Combined study on the action and mechanism of G-Rg1/Sr-CaS bone substitute material for ossification and pro-vascularization

2020 ◽  
Vol 10 (2) ◽  
pp. 177-189
Author(s):  
Xue Li ◽  
Shi Hong ◽  
Caixia Tan ◽  
Bo Peng ◽  
Zhengjie Wu ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Research Work ◽  
Calcium Sulphate ◽  
Control Group ◽  
Mouse Bone Marrow ◽  
Bone Substitute Material ◽  
Substitute Material

Neovascularization is important for bone repair, vascularization, and ossification during bone repair. Ginsenoside Rg1 (G-Rg1), which is the main extract of ginseng, has been shown to promote therapeutic angiogenesis. It has been studied in the field of biomaterials, but there is no relevant report in the field of bone substitute materials. In this study, we successfully prepared the bone substitute material combining calcium sulphate (Sr-CaS) with G-Rg1 on the basis of previous research work. In vitro experiments were carried out to verify the ossification of composites by using mouse bone marrow mesenchymal stem cells (BMMSCs) and the ossification was quantified by western blot. The related proteins in the key signaling pathways for the different concentrations of G-Rg1/Sr-CaS composite extract were studied to determine whether there was receptor competition and to find the optimal ratio parameters. The vascularization of the composite was verified in the human umbilical vein endothelial cells (HUVECs) model, and finally the coordination of pro-vascularization and ossification was evaluated in the mouse critical bone defect model. The results indicated that G-Rg1/Sr-CaS composites contributed to ossification in the mouse BMMSC model and vascularization in the HUVEC model. The G-Rg1/Sr-CaS composites resulted in significantly greater bone mineral densities and bone volume/total volume of the defect group compared to the control group. Histological analysis showed that the G-Rg1/SrCaS was resorbable with satisfactory biocompatibility. The doped strontium ions enhanced the bone repair performance of G-Rg1/Sr-CaS in the mouse model and the new substitute demonstrated promising results for clinical use.

scholarly journals Bone Augmentation of Peri-Implant Dehiscence Defects Using Multilaminated Small Intestinal Submucosa as a Barrier Membrane: An Experimental Study in Dogs

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Siwen Wang ◽  
Weiyi Wu ◽  
Yuhua Liu ◽  
Xinzhi Wang ◽  
Lin Tang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Substitute ◽  
Small Intestinal Submucosa ◽  
Control Group ◽  
Collagen Membrane ◽  
Micro Ct ◽  
Bone Substitute Material ◽  
Substitute Material ◽  
Small Intestinal ◽  
Intestinal Submucosa

Objective. The aim of the study is to evaluate the effects of multilaminated small intestinal submucosa (mSIS) combined with bone substitute material to repair peri-implant defects during guided bone regeneration procedures. Methods. Twelve implants were placed in bilateral lower premolars of three beagle dogs, and a peri-implant buccal bone defect (3 mm width and 4 mm height) was created at each implant site. A total of 12 sites were filled with a particulate bone substitute material and then randomly divided into three treatment groups: covered by mSIS membrane (mSIS group), covered by collagen membrane (BG group), and no treatment (control group), each group of four sites. After 12 weeks of healing, all of the animals were euthanized and dissected blocks were obtained for micro-computed tomography (micro-CT) and histological analyses. Results. Micro-CT results revealed similar horizontal width of augmented tissue and new bone formation between mSIS and BG groups (P<0.05). Histological analyses revealed that the differences in horizontal widths of newly formed bone and bone-to-implant contact between mSIS and BG groups were not significant (P>0.05). All of these parameters were significantly different from those in the control group (P<0.05). Conclusions. These findings confirmed that mSIS combined with the bone substitute material enhanced bone regeneration in peri-implant defects, in a manner similar to that of a collagen membrane.

scholarly journals Collagenated Synthetic Bone Substitute Material for Sinus Floor Elevation at Sites with a Perforated Schneiderian Membrane

2020 ◽  
Vol 9 (11) ◽  
pp. 3764
Author(s):  
Sangyup Kim ◽  
Jong-Hyuk Chung ◽  
Seung-Yun Shin ◽  
Seung-Il Shin ◽  
Ji-Youn Hong ◽  
...  
Keyword(s):  
Bone Formation ◽  
Dimensional Stability ◽  
Bone Substitute ◽  
Control Group ◽  
New Bone Formation ◽  
Bone Substitute Material ◽  
Sinus Floor Elevation ◽  
Schneiderian Membrane ◽  
Sinus Floor ◽  
Substitute Material

Schneiderian membrane perforation (SMP) is the most common complication during sinus floor elevation (SFE). Conventional methods to repair SMP, such as using a collagen barrier, may be clinically demanding. The aim of the present study was to compare the effects of collagenated bone substitute materials with and without a collagen barrier to repair SMP during SFE in terms of new bone formation and dimensional stability. In 12 rabbits, intentional SMP was made during bilateral SFE. The rabbits were randomly assigned under two groups: the control group, in which the sinus was repaired with a collagen barrier, and the test group, in which the sinus was repaired without a collagen barrier. Collagenated bone substitute material was grafted in both groups. Healing periods of 2 weeks and 4 weeks were provided in both groups. There were no adverse clinical events. Histology revealed that the Schneiderian membrane had atrophied with loss of cilia and serous glands in both groups at 4 weeks. Histomorphometry revealed that the newly formed bone (test: 0.42 ± 0.17 mm2, control: 0.36 ± 0.18 mm2 at 2 weeks; test: 1.21 ± 0.36 mm2, control: 1.23 ± 0.55 mm2 at 4 weeks) or total augmented area did not significantly differ between the two groups at either time points (p > 0.05). In conclusion, collagenated bone substitute material without a collagen barrier demonstrated similar new bone formation and dimensional stability as that with a collagen barrier in repairing SMP.

Gelatine modified monetite as a bone substitute material: An in vitro assessment of bone biocompatibility

2016 ◽  
Vol 32 ◽  
pp. 275-285 ◽  
Author(s):  
Benjamin Kruppke ◽  
Jana Farack ◽  
Alena-Svenja Wagner ◽  
Sarah Beckmann ◽  
Christiane Heinemann ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Bone Substitute Material ◽  
In Vitro Assessment ◽  
Substitute Material

scholarly journals Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

2020 ◽  
Vol 35 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Solomiya Kyyak ◽  
Sebastian Blatt ◽  
Andreas Pabst ◽  
Daniel Thiem ◽  
Bilal Al-Nawas ◽  
...  
Keyword(s):  
Bone Substitute ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Substitute Material ◽  
Platelet Rich Fibrin ◽  
Osteoblast Activity ◽  
Substitute Material ◽  
Xenogenic Bone

The aim of the in vitro study was a comparison of an allogenic (ABSM) and a xenogenic bone substitute material (XBSM) with and without injectable platelet-rich fibrin (ABSM-i-PRF & XBSM-i-PRF) on cell characteristics of human osteoblasts (HOB). Here, ABSM and XBSM (+ i-PRF = test; - i-PRF = control) were incubated with HOB for 3, 7 and 10 days. HOB viability, migration, proliferation and differentiation (RT-PCR on alkaline phosphatase (AP), bone morphogenetic protein 2 (BMP-2) and osteonectin (OCN)) were measured and compared between groups. At day 3, an increased viability, migration and proliferation was seen for ABSM-i-PRF. For viability and proliferation (days 7 and 10) and for migration (day 10), ABSM-i-PRF/XBSM-i-PRF showed higher values compared to ABSM/XBSM with maximum values for ABSM-i-PRF and minimum values for XBSM. At days 3 and 7, the highest expression of AP was detected in ABSM-i-PRF/XBSM-i-PRF when compared to ABSM/XBSM, whereas at day 10, AP expression levels were elevated in ABSM-i-PRF/ABSM. The highest BMP-2 expression was seen in ABSM-i-PRF whereas OCN expression showed higher levels in ABSM-i-PRF/XBSM-i-PRF at days 3 and 7 with lowest expression for ABSM. Later on, elevated OC levels were detected for ABSM-i-PRF only. In conclusion, i-PRF in combination with ABSM enhances HOB activity when compared to XBSM-i-PRF or untreated BSM in vitro. Therefore, addition of i-PRF to ABSM and – to a lower extent – to XBSM may influence osteoblast activity in vivo.

In Vitro Antibacterial Properties of Vancomycin-Loaded Nano-Hydroxyapatite/Collagen/Calcium Sulfate Hemihydrates (VCM/nHAC/CSH) Bone Substitute

2013 ◽  
Vol 745-746 ◽  
pp. 6-12 ◽  
Author(s):  
Xiao Jie Lian ◽  
Xu Mei Wang ◽  
Fu Zhai Cui
Keyword(s):  
Bone Substitute ◽  
Bone Repair ◽  
Porous Scaffold ◽  
Setting Time ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Calcium Sulphate ◽  
Bone Graft Substitute ◽  
Potential Applications

In the present study, a new antibacterial bone graft substitute has been developed for repairing bone defects and inhibiting related infections at the same time. Calcium sulphate hemihydrate (CSH) was introduced into nanohydroxyapatite/collagen (nHAC) to prepare a self-settingin situbone repair materials. The nHAC/CSH was used as a carrier of vancomycin (VCM) for anti-infection and the treatment of osteomyelitis. The VCM/nHAC/CSH composite was a porous scaffold with porosity of 38.8% and the compressive mechanical strength was about 4.8 MPa. The final setting time was about 15~20 min. The inhibition ratio of VCM/nHAC/CSH was more than 99.8% and the distinct inhibition zone of 18 mm was formed in Staphylococcus aureus bacterium incubation dish with VCM/nHAC/CSH disc in the center of agar matrix for 16 hours of incubation. After incubating 17 days at 37in vitro, the concentration of vancomycin in elution fluild was around 12 μg/mL. Therefore, the VCM/nHAC/CSH bone substitute presents ideal self-setting antibacterial, cytocompatibility, sustained release properties and has great potential applications for the treatment of bone defect-related infection in orthopedic surgeries.

Study of Nano-Hydroxyapatite/Polycarbonate Composite for Bone Repair

2009 ◽  
Vol 620-622 ◽  
pp. 587-590
Author(s):  
Jian Guo Liao ◽  
Yu Bao Li ◽  
Li Zhang ◽  
Yi Zuo
Keyword(s):  
Bone Substitute ◽  
Bone Repair ◽  
Clinical Applications ◽  
Basic Requirement ◽  
Inorganic N ◽  
Bone Substitute Material ◽  
Novel Technique ◽  
Substitute Material ◽  
Physical And Chemical ◽  
Bone Apatite

Acicular nano-hydroxyapatite (n-HA) was used to make a new biomaterial composite with polycarbonate by a novel technique. The physical and chemical characteristics of the composites were tested. It was found that the synthesized n-HA crystals were similar to bone apatite in size, phase composition and crystal structure. The TEM results indicated the biomimetic n-HA crystals were uniformly distributed in the polymer matrix. Chemical inter-action between inorganic n-HA and polycarbonate was investigated and discussed. These results indicate that the composite fulfills the basic requirement of bone substitute material, and has the potential for clinical applications.

scholarly journals In Vitro and In Vivo Evaluation of Starfish Bone-Derived β-Tricalcium Phosphate as a Bone Substitute Material

Materials ◽  
2019 ◽  
Vol 12 (11) ◽  
pp. 1881 ◽  
Author(s):  
Haruka Ishida ◽  
Hisao Haniu ◽  
Akari Takeuchi ◽  
Katsuya Ueda ◽  
Mahoko Sano ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Bone Substitute ◽  
Fibrous Tissue ◽  
Bone Substitute Material ◽  
In Vivo Evaluation ◽  
Tissue Invasion ◽  
Substitute Material ◽  
Porous Calcium Carbonate

We evaluated starfish-derived β-tricalcium phosphate (Sf-TCP) obtained by phosphatization of starfish-bone-derived porous calcium carbonate as a potential bone substitute material. The Sf-TCP had a communicating pore structure with a pore size of approximately 10 μm. Although the porosity of Sf-TCP was similar to that of Cerasorb M (CM)—a commercially available β-TCP bone filler—the specific surface area was roughly three times larger than that of CM. Observation by scanning electron microscopy showed that pores communicated to the inside of the Sf-TCP. Cell growth tests showed that Sf-TCP improved cell proliferation compared with CM. Cells grown on Sf-TCP showed stretched filopodia and adhered; cells migrated both to the surface and into pores. In vivo, vigorous tissue invasion into pores was observed in Sf-TCP, and more fibrous tissue was observed for Sf-TCP than CM. Moreover, capillary formation into pores was observed for Sf-TCP. Thus, Sf-TCP showed excellent biocompatibility in vitro and more vigorous bone formation in vivo, indicating the possible applications of this material as a bone substitute. In addition, our findings suggested that mimicking the microstructure derived from whole organisms may facilitate the development of superior artificial bone.

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