Effect of surface topography on in vitro osteoblast function and mechanical performance of 3D printed titanium

2021 ◽  
Author(s):  
Bijan Abar ◽  
Cambre Kelly ◽  
Anh Pham ◽  
Nicholas Allen ◽  
Helena Barber ◽  
...  
Keyword(s):  
Surface Topography ◽  
Mechanical Performance ◽  
Osteoblast Function ◽  
3D Printed ◽  
Effect Of Surface

Improved osseointegration of 3D printed Ti-6Al-4V implant with a hierarchical micro/nano surface topography: An in vitro and in vivo study

2021 ◽  
Vol 118 ◽  
pp. 111505
Author(s):  
Bing Ren ◽  
Yi Wan ◽  
Chao Liu ◽  
Hongwei Wang ◽  
Mingzhi Yu ◽  
...  
Keyword(s):  
Surface Topography ◽  
In Vivo Study ◽  
3D Printed

scholarly journals The effect of surface topography and porosity on the tensile fatigue of 3D printed Ti-6Al-4V fabricated by selective laser melting

2019 ◽  
Vol 98 ◽  
pp. 726-736 ◽  
Author(s):  
Cambre N. Kelly ◽  
Nathan T. Evans ◽  
Cameron W. Irvin ◽  
Savita C. Chapman ◽  
Ken Gall ◽  
...  
Keyword(s):  
Surface Topography ◽  
Selective Laser Melting ◽  
Laser Melting ◽  
Tensile Fatigue ◽  
3D Printed ◽  
Effect Of Surface

1234 Evaluation of effect of surface topography on fixation of artificial joint in vitro

2005 ◽  
Vol 2005.5 (0) ◽  
pp. 209-210
Author(s):  
Kenichi YAMASAKI ◽  
Koji HATTORI ◽  
Hideo KONDO ◽  
Shigehiro HASHIMOTO ◽  
Yoshinori TAKAKURA ◽  
...  
Keyword(s):  
Surface Topography ◽  
Artificial Joint ◽  
Effect Of Surface

scholarly journals 4464 Effect of Surface Topography on In Vitro and Mechanical Performance of 3D Printed Titanium

2020 ◽  
Vol 4 (s1) ◽  
pp. 130-130
Author(s):  
Bijan Abar ◽  
Cambre Kelly ◽  
Anh Pham ◽  
Nicholas Allen ◽  
Helena Barber ◽  
...  
Keyword(s):  
Cell Growth ◽  
Tensile Testing ◽  
Mechanical Performance ◽  
Quantitative Polymerase Chain Reaction ◽  
Fluorescent Microscopy ◽  
Cell Activity ◽  
Implant Fixation ◽  
Cell Viability Assay ◽  
3D Printed

OBJECTIVES/GOALS: The goal of the study is to understand how changing the surface roughness of 3D printed Titanium either by processing printed samples or artificially printing rough topography impacts the mechanical and biological properties of the Titanium. METHODS/STUDY POPULATION: Titanium dog bones and discs were printed via laser powder bed fusion. groups were defined as 1. polished, 2.blasted, 4.as built, 4.sprouts and 5.rough sprouts. Roughness was measured with line measurement using a confocal microscope. Tensile testing of dog bones produced stress strain curves. MC3T3 preosteoblast were seeded on discs. Samples were analyzed at 0, 2, and 4 weeks. A cell viability assay and confocal fluorescent microscopy assessed cell growth. Alkaline Phosphatase (ALP) assay and Quantitative Polymerase Chain Reaction (qPCR) examined cell differentiation. Extracellular matrix (ECM) was stained for collagen and calcium. Scanning Electron Microcopy (SEM) was done on sputter coated discs. RESULTS/ANTICIPATED RESULTS: Measured roughness defined by Rz, maximum peak to valley distance of the sample profile ranged from 2.6-65.1 µm. The addition of printed roughness in the sprouts and rough sprouts group significantly diminished ductility resulting in early strain to failure during tensile testing. Cells adhered and proliferated on discs regardless of roughness group. There was no statistical difference in ALP activity, but qPCR showed that rough groups (sprouts and rough sprouts) had diminished Osteocalcin gene expression at week 2 and 4. The ECM in the rough groups was more resistant to repeated washes and was more extensive with SEM. DISCUSSION/SIGNIFICANCE OF IMPACT: Printing roughness diminished mechanical properties without clear benefit to cell growth. Roughness features were on mesoscale, unlike samples in literature on microscale that increase cell activity. Printed topography may aid in implant fixation and not osseous integration as hypothesized. CONFLICT OF INTEREST DESCRIPTION: Dr. Samual Adams, Dr. Ken Gall and Cambre Kelly own stock and/or stock options in restor3d, Inc.

Cytotoxicity and Adhesion Evaluation of Nanothickness Ca/P-Based Bioceramics Coated Titanium

2008 ◽  
Vol 396-398 ◽  
pp. 319-322
Author(s):  
Caroline M. Ramirez ◽  
Paulo Guilherme Coelho ◽  
José Mauro Granjeiro
Keyword(s):  
Cell Adhesion ◽  
Surface Topography ◽  
Dental Implants ◽  
Biological Response ◽  
Biological Evaluation ◽  
Titanium Implants ◽  
3T3 Fibroblasts ◽  
Plasma Sprayed ◽  
Effect Of Surface

Incorporation of bioceramics on the surface of dental implants has been utilized in an attempt to increase biological response of bone to materials. This paper reports the in vitro biological evaluation of Ca/P-based nanothickness bioceramic coated alumina-blasted/acid-etched titanium implants (AB/AE nanotite implant) and compare its performance to the untreated and uncoated implants, Ca/P-based nanothickness bioceramic coated untreated implants (untreated nanotite implant), alumina-blasted/acid-etched titanium implants (AB/AE implant) and hydroxyapatite plasma-sprayed implants (PSHA Implant). Balb/c 3T3 fibroblasts were used to asses the cytocompatibility of implant materials according to ISO-10993-5 protocols. Osteoblasts from Balb/c femurs seeded onto different implant surfaces showed the effect of surface topography and chemistry on cell adhesion. The results showed that all implants were not cytotoxic and that PSHA and AB/AE nanotite implants favored osteoblasts adhesion.

scholarly journals Polymer/Iron-Based Layered Double Hydroxides as Multifunctional Wound Dressings

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1130
Author(s):  
Mariana Pires Figueiredo ◽  
Ana Borrego-Sánchez ◽  
Fátima García-Villén ◽  
Dalila Miele ◽  
Silvia Rossi ◽  
...  
Keyword(s):  
Drug Release ◽  
High Performance ◽  
Mechanical Performance ◽  
Release Kinetics ◽  
Wound Dressings ◽  
In Vitro Drug Release ◽  
Improved Performance ◽  
Double Hydroxide ◽  
Double Hydroxides

This work presents the development of multifunctional therapeutic membranes based on a high-performance block copolymer scaffold formed by polyether (PE) and polyamide (PA) units (known as PEBA) and layered double hydroxide (LDH) biomaterials, with the aim to study their uses as wound dressings. Two LDH layer compositions were employed containing Mg2+ or Zn2+, Fe3+ and Al3+ cations, intercalated with chloride anions, abbreviated as Mg-Cl or Zn-Cl, or intercalated with naproxenate (NAP) anions, abbreviated as Mg-NAP or Zn-NAP. Membranes were structurally and physically characterized, and the in vitro drug release kinetics and cytotoxicity assessed. PEBA-loading NaNAP salt particles were also prepared for comparison. Intercalated NAP anions improved LDH–polymer interaction, resulting in membranes with greater mechanical performance compared to the polymer only or to the membranes containing the Cl-LDHs. Drug release (in saline solution) was sustained for at least 8 h for all samples and release kinetics could be modulated: a slower, an intermediate and a faster NAP release were observed from membranes containing Zn-NAP, NaNAP and Mg-NAP particles, respectively. In general, cell viability was higher in the presence of Mg-LDH and the membranes presented improved performance in comparison with the powdered samples. PEBA containing Mg-NAP sample stood out among all membranes in all the evaluated aspects, thus being considered a great candidate for application as multifunctional therapeutic dressings.

scholarly journals Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Selva Bilge ◽  
Emre Ergene ◽  
Ebru Talak ◽  
Seyda Gokyer ◽  
Yusuf Osman Donar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Carbonaceous Material ◽  
Hydrothermal Carbonization ◽  
Skeletal Muscle Tissue ◽  
Myotube Formation ◽  
3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

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