In vitro study of viability and apoptosis of mesenchymal stem cells from stromal vascular fraction (SVF) in combination with bovine bone cancellous as a good bone substitute material

2019 ◽  
Author(s):  
Panji Sananta ◽  
Warih Anggoro Mustaqim ◽  
Sri Andarini ◽  
Respati Suryanto Dradjat
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Substitute ◽  
Stromal Vascular Fraction ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bovine Bone ◽  
Bone Substitute Material ◽  
Substitute Material

scholarly journals Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

2020 ◽  
Vol 35 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Solomiya Kyyak ◽  
Sebastian Blatt ◽  
Andreas Pabst ◽  
Daniel Thiem ◽  
Bilal Al-Nawas ◽  
...  
Keyword(s):  
Bone Substitute ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Substitute Material ◽  
Platelet Rich Fibrin ◽  
Osteoblast Activity ◽  
Substitute Material ◽  
Xenogenic Bone

The aim of the in vitro study was a comparison of an allogenic (ABSM) and a xenogenic bone substitute material (XBSM) with and without injectable platelet-rich fibrin (ABSM-i-PRF & XBSM-i-PRF) on cell characteristics of human osteoblasts (HOB). Here, ABSM and XBSM (+ i-PRF = test; - i-PRF = control) were incubated with HOB for 3, 7 and 10 days. HOB viability, migration, proliferation and differentiation (RT-PCR on alkaline phosphatase (AP), bone morphogenetic protein 2 (BMP-2) and osteonectin (OCN)) were measured and compared between groups. At day 3, an increased viability, migration and proliferation was seen for ABSM-i-PRF. For viability and proliferation (days 7 and 10) and for migration (day 10), ABSM-i-PRF/XBSM-i-PRF showed higher values compared to ABSM/XBSM with maximum values for ABSM-i-PRF and minimum values for XBSM. At days 3 and 7, the highest expression of AP was detected in ABSM-i-PRF/XBSM-i-PRF when compared to ABSM/XBSM, whereas at day 10, AP expression levels were elevated in ABSM-i-PRF/ABSM. The highest BMP-2 expression was seen in ABSM-i-PRF whereas OCN expression showed higher levels in ABSM-i-PRF/XBSM-i-PRF at days 3 and 7 with lowest expression for ABSM. Later on, elevated OC levels were detected for ABSM-i-PRF only. In conclusion, i-PRF in combination with ABSM enhances HOB activity when compared to XBSM-i-PRF or untreated BSM in vitro. Therefore, addition of i-PRF to ABSM and – to a lower extent – to XBSM may influence osteoblast activity in vivo.

Melt-Derived Condensed Polymorphic Calcium Phosphate as Bone Substitute Material: An In Vitro Study

2011 ◽  
Vol 94 (9) ◽  
pp. 3023-3029 ◽  
Author(s):  
Anbalagan Balamurugan ◽  
Khalil El Mabrouk ◽  
Sandra Pina ◽  
Mostapha M. Bousmina ◽  
Jose M. F. Ferreira
Keyword(s):  
Calcium Phosphate ◽  
Bone Substitute ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bone Substitute Material ◽  
Substitute Material

Comparing the osteogenic potential of schneiderian membrane and dental pulp mesenchymal stem cells: an in vitro study

2021 ◽  
Author(s):  
Antoine Berbéri ◽  
Joseph Sabbagh ◽  
Rita Bou Assaf ◽  
Michella Ghassibe-Sabbagh ◽  
Fatima Al-Nemer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Schneiderian Membrane

In vivo and in vitro study of osteogenic potency of endothelin-1 on bone marrow-derived mesenchymal stem cells

2017 ◽  
Vol 357 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Long-Wei Hu ◽  
Xiao Wang ◽  
Xin-Qun Jiang ◽  
Li-Qun Xu ◽  
Hong-Ya Pan
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Endothelin 1

The responses of human adipose-derived mesenchymal stem cells on polycaprolactone-based scaffolds: an in vitro study

2014 ◽  
Vol 11 (3) ◽  
pp. 239-246 ◽  
Author(s):  
Thanaphum Osathanon ◽  
Boontharika Chuenjitkuntaworn ◽  
Nunthawan Nowwarote ◽  
Pitt Supaphol ◽  
Panunn Sastravaha ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Study ◽  
Vitro Study

Retraction: Mesenchymal Stem Cells: Are They Suitable for Systemic Injection? An In Vitro Study

Angiology ◽  
2009 ◽  
Author(s):  
M. Ugurlucan ◽  
D. Furlani ◽  
L.-L. Ong ◽  
C. Yerebakan ◽  
W.-W. Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Systemic Injection

scholarly journals Ingrowth of human mesenchymal stem cells into porous silk particle reinforced silk composite scaffolds: An in vitro study

2011 ◽  
Vol 7 (1) ◽  
pp. 144-151 ◽  
Author(s):  
Danielle N. Rockwood ◽  
Eun Seok Gil ◽  
Sang-Hyug Park ◽  
Jonathan A. Kluge ◽  
Warren Grayson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Composite Scaffolds

Mesenchymal stem cells with osteogenic potential in human maxillary sinus membrane: an in vitro study

2016 ◽  
Vol 21 (5) ◽  
pp. 1599-1609 ◽  
Author(s):  
Antoine Berbéri ◽  
Fatima Al-Nemer ◽  
Eva Hamade ◽  
Ziad Noujeim ◽  
Bassam Badran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Maxillary Sinus ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Sinus Membrane
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