In vitro evaluation of the biological compatibility and antibacterial activity of a bone substitute material consisting of silver-doped hydroxyapatite and Bio-Oss®

2017 ◽  
Vol 106 (1) ◽  
pp. 410-420 ◽  
Author(s):  
Jingjue Gong ◽  
Lei Yang ◽  
Qi He ◽  
Ting Jiao
Keyword(s):  
Antibacterial Activity ◽  
Bone Substitute ◽  
Bone Substitute Material ◽  
In Vitro Evaluation ◽  
Biological Compatibility ◽  
Substitute Material

scholarly journals In vivo and in vitro evaluation of flexible, cottonwool-like nanocomposites as bone substitute material for complex defects

2009 ◽  
Vol 5 (5) ◽  
pp. 1775-1784 ◽  
Author(s):  
Oliver D. Schneider ◽  
Franz Weber ◽  
Tobias J. Brunner ◽  
Stefan Loher ◽  
Martin Ehrbar ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Bone Substitute Material ◽  
In Vitro Evaluation ◽  
Substitute Material

Gelatine modified monetite as a bone substitute material: An in vitro assessment of bone biocompatibility

2016 ◽  
Vol 32 ◽  
pp. 275-285 ◽  
Author(s):  
Benjamin Kruppke ◽  
Jana Farack ◽  
Alena-Svenja Wagner ◽  
Sarah Beckmann ◽  
Christiane Heinemann ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Bone Substitute Material ◽  
In Vitro Assessment ◽  
Substitute Material

scholarly journals Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

2020 ◽  
Vol 35 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Solomiya Kyyak ◽  
Sebastian Blatt ◽  
Andreas Pabst ◽  
Daniel Thiem ◽  
Bilal Al-Nawas ◽  
...  
Keyword(s):  
Bone Substitute ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Substitute Material ◽  
Platelet Rich Fibrin ◽  
Osteoblast Activity ◽  
Substitute Material ◽  
Xenogenic Bone

The aim of the in vitro study was a comparison of an allogenic (ABSM) and a xenogenic bone substitute material (XBSM) with and without injectable platelet-rich fibrin (ABSM-i-PRF & XBSM-i-PRF) on cell characteristics of human osteoblasts (HOB). Here, ABSM and XBSM (+ i-PRF = test; - i-PRF = control) were incubated with HOB for 3, 7 and 10 days. HOB viability, migration, proliferation and differentiation (RT-PCR on alkaline phosphatase (AP), bone morphogenetic protein 2 (BMP-2) and osteonectin (OCN)) were measured and compared between groups. At day 3, an increased viability, migration and proliferation was seen for ABSM-i-PRF. For viability and proliferation (days 7 and 10) and for migration (day 10), ABSM-i-PRF/XBSM-i-PRF showed higher values compared to ABSM/XBSM with maximum values for ABSM-i-PRF and minimum values for XBSM. At days 3 and 7, the highest expression of AP was detected in ABSM-i-PRF/XBSM-i-PRF when compared to ABSM/XBSM, whereas at day 10, AP expression levels were elevated in ABSM-i-PRF/ABSM. The highest BMP-2 expression was seen in ABSM-i-PRF whereas OCN expression showed higher levels in ABSM-i-PRF/XBSM-i-PRF at days 3 and 7 with lowest expression for ABSM. Later on, elevated OC levels were detected for ABSM-i-PRF only. In conclusion, i-PRF in combination with ABSM enhances HOB activity when compared to XBSM-i-PRF or untreated BSM in vitro. Therefore, addition of i-PRF to ABSM and – to a lower extent – to XBSM may influence osteoblast activity in vivo.

Combined study on the action and mechanism of G-Rg1/Sr-CaS bone substitute material for ossification and pro-vascularization

2020 ◽  
Vol 10 (2) ◽  
pp. 177-189
Author(s):  
Xue Li ◽  
Shi Hong ◽  
Caixia Tan ◽  
Bo Peng ◽  
Zhengjie Wu ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Research Work ◽  
Calcium Sulphate ◽  
Control Group ◽  
Mouse Bone Marrow ◽  
Bone Substitute Material ◽  
Substitute Material

Neovascularization is important for bone repair, vascularization, and ossification during bone repair. Ginsenoside Rg1 (G-Rg1), which is the main extract of ginseng, has been shown to promote therapeutic angiogenesis. It has been studied in the field of biomaterials, but there is no relevant report in the field of bone substitute materials. In this study, we successfully prepared the bone substitute material combining calcium sulphate (Sr-CaS) with G-Rg1 on the basis of previous research work. In vitro experiments were carried out to verify the ossification of composites by using mouse bone marrow mesenchymal stem cells (BMMSCs) and the ossification was quantified by western blot. The related proteins in the key signaling pathways for the different concentrations of G-Rg1/Sr-CaS composite extract were studied to determine whether there was receptor competition and to find the optimal ratio parameters. The vascularization of the composite was verified in the human umbilical vein endothelial cells (HUVECs) model, and finally the coordination of pro-vascularization and ossification was evaluated in the mouse critical bone defect model. The results indicated that G-Rg1/Sr-CaS composites contributed to ossification in the mouse BMMSC model and vascularization in the HUVEC model. The G-Rg1/Sr-CaS composites resulted in significantly greater bone mineral densities and bone volume/total volume of the defect group compared to the control group. Histological analysis showed that the G-Rg1/SrCaS was resorbable with satisfactory biocompatibility. The doped strontium ions enhanced the bone repair performance of G-Rg1/Sr-CaS in the mouse model and the new substitute demonstrated promising results for clinical use.

scholarly journals In Vitro and In Vivo Evaluation of Starfish Bone-Derived β-Tricalcium Phosphate as a Bone Substitute Material

Materials ◽  
2019 ◽  
Vol 12 (11) ◽  
pp. 1881 ◽  
Author(s):  
Haruka Ishida ◽  
Hisao Haniu ◽  
Akari Takeuchi ◽  
Katsuya Ueda ◽  
Mahoko Sano ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Bone Substitute ◽  
Fibrous Tissue ◽  
Bone Substitute Material ◽  
In Vivo Evaluation ◽  
Tissue Invasion ◽  
Substitute Material ◽  
Porous Calcium Carbonate

We evaluated starfish-derived β-tricalcium phosphate (Sf-TCP) obtained by phosphatization of starfish-bone-derived porous calcium carbonate as a potential bone substitute material. The Sf-TCP had a communicating pore structure with a pore size of approximately 10 μm. Although the porosity of Sf-TCP was similar to that of Cerasorb M (CM)—a commercially available β-TCP bone filler—the specific surface area was roughly three times larger than that of CM. Observation by scanning electron microscopy showed that pores communicated to the inside of the Sf-TCP. Cell growth tests showed that Sf-TCP improved cell proliferation compared with CM. Cells grown on Sf-TCP showed stretched filopodia and adhered; cells migrated both to the surface and into pores. In vivo, vigorous tissue invasion into pores was observed in Sf-TCP, and more fibrous tissue was observed for Sf-TCP than CM. Moreover, capillary formation into pores was observed for Sf-TCP. Thus, Sf-TCP showed excellent biocompatibility in vitro and more vigorous bone formation in vivo, indicating the possible applications of this material as a bone substitute. In addition, our findings suggested that mimicking the microstructure derived from whole organisms may facilitate the development of superior artificial bone.

Melt-Derived Condensed Polymorphic Calcium Phosphate as Bone Substitute Material: An In Vitro Study

2011 ◽  
Vol 94 (9) ◽  
pp. 3023-3029 ◽  
Author(s):  
Anbalagan Balamurugan ◽  
Khalil El Mabrouk ◽  
Sandra Pina ◽  
Mostapha M. Bousmina ◽  
Jose M. F. Ferreira
Keyword(s):  
Calcium Phosphate ◽  
Bone Substitute ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bone Substitute Material ◽  
Substitute Material

In Vitro Evaluation of the Antibacterial Activity and Antibiotic- Modulatory Effect of Gracilaria cervicornis (Tuner) J. Agardh

2018 ◽  
Vol 15 (3) ◽  
Author(s):  
Israeliane F. Cordeiro ◽  
Henrique D. M. Coutinho ◽  
Joao V. A. Ferreira ◽  
Lucindo J. Quintans Junior ◽  
Irwin R. A. Menezes ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
In Vitro Evaluation
Sign in / Sign up

Export Citation Format

Share Document