Bioengineered corneal epithelial cell sheet from mesenchymal stem cells—A functional alternative to limbal stem cells for ocular surface reconstruction

2019 ◽  
Vol 108 (3) ◽  
pp. 1033-1045 ◽  
Author(s):  
Balu Venugopal ◽  
Sachin J. Shenoy ◽  
Sumitha Mohan ◽  
P. R. Anil Kumar ◽  
T. V. Kumary
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Epithelial Cell ◽  
Surface Reconstruction ◽  
Ocular Surface ◽  
Cell Sheet ◽  
Corneal Epithelial Cell ◽  
Limbal Stem Cells ◽  
Corneal Epithelial ◽  
Ocular Surface Reconstruction

Human Anterior Lens Capsule as a Biologic Substrate for the Ex Vivo Expansion of Limbal Stem Cells in Ocular Surface Reconstruction

Cornea ◽  
2007 ◽  
Vol 26 (4) ◽  
pp. 473-478 ◽  
Author(s):  
Ahmed Galal ◽  
Juan J Perez-Santonja ◽  
Jose Luis Rodriguez-Prats ◽  
Marta Abad ◽  
Jorge Alio
Keyword(s):  
Stem Cells ◽  
Surface Reconstruction ◽  
Ocular Surface ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Lens Capsule ◽  
Limbal Stem Cells ◽  
Ocular Surface Reconstruction ◽  
Anterior Lens Capsule

scholarly journals Therapeutic effect of secretome from TNF-α stimulated mesenchymal stem cells in an experimental model of corneal limbal stem cell deficiency

2021 ◽  
Vol 14 (2) ◽  
pp. 179-185
Author(s):  
Guo-Hu Di ◽  
◽  
Jing Xu ◽  
Chao-Qun Yu ◽  
Qi-Long Cao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Epithelial Cell ◽  
Inflammatory Cell ◽  
Corneal Epithelial Cell ◽  
Corneal Epithelial ◽  
Tnf Α ◽  
Epithelial Cell Apoptosis ◽  
Limbal Stem Cell

AIM: To explore the secretome efficacy in tumor necrosis factor (TNF)-α stimulated mouse mesenchymal stem cells (MSCs) in a murine model of corneal limbal alkali injury. METHODS: Corneal limbal stem cell deficiency (LSCD) was created in the eyes of male C57 mice. Concentrated conditioned medium from TNF-α stimulated MSCs (MSC-CMT) was applied topically for 4wk, with basal medium and conditioned medium from MSCs as controls. Corneal opacification, corneal inflammatory response, and corneal neovascularization (NV) were evaluated. Corneal epithelial cell apoptosis, corneal conjunctivation, and inflammatory cell infiltration were assessed with TUNEL staining, CK3 and Muc-5AC immunostaining, and CD11b immunofluorescence staining, respectively. The effect of TSG-6 was further evaluated by knockdown with short hairpin RNA (shRNA). RESULTS: Compared to the controls, topical administration of MSC-CMT significantly ameliorated the clinical symptoms of alkali-induced LSCD, with restrained corneal NV, reduced corneal epithelial cell apoptosis, and inhibition of corneal conjunctivation. In addition, MSC-CMT treatment significantly reduced CD11b+ inflammatory cell infiltration, and inhibited the expression of pro-inflammatory cytokines (IL-1β, TNF-α and IL-6). Furthermore, the promotion of corneal epithelial reconstruction by MSC-CMT was largely abolished by TSG-6 knockdown. CONCLUSION: Our study provides evidence that MSC-CMT enhances the alleviation of corneal alkali injuries, partially through TSG-6-mediated anti-inflammatory protective mechanisms. MSC-CMT may serve as a potential strategy for treating corneal disorders.

scholarly journals Efficacy of cultivated corneal epithelial stem cells for ocular surface reconstruction

2012 ◽  
pp. 1483 ◽  
Author(s):  
Suksri Chotikavanich ◽  
Prabhasawat ◽  
Pattama Ekpo ◽  
Uiprasertkul ◽  
Nattaporn Tesavibul
Keyword(s):  
Stem Cells ◽  
Surface Reconstruction ◽  
Ocular Surface ◽  
Epithelial Stem Cells ◽  
Corneal Epithelial ◽  
Ocular Surface Reconstruction

scholarly journals Ocular surface reconstruction with suspended limbal stem cells delivered by sutureless fixated amniotic membrane patch

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S79-S79
Author(s):  
Bingqian Liu ◽  
Jian Ge ◽  
Zhichong Wang ◽  
Xuerong Sun ◽  
Ruzhang Jiang
Keyword(s):  
Stem Cells ◽  
Surface Reconstruction ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Limbal Stem Cells ◽  
Ocular Surface Reconstruction ◽  
Membrane Patch
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