacaine on SK2 channels. Future studies should explore the effects of SK2 on bupivacaine cardiotoxicity.

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