Comparison of the canine corneal epithelial cell sheets cultivated from limbal stem cells on canine amniotic membrane, atelocollagen gel, and temperature-responsive culture dish

2014 ◽  
Vol 18 (4) ◽  
pp. 317-325 ◽  
Author(s):  
Eunryel Nam ◽  
Naoki Fujita ◽  
Maresuke Morita ◽  
Keiko Tsuzuki ◽  
Hsing Yi Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cell ◽  
Amniotic Membrane ◽  
Corneal Epithelial Cell ◽  
Culture Dish ◽  
Cell Sheets ◽  
Limbal Stem Cells ◽  
Corneal Epithelial ◽  
Temperature Responsive

Cultivation of corneal epithelial cell sheets on canine amniotic membrane

2012 ◽  
Vol 16 (4) ◽  
pp. 263-268 ◽  
Author(s):  
Eunryel Nam ◽  
Ayaka Takahashi ◽  
Naoki Fujita ◽  
Keiko Tsuzuki ◽  
Ryohei Nishimura
Keyword(s):  
Epithelial Cell ◽  
Amniotic Membrane ◽  
Corneal Epithelial Cell ◽  
Cell Sheets ◽  
Corneal Epithelial

Development of transplantable genetically modified corneal epithelial cell sheets for gene therapy

Biomaterials ◽  
2007 ◽  
Vol 28 (4) ◽  
pp. 745-749 ◽  
Author(s):  
Katsuhiko Watanabe ◽  
Masayuki Yamato ◽  
Yasutaka Hayashida ◽  
Joseph Yang ◽  
Akihiko Kikuchi ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Epithelial Cell ◽  
Genetically Modified ◽  
Corneal Epithelial Cell ◽  
Cell Sheets ◽  
Corneal Epithelial

scholarly journals Therapeutic effect of secretome from TNF-α stimulated mesenchymal stem cells in an experimental model of corneal limbal stem cell deficiency

2021 ◽  
Vol 14 (2) ◽  
pp. 179-185
Author(s):  
Guo-Hu Di ◽  
◽  
Jing Xu ◽  
Chao-Qun Yu ◽  
Qi-Long Cao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Epithelial Cell ◽  
Inflammatory Cell ◽  
Corneal Epithelial Cell ◽  
Corneal Epithelial ◽  
Tnf Α ◽  
Epithelial Cell Apoptosis ◽  
Limbal Stem Cell

AIM: To explore the secretome efficacy in tumor necrosis factor (TNF)-α stimulated mouse mesenchymal stem cells (MSCs) in a murine model of corneal limbal alkali injury. METHODS: Corneal limbal stem cell deficiency (LSCD) was created in the eyes of male C57 mice. Concentrated conditioned medium from TNF-α stimulated MSCs (MSC-CMT) was applied topically for 4wk, with basal medium and conditioned medium from MSCs as controls. Corneal opacification, corneal inflammatory response, and corneal neovascularization (NV) were evaluated. Corneal epithelial cell apoptosis, corneal conjunctivation, and inflammatory cell infiltration were assessed with TUNEL staining, CK3 and Muc-5AC immunostaining, and CD11b immunofluorescence staining, respectively. The effect of TSG-6 was further evaluated by knockdown with short hairpin RNA (shRNA). RESULTS: Compared to the controls, topical administration of MSC-CMT significantly ameliorated the clinical symptoms of alkali-induced LSCD, with restrained corneal NV, reduced corneal epithelial cell apoptosis, and inhibition of corneal conjunctivation. In addition, MSC-CMT treatment significantly reduced CD11b+ inflammatory cell infiltration, and inhibited the expression of pro-inflammatory cytokines (IL-1β, TNF-α and IL-6). Furthermore, the promotion of corneal epithelial reconstruction by MSC-CMT was largely abolished by TSG-6 knockdown. CONCLUSION: Our study provides evidence that MSC-CMT enhances the alleviation of corneal alkali injuries, partially through TSG-6-mediated anti-inflammatory protective mechanisms. MSC-CMT may serve as a potential strategy for treating corneal disorders.

scholarly journals Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells

2017 ◽  
Vol 12 (4) ◽  
pp. 683-696 ◽  
Author(s):  
Ryuhei Hayashi ◽  
Yuki Ishikawa ◽  
Ryousuke Katori ◽  
Yuzuru Sasamoto ◽  
Yuki Taniwaki ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Ips Cells ◽  
Corneal Epithelial Cell ◽  
Cell Sheets ◽  
Corneal Epithelial ◽  
Cell Lineages ◽  
Human Ips Cells

Effects of Cryopreservation on Histology and Viability of Cultured Corneal Epithelial Cell Sheets in Rabbit

Cornea ◽  
2005 ◽  
Vol 24 (6) ◽  
pp. 735-741 ◽  
Author(s):  
Kazuhiro Kito ◽  
Hideaki Kagami ◽  
Chiaki Kobayashi ◽  
Minoru Ueda ◽  
Hiroko Terasaki
Keyword(s):  
Epithelial Cell ◽  
Corneal Epithelial Cell ◽  
Cell Sheets ◽  
Corneal Epithelial
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