Opioid Regulation of the Mouse δ-Opioid Receptor Expressed in Human Embryonic Kidney 293 Cells

1997 ◽  
Vol 52 (2) ◽  
pp. 272-281 ◽  
Author(s):  
George Bot ◽  
Allan D. Blake ◽  
Shuixing Li ◽  
Terry Reisine
Keyword(s):  
Opioid Receptor ◽  
Human Embryonic Kidney ◽  
293 Cells ◽  
Δ Opioid Receptor

scholarly journals Proteasome Involvement in Agonist-induced Down-regulation of μ and δ Opioid Receptors

2001 ◽  
Vol 276 (15) ◽  
pp. 12345-12355 ◽  
Author(s):  
Kirti Chaturvedi ◽  
Persis Bandari ◽  
Norihiro Chinen ◽  
Richard D. Howells
Keyword(s):  
Opioid Receptor ◽  
Opioid Receptors ◽  
Proteasome Inhibitors ◽  
Metabolic Labeling ◽  
Down Regulation ◽  
Hek 293 ◽  
293 Cells ◽  
Ubiquitin Proteasome ◽  
Μ Opioid Receptor ◽  
Δ Opioid Receptor

This study investigated the mechanism of agonist-induced opioid receptor down-regulation. Incubation of HEK 293 cells expressing FLAG-tagged δ and μ receptors with agonists caused a time-dependent decrease in opioid receptor levels assayed by immunoblotting. Pulse-chase experiments using [35S]methionine metabolic labeling indicated that the turnover rate of δ receptors was accelerated 5-fold following agonist stimulation. Inactivation of functional Giand Goproteins by pertussis toxin-attenuated down-regulation of the μ opioid receptor, while down-regulation of the δ opioid receptor was unaffected. Pretreatment of cells with inhibitors of lysosomal proteases, calpain, and caspases had little effect on μ and δ opioid receptor down-regulation. In marked contrast, pretreatment with proteasome inhibitors attenuated agonist-induced μ and δ receptor down-regulation. In addition, incubation of cells with proteasome inhibitors in the absence of agonists increased steady-state μ and δ opioid receptor levels. Immunoprecipitation of μ and δ opioid receptors followed by immunoblotting with ubiquitin antibodies suggested that preincubation with proteasome inhibitors promoted accumulation of polyubiquitinated receptors. These data provide evidence that the ubiquitin/proteasome pathway plays a role in agonist-induced down-regulation and basal turnover of opioid receptors.

scholarly journals Differential Pharmacological Actions of Methadone and Buprenorphine in Human Embryonic Kidney 293 Cells Coexpressing Human μ-Opioid and Opioid Receptor-Like 1 Receptors

2011 ◽  
Vol 36 (11) ◽  
pp. 2008-2021 ◽  
Author(s):  
Cynthia Wei-Sheng Lee ◽  
Jia-Ying Yan ◽  
Yao-Chang Chiang ◽  
Tsai-Wei Hung ◽  
Hung-Li Wang ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Human Embryonic Kidney ◽  
293 Cells

Agonist-Selective Mechanisms of μ-Opioid Receptor Desensitization in Human Embryonic Kidney 293 Cells

2006 ◽  
Vol 70 (2) ◽  
pp. 676-685 ◽  
Author(s):  
Elizabeth A. Johnson ◽  
Sue Oldfield ◽  
Ellen Braksator ◽  
Ana Gonzalez-Cuello ◽  
Daniel Couch ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Receptor Desensitization ◽  
Human Embryonic Kidney ◽  
293 Cells ◽  
Μ Opioid Receptor

ENKEPHALIN-INDUCED DESENSITIZATION OF ADENYLATE CYCLASE IN RECOMBINANT CV CELLS EXPRESSING A MOUSE δ-OPIOID RECEPTOR

Analgesia ◽  
1995 ◽  
Vol 1 (4) ◽  
pp. 603-606
Author(s):  
Vincent Nappey ◽  
Jean-luc Butour ◽  
Christiane Moisand ◽  
Claire Gaveriaux-Ruff ◽  
Brigitte Kieffer ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Opioid Receptor ◽  
Δ Opioid Receptor
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