The characterization and optimization of injectable silicone resin particles in conjunction with dermal fibroblasts and growth factors: Anin vitrostudy

2010 ◽  
Vol 9999B ◽  
pp. NA-NA
Author(s):  
Robert M. Crews ◽  
Jessica A. Jennings ◽  
Jonathan McCanless ◽  
Judith A. Cole ◽  
Joel D. Bumgardner ◽  
...  
Keyword(s):  
Growth Factors ◽  
Dermal Fibroblasts ◽  
Silicone Resin

Effect of growth factors in combination with injectable silicone resin particles on the biological activity of dermal fibroblasts: A preliminaryin vitrostudy

2010 ◽  
Vol 92B (1) ◽  
pp. 255-260 ◽  
Author(s):  
Jessica A. Jennings ◽  
Robert M. Crews ◽  
Joycelyn Robinson ◽  
Kelly Richelsoph ◽  
Judith A. Cole ◽  
...  
Keyword(s):  
Biological Activity ◽  
Growth Factors ◽  
Dermal Fibroblasts ◽  
Silicone Resin

scholarly journals Efeito do ácido valpróico e dos fatores de crescimento na plasticidade dos fibroblastos dérmicos felinos / Effect of Valproic acid and growth factors on plasticity of feline dermal fibroblasts

2021 ◽  
Vol 4 (3) ◽  
pp. 2889-2901
Author(s):  
Diana Maritza Echeverry Berrío ◽  
Constanza Javiera Aguilera González ◽  
Daniela Michel Rojas Mansilla ◽  
Lleretny Rodríguez- Álvarez ◽  
Fidel Ovidio Castro
Keyword(s):  
Valproic Acid ◽  
Growth Factors ◽  
Dermal Fibroblasts ◽  
De Se

As células-tronco mesenquimais (CTM) são utilizadas na terapia celular, isolando-se de diferentes tecidos, incluindo a pele. Os fibroblastos dérmicos têm mostrado características de potência semelhantes às CTMs como expressão de marcadores de superfície e diferenciação para outros tipos de linhagens de origem mesodérmica, principalmente sob a influência de moduladores epigenéticos e fatores de crescimento. A terapia celular em medicina veterinária, especificamente em gatos, representa um desafio devido à invasividade na obtenção de tecidos fonte de CTM, para os quais outras opções menos invasivas estão sendo buscadas para an obtenção dessas células. O objetivo deste estudo foi avaliar o efeito do ácido valpróico - VPA (modulador epigenético) e fatores de crescimento (PRP e h-PDGF-B) na expressão de marcadores de superfície, genes de pluripotência e capacidade de diferenciação mesodérmica de fibroblastos felinos. Fibroblastos de pele foram isolados de gatas e cultivados com VPA e fatores de crescimento por 12 dias. A expressão de Cd90, Cd44, E-Caderina, Snail, Nanog e Oct4 foi avaliada nos dias 5 e 12. O potencial de diferenciação adipogênico, condrogênico e osteogênico dos fibroblastos foi avaliado após 12 dias de tratamento. A expressão de Cd44 aumentou no dia 5 do tratamento com VPA + PRP (p = 0,01). A expressão de Oct4 e Nanog aumentou no dia 5 do tratamento com VPA + h-PDGF-B (p 0,05). Fibroblastos em tratamento com VPA e h-PDGF-B mostraram capacidade de se diferenciar para condrogênese e osteogênese. O protocolo de cultura de células para fibroblastos felinos com VPA e h-PDGF-B confere plasticidade aos fibroblastos felinos ao promover a expressão de Nanog e Oct4, bem como a diferenciação mesodérmica in vitro.

Response to growth factors of human dermal fibroblasts in a quiescent state owing to cell-matrix contact inhibition

Matrix ◽  
1991 ◽  
Vol 11 (2) ◽  
pp. 71-75 ◽  
Author(s):  
Toshio Nishiyama ◽  
Nobuko Akutsu ◽  
Izumi Horii ◽  
Yasuhisa Nakayama ◽  
Tatsuya Ozawa ◽  
...  
Keyword(s):  
Growth Factors ◽  
Dermal Fibroblasts ◽  
Contact Inhibition ◽  
Quiescent State ◽  
Human Dermal Fibroblasts ◽  
Cell Matrix

scholarly journals Enhanced Platelet-Rich Plasma (ePRP) Stimulates Wound Healing through Effects on Metabolic Reprogramming in Fibroblasts

2021 ◽  
Vol 22 (23) ◽  
pp. 12623
Author(s):  
Hsin-Pei Weng ◽  
Yuan-Yang Cheng ◽  
Hsin-Lun Lee ◽  
Tai-Yi Hsu ◽  
Yu-Tang Chang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Energy Metabolism ◽  
Metabolic Regulation ◽  
Platelet Rich Plasma ◽  
Maxillofacial Surgery ◽  
Dermal Fibroblasts ◽  
Metabolic Reprogramming ◽  
Metabolic Effects ◽  
Oral And Maxillofacial Surgery

As a source of growth factors for expediting wound healing and tissue regeneration, plasma-rich plasma (PRP) has been extensively applied in diverse fields including orthopaedics, ophthalmology, oral and maxillofacial surgery, dentistry, and gynaecology. However, the function of PRP in metabolic regulations remains enigmatic. A standardized method was devised herein to enrich growth factors and to lyophilize it as enhanced PRP (ePRP) powder, which could become ubiquitously available without mechanical centrifugation in clinical practice. To identify metabolic reprogramming in human dermal fibroblasts under ePRP treatment, putative metabolic targets were identified by transcriptome profiling and validated for their metabolic effects and mechanism. ePRP does not only promote wound healing but re-aligns energy metabolism by shifting to glycolysis through stimulation of glycolytic enzyme activity in fibroblasts. On the contrary, oxygen consumption rates and several mitochondrial respiration activities were attenuated in ePRP-treated fibroblasts. Furthermore, ePRP treatment drives the mitochondrial resetting by hindering the mitochondrial biogenesis-related genes and results in a dampened mitochondrial mass. Antioxidant production was further increased by ePRP treatment to prevent reactive oxygen species formation. Besides, ePRP also halts the senescence progression of fibroblasts by activating SIRT1 expression. Importantly, the glycolytic inhibitor 2-DG can completely reverse the ePRP-enhanced wound healing capacity, whereas the mitochondrial inhibitor oligomycin cannot. This is the first study to utilize PRP for comprehensively investigating its effects on the metabolic reprogramming of fibroblasts. These findings indicate that PRP’s primary metabolic regulation is to promote metabolic reprogramming toward glycolytic energy metabolism in fibroblasts, preserving redox equilibrium and allowing anabolic pathways necessary for the healing and anti-ageing process.

scholarly journals Reduced growth of dermal fibroblasts from chronic venous ulcers can be stimulated with growth factors

1997 ◽  
Vol 26 (6) ◽  
pp. 994-1001 ◽  
Author(s):  
Andrew C. Stanley ◽  
Hee-Young Park ◽  
Tania J. Phillips ◽  
Vladimir Russakovsky ◽  
James O. Menzoian
Keyword(s):  
Growth Factors ◽  
Dermal Fibroblasts ◽  
Venous Ulcers

Effect of transforming growth factors-β on collagen type VI expression in human dermal fibroblasts

FEBS Letters ◽  
1992 ◽  
Vol 310 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Marc Heckmann ◽  
Monique Aumailley ◽  
Mon-Li Chu ◽  
Rupert Timpl ◽  
Thomas Krieg
Keyword(s):  
Growth Factors ◽  
Collagen Type ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Transforming Growth Factors ◽  
Collagen Type Vi
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