Incorporation of exudates of human platelet-rich fibrin gel in biodegradable fibrin scaffolds for tissue engineering of cartilage

2012 ◽  
Vol 100B (4) ◽  
pp. 948-955 ◽  
Author(s):  
Chi-Sheng Chien ◽  
Hsiu-O Ho ◽  
Yu-Chih Liang ◽  
Pai-Hung Ko ◽  
Ming-Thau Sheu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Human Platelet ◽  
Fibrin Gel ◽  
Platelet Rich Fibrin

Advanced Platelet-Rich Fibrin: A New Concept for Cell-Based Tissue Engineering by Means of Inflammatory Cells

2014 ◽  
Vol 40 (6) ◽  
pp. 679-689 ◽  
Author(s):  
Shahram Ghanaati ◽  
Patrick Booms ◽  
Anna Orlowska ◽  
Alica Kubesch ◽  
Jonas Lorenz ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
B Lymphocytes ◽  
Tissue Regeneration ◽  
Distal Part ◽  
T And B Lymphocytes ◽  
Cell Detection ◽  
Neutrophilic Granulocytes ◽  
Platelet Rich Fibrin ◽  
Study Protocols

Choukroun's platelet-rich fibrin (PRF) is obtained from blood without adding anticoagulants. In this study, protocols for standard platelet-rich fibrin (S-PRF) (2700 rpm, 12 minutes) and advanced platelet-rich fibrin (A-PRF) (1500 rpm, 14 minutes) were compared to establish by histological cell detection and histomorphometrical measurement of cell distribution the effects of the centrifugal force (speed and time) on the distribution of cells relevant for wound healing and tissue regeneration. Immunohistochemistry for monocytes, T and B -lymphocytes, neutrophilic granulocytes, CD34-positive stem cells, and platelets was performed on clots produced from four different human donors. Platelets were detected throughout the clot in both groups, although in the A-PRF group, more platelets were found in the distal part, away from the buffy coat (BC). T- and B-lymphocytes, stem cells, and monocytes were detected in the surroundings of the BC in both groups. Decreasing the rpm while increasing the centrifugation time in the A-PRF group gave an enhanced presence of neutrophilic granulocytes in the distal part of the clot. In the S-PRF group, neutrophils were found mostly at the red blood cell (RBC)-BC interface. Neutrophilic granulocytes contribute to monocyte differentiation into macrophages. Accordingly, a higher presence of these cells might be able to influence the differentiation of host macrophages and macrophages within the clot after implantation. Thus, A-PRF might influence bone and soft tissue regeneration, especially through the presence of monocytes/macrophages and their growth factors. The relevance and feasibility of this tissue-engineering concept have to be proven through in vivo studies.

Cartilage Scales Embedded in Fibrin Gel

2017 ◽  
Vol 33 (02) ◽  
pp. 225-232 ◽  
Author(s):  
Milos Kovacevic ◽  
Frank Riedel ◽  
Jochen Wurm ◽  
Gregor Bran
Keyword(s):  
Regenerative Medicine ◽  
Surgical Techniques ◽  
Fibrin Gel ◽  
Platelet Rich Fibrin ◽  
Preliminary Results ◽  
Advantages And Disadvantages ◽  
The Past ◽  
Dorsal Nasal Augmentation ◽  
Diced Cartilage ◽  
Nasal Augmentation

Multiple techniques have been described for dorsal nasal augmentation in rhinoplasty. In this article, we review common surgical techniques for raising the dorsum or eliminating dorsal irregularities, by highlighting inherent advantages and disadvantages of each method. Within the past few years, the use of diced cartilage grafts has become the workhorse in this field of interest. To overcome drawbacks of methods based on diced cartilage, we present a new concept for autologous augmentation, using regenerative medicine protocols. A mix of cartilage scales with cartilage pâté was embedded in platelet-rich fibrin (PRF). Since December 2015, a total of 48 patients were treated with this technique. Based on our preliminary results, cartilage scales in PRF appear to be a promising and reliable alternative to existing procedures for dorsal nasal augmentation.

Fibrin Gel as Alternative Scaffold for Respiratory Tissue Engineering

2011 ◽  
Vol 40 (3) ◽  
pp. 679-687 ◽  
Author(s):  
Christian G. Cornelissen ◽  
Maren Dietrich ◽  
Stefan Krüger ◽  
Jan Spillner ◽  
Thomas Schmitz-Rode ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fibrin Gel ◽  
Alternative Scaffold ◽  
Respiratory Tissue

scholarly journals Fibrin Gel as an Injectable Biodegradable Scaffold and Cell Carrier for Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Yuting Li ◽  
Hao Meng ◽  
Yuan Liu ◽  
Bruce P. Lee
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Polymer Network ◽  
Tissue Engineering Scaffold ◽  
Fibrin Gel ◽  
Tissue Engineering Scaffolds ◽  
Injectable Scaffolds ◽  
Synthetic Materials ◽  
Cell Carrier ◽  
A Cell

Due to the increasing needs for organ transplantation and a universal shortage of donated tissues, tissue engineering emerges as a useful approach to engineer functional tissues. Although different synthetic materials have been used to fabricate tissue engineering scaffolds, they have many limitations such as the biocompatibility concerns, the inability to support cell attachment, and undesirable degradation rate. Fibrin gel, a biopolymeric material, provides numerous advantages over synthetic materials in functioning as a tissue engineering scaffold and a cell carrier. Fibrin gel exhibits excellent biocompatibility, promotes cell attachment, and can degrade in a controllable manner. Additionally, fibrin gel mimics the natural blood-clotting process and self-assembles into a polymer network. The ability for fibrin to curein situhas been exploited to develop injectable scaffolds for the repair of damaged cardiac and cartilage tissues. Additionally, fibrin gel has been utilized as a cell carrier to protect cells from the forces during the application and cell delivery processes while enhancing the cell viability and tissue regeneration. Here, we review the recent advancement in developing fibrin-based biomaterials for the development of injectable tissue engineering scaffold and cell carriers.

Retina tissue engineering by conjunctiva mesenchymal stem cells encapsulated in fibrin gel: Hypotheses on novel approach to retinal diseases treatment

2017 ◽  
Vol 101 ◽  
pp. 75-77 ◽  
Author(s):  
Mostafa Soleimannejad ◽  
Somayeh Ebrahimi-Barough ◽  
Samad Nadri ◽  
Mohammad Riazi-Esfahani ◽  
Masoud Soleimani ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Retinal Diseases ◽  
Fibrin Gel ◽  
Novel Approach

scholarly journals Influence of platelet storage time on human platelet lysates and platelet lysate-expanded mesenchymal stromal cells for bone tissue engineering

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Siddharth Shanbhag ◽  
Samih Mohamed-Ahmed ◽  
Turid Helen Felli Lunde ◽  
Salwa Suliman ◽  
Anne Isine Bolstad ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Stromal Cells ◽  
Storage Time ◽  
Human Platelet ◽  
Scaling Up ◽  
Platelet Lysate ◽  
Lineage Differentiation

Abstract Background Human platelet lysate (HPL) is emerging as the preferred xeno-free supplement for the expansion of mesenchymal stromal cells (MSCs) for bone tissue engineering (BTE) applications. Due to a growing demand, the need for standardization and scaling-up of HPL has been highlighted. However, the optimal storage time of the source material, i.e., outdated platelet concentrates (PCs), remains to be determined. The present study aimed to determine the optimal storage time of PCs in terms of the cytokine content and biological efficacy of HPL. Methods Donor-matched bone marrow (BMSCs) and adipose-derived MSCs (ASCs) expanded in HPL or fetal bovine serum (FBS) were characterized based on in vitro proliferation, immunophenotype, and multi-lineage differentiation. Osteogenic differentiation was assessed at early (gene expression), intermediate [alkaline phosphatase (ALP) activity], and terminal stages (mineralization). Using a multiplex immunoassay, the cytokine contents of HPLs produced from PCs stored for 1–9 months were screened and a preliminary threshold of 4 months was identified. Next, HPLs were produced from PCs stored for controlled durations of 0, 1, 2, 3, and 4 months, and their efficacy was compared in terms of cytokine content and BMSCs’ proliferation and osteogenic differentiation. Results BMSCs and ASCs in both HPL and FBS demonstrated a characteristic immunophenotype and multi-lineage differentiation; osteogenic differentiation of BMSCs and ASCs was significantly enhanced in HPL vs. FBS. Multiplex network analysis of HPL revealed several interacting growth factors, chemokines, and inflammatory cytokines. Notably, stem cell growth factor (SCGF) was detected in high concentrations. A majority of cytokines were elevated in HPLs produced from PCs stored for ≤ 4 months vs. > 4 months. However, no further differences in PC storage times between 0 and 4 months were identified in terms of HPLs’ cytokine content or their effects on the proliferation, ALP activity, and mineralization of BMSCs from multiple donors. Conclusions MSCs expanded in HPL demonstrate enhanced osteogenic differentiation, albeit with considerable donor variation. HPLs produced from outdated PCs stored for up to 4 months efficiently supported the proliferation and osteogenic differentiation of MSCs. These findings may facilitate the standardization and scaling-up of HPL from outdated PCs for BTE applications.

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