Effect of growth factors in combination with injectable silicone resin particles on the biological activity of dermal fibroblasts: A preliminaryin vitrostudy

2010 ◽  
Vol 92B (1) ◽  
pp. 255-260 ◽  
Author(s):  
Jessica A. Jennings ◽  
Robert M. Crews ◽  
Joycelyn Robinson ◽  
Kelly Richelsoph ◽  
Judith A. Cole ◽  
...  
Keyword(s):  
Biological Activity ◽  
Growth Factors ◽  
Dermal Fibroblasts ◽  
Silicone Resin

The characterization and optimization of injectable silicone resin particles in conjunction with dermal fibroblasts and growth factors: Anin vitrostudy

2010 ◽  
Vol 9999B ◽  
pp. NA-NA
Author(s):  
Robert M. Crews ◽  
Jessica A. Jennings ◽  
Jonathan McCanless ◽  
Judith A. Cole ◽  
Joel D. Bumgardner ◽  
...  
Keyword(s):  
Growth Factors ◽  
Dermal Fibroblasts ◽  
Silicone Resin

scholarly journals Microenvironment Influence of a Novel Bioengineered Wound Product, APIS®: A Preliminary In Vitro Analysis of Inflammatory Marker and Growth Factor Secretion

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Isaac Rodriguez ◽  
Tricia Conti ◽  
Nina Bionda
Keyword(s):  
Biological Activity ◽  
Growth Factors ◽  
Growth Factor ◽  
Chronic Wounds ◽  
Inflammatory Marker ◽  
Protein Levels ◽  
Vitro Analysis ◽  
Related Proteins ◽  
Endothelial Growth Factor

Objective. Preliminary biological activity assessment of a novel bioengineered wound product (APIS®, SweetBio, Inc., Memphis, TN, USA), a synthesis of gelatin, Manuka honey, and hydroxyapatite, with in vitro indications to protect, instill balance to, and progress the wound microenvironment. Approach. The biological activity the bioengineered wound product (BWP) elicits on human cells in vitro was assessed by evaluating matrix metalloproteinase- (MMP-) related proteins expressed by macrophages and secretion of growth factors in fibroblasts. Cells were cultured with no treatment, stimulated with lipopolysaccharides (LPS), or seeded directly on the BWP for 24 hours. An additional 72-hour time point for the BWP was assessed to determine if the BWP maintained its activity compared to itself at 24 hours. Cell culture supernatants were assayed to quantify secreted protein levels. Results. MMP-9 secretion from macrophages seeded on the BWP were nondetectable ( P < 0.01 ), while a tissue inhibitor of MMP (TIMP-1) was detected. This decreased the overall MMP-9/TIMP-1 ratio secreted from macrophages seeded on the BWP compared to the controls. Additionally, the secretion of prohealing growth factors such as basic fibroblast growth factor (FGFb) and vascular endothelial growth factor (VEGF) was observed. Conclusion. Results from this preliminary in vitro evaluation suggest that the BWP has the potential to instill balance to the wound microenvironment by reducing the MMP-9/TIMP-1 ratio secretion from macrophages and progress previously stalled chronic wounds towards healing by triggering the release of growth factors from fibroblasts.

scholarly journals Dual Asymmetric Centrifugation Efficiently Produces a Poloxamer-Based Nanoemulsion Gel for Topical Delivery of Pirfenidone

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Eugene P. Chung ◽  
Adrienne R. Wells ◽  
Mia Mae Kiamco ◽  
Kai P. Leung
Keyword(s):  
Biological Activity ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Release Kinetics ◽  
Dermal Fibroblasts ◽  
Poloxamer 407 ◽  
Batch Size ◽  
Formulation Development ◽  
Relative Standard ◽  
Nanoemulsion Gel

Abstract This study used dual asymmetric centrifugation (DAC) to produce a topical vehicle for Pirfenidone (Pf; 5-methyl-1-phenyl-2[1H]-pyridone)—a Food and Drug Administration-approved antifibrotic drug indicated for idiopathic fibrosis treatment. Pf was loaded (8 wt%) in a poloxamer nanoemulsion gel (PNG) formulation consisting of water (47.8 wt%), triacetin (27.6 wt%), poloxamer 407 (P407, 13.8 wt%), polysorbate 80 (1.8 wt%), and benzyl alcohol (0.9 wt%). To our knowledge, poloxamer gels are typically processed with either high-shear methods or temperature regulation and have not been emulsified using DAC. Using a single-step emulsification process, 2 min mixed at 2500 RPM resulted in the lowest Pf loading variability with a relative standard deviation (RSD) of 0.96% for a 1.5 g batch size. Batch sizes of 15 g and 100 g yield higher RSD of 4.18% and 3.05%, respectively, but still in compliance with USP guidelines. Ex vivo permeation in full thickness porcine skin after 24 h showed total Pf permeation of 404.90 ± 67.07 μg/cm2. Tested in vitro on human dermal fibroblasts stimulated with transforming growth factor-beta 1 (TGF-β1), Pf-PNG resulted in a > 2 fold decrease in α-SMA expression over vehicle control demonstrating that formulated Pf retained its biological activity. One-month stability testing at 25°C/60% relative humidity (RH) and 40°C/75% RH showed that % drug content, release kinetics, and biological activity were largely unchanged for both conditions; however, pH decreased from 6.7 to 5.5 (25°C/60% RH) and 4.5 (40°C/75% RH) after 1 month. Overall, these data demonstrate the utility of DAC to rapidly and reproducibly prepare lab-scale batches of emulsified gels for pharmaceutical formulation development.

scholarly journals Glycosaminoglycan-Inspired Biomaterials for the Development of Bioactive Hydrogel Networks

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 978 ◽  
Author(s):  
Mariana I. Neves ◽  
Marco Araújo ◽  
Lorenzo Moroni ◽  
Ricardo M.P. da Silva ◽  
Cristina C. Barrias
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Biological Activity ◽  
Growth Factors ◽  
Mechanical Stability ◽  
Biological Molecules ◽  
Inhibiting Factors ◽  
Wide Range ◽  
Different Sources

Glycosaminoglycans (GAG) are long, linear polysaccharides that display a wide range of relevant biological roles. Particularly, in the extracellular matrix (ECM) GAG specifically interact with other biological molecules, such as growth factors, protecting them from proteolysis or inhibiting factors. Additionally, ECM GAG are partially responsible for the mechanical stability of tissues due to their capacity to retain high amounts of water, enabling hydration of the ECM and rendering it resistant to compressive forces. In this review, the use of GAG for developing hydrogel networks with improved biological activity and/or mechanical properties is discussed. Greater focus is given to strategies involving the production of hydrogels that are composed of GAG alone or in combination with other materials. Additionally, approaches used to introduce GAG-inspired features in biomaterials of different sources will also be presented.

scholarly journals Integrated printed BDNF/collagen/chitosan scaffolds with low temperature extrusion 3D printer accelerated neural regeneration after spinal cord injury

2021 ◽  
Author(s):  
Xiao-Yin Liu ◽  
Chong Chen ◽  
Hai-Huan Xu ◽  
Yu-sheng Zhang ◽  
Lin Zhong ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Biological Activity ◽  
Growth Factors ◽  
3D Printing ◽  
Low Temperature ◽  
Neural Regeneration ◽  
Injury Site ◽  
Locomotor Function ◽  
Cord Injury ◽  
Chitosan Scaffolds

Abstract Recent studies have shown that 3D printed scaffolds integrated with growth factors can guide the growth of neurites and promote axon regeneration at the injury site. However, heat, organic solvents or cross-linking agents used in conventional 3D printing reduce the biological activity of growth factors. Low temperature 3D printing can incorporate growth factors into the scaffold and maintain their biological activity. In this study, we developed a collagen/chitosan scaffold integrated with brain-derived neurotrophic factor (3D-CC-BDNF) by low temperature extrusion 3D printing as a new type of artificial controlled release system, which could prolong the release of BDNF for the treatment of SCI. 8 weeks after the implantation of scaffolds in the transected lesion of T10 of the spinal cord, 3D-CC-BDNF significantly ameliorate locomotor function of the rats. Consistent with the recovery of locomotor function, 3D-CC-BDNF treatment could fill the gap, facilitate nerve fiber regeneration, accelerate the establishment of synaptic connections and enhance remyelination at the injury site.

scholarly journals Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts

2017 ◽  
Vol 13 (51) ◽  
pp. 462 ◽  
Author(s):  
Palanisamy Arulselvan ◽  
Sivapragasam Gothai ◽  
Katyakyini Muniandy ◽  
MazniAbu Zarin ◽  
TanWoan Sean ◽  
...  
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Ethyl Acetate ◽  
Moringa Oleifera ◽  
Ethyl Acetate Fraction ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

scholarly journals Efeito do ácido valpróico e dos fatores de crescimento na plasticidade dos fibroblastos dérmicos felinos / Effect of Valproic acid and growth factors on plasticity of feline dermal fibroblasts

2021 ◽  
Vol 4 (3) ◽  
pp. 2889-2901
Author(s):  
Diana Maritza Echeverry Berrío ◽  
Constanza Javiera Aguilera González ◽  
Daniela Michel Rojas Mansilla ◽  
Lleretny Rodríguez- Álvarez ◽  
Fidel Ovidio Castro
Keyword(s):  
Valproic Acid ◽  
Growth Factors ◽  
Dermal Fibroblasts ◽  
De Se

As células-tronco mesenquimais (CTM) são utilizadas na terapia celular, isolando-se de diferentes tecidos, incluindo a pele. Os fibroblastos dérmicos têm mostrado características de potência semelhantes às CTMs como expressão de marcadores de superfície e diferenciação para outros tipos de linhagens de origem mesodérmica, principalmente sob a influência de moduladores epigenéticos e fatores de crescimento. A terapia celular em medicina veterinária, especificamente em gatos, representa um desafio devido à invasividade na obtenção de tecidos fonte de CTM, para os quais outras opções menos invasivas estão sendo buscadas para an obtenção dessas células. O objetivo deste estudo foi avaliar o efeito do ácido valpróico - VPA (modulador epigenético) e fatores de crescimento (PRP e h-PDGF-B) na expressão de marcadores de superfície, genes de pluripotência e capacidade de diferenciação mesodérmica de fibroblastos felinos. Fibroblastos de pele foram isolados de gatas e cultivados com VPA e fatores de crescimento por 12 dias. A expressão de Cd90, Cd44, E-Caderina, Snail, Nanog e Oct4 foi avaliada nos dias 5 e 12. O potencial de diferenciação adipogênico, condrogênico e osteogênico dos fibroblastos foi avaliado após 12 dias de tratamento. A expressão de Cd44 aumentou no dia 5 do tratamento com VPA + PRP (p = 0,01). A expressão de Oct4 e Nanog aumentou no dia 5 do tratamento com VPA + h-PDGF-B (p 0,05). Fibroblastos em tratamento com VPA e h-PDGF-B mostraram capacidade de se diferenciar para condrogênese e osteogênese. O protocolo de cultura de células para fibroblastos felinos com VPA e h-PDGF-B confere plasticidade aos fibroblastos felinos ao promover a expressão de Nanog e Oct4, bem como a diferenciação mesodérmica in vitro.

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