Comparison of visible and UVA phototoxicity in neural culture systems micropatterned with digital projection photolithography

2018 ◽  
Vol 107 (1) ◽  
pp. 134-144 ◽  
Author(s):  
Parastoo Khoshakhlagh ◽  
Devon A. Bowser ◽  
J. Quincy Brown ◽  
Michael J. Moore
Keyword(s):  
Culture Systems ◽  
Neural Culture ◽  
Digital Projection

Alterations of ultrastructure and elemental composition in cultured human epidermal keratinocytes after treatment with nitrofurazone and silver sulfadiazine

1987 ◽  
Vol 45 ◽  
pp. 676-677
Author(s):  
A. R. Crooker ◽  
M. C. Myers ◽  
T. L. Beard ◽  
E. S. Graham
Keyword(s):  
Electron Microscopy ◽  
Elemental Composition ◽  
Pyrolytic Carbon ◽  
Human Keratinocytes ◽  
Silver Sulfadiazine ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Culture Systems ◽  
Cytotoxicity Endpoints

Cell culture systems have become increasingly popular as a means of screening toxic agents and studying toxic mechanisms of drugs and other chemicals at the cellular and subcellular levels. These in vitro tests can be conducted rapidly in a broad range of relevant mammalian culture systems; a variety of biological and biochemical cytotoxicity endpoints can be examined. The following study utilized human keratinocytes to evaluate the relative cytotoxicities of nitrofurazone (NF) and silver sulfadiazine (SS), the active ingredients of FURACIN(R) Topical Cream and SILVADENE(R) Cream, respectively. These compounds are anti-infectives used in the treatment of burn patients. Cell ultrastructure and elemental composition were utilized as cytotoxicity endpoints.Normal Human Epidermal Keratinocytes (HK) were prepared from the EpiPackTM culture system (Clonetics Corporation, Boulder, CO). For scanning electron microscopy (SEM) and transmission electron microscopy (TEM), cells were seeded on sterile 35 mm Falcon plastic dishes; for elemental microanalysis, cells were plated on polished pyrolytic carbon discs (E. Fullam, Latham, NY) placed in the culture dishes.

Use of 3D culture systems to generate human induced pluripotent stem cell-derived [beta]-cells in vitro

2018 ◽  
Author(s):  
Fantuzzi Federica ◽  
Toivonen Sanna ◽  
Schiavo Andrea Alex ◽  
Pachera Nathalie ◽  
Rajaei Bahareh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Beta Cells ◽  
Pluripotent Stem Cell ◽  
3D Culture ◽  
Induced Pluripotent Stem Cell ◽  
Culture Systems ◽  
Induced Pluripotent

Protected Culture Systems for Strawberry Production

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 459d-459
Author(s):  
Fumiomi Takeda ◽  
Paul R. Adler ◽  
D. Michael Glenn
Keyword(s):  
Light Intensity ◽  
Fruit Size ◽  
Fruit Production ◽  
Hydroponic Culture ◽  
Production Cycle ◽  
Soilless Culture ◽  
Labor Costs ◽  
Culture Systems ◽  
Nutrient Film Technique ◽  
Plant Densities

Strawberry plants (cvs. Camarosa, Chandler, Sweet Charlie, Primetime, Jewel, and Tribute) were grown in soilless culture systems in a greenhouse from October to May. Fresh-dug and runner-tip Aplug® plants were transplanted into two systems: vertically stacked pots (24 plants/m2) containing perlite and horizontal nutrient film technique troughs (13 plants/m2). Plants were fertigated continuously with recirculating nutrient solution. In a 7-month production cycle, the plug plants bloomed earlier and produced more fruit during the first month of harvest (December) than the fresh-dug plants. Higher yields from plug plants were a result of more fruit numbers and not larger fruit size. Fruit production averaged 6.0 and 3.5 kg/m2 in the trough and pot systems, respectively. The vertical growing system allows greater plant densities, but light intensity reaching the plants in the lower sections of the tower can be less than 20% of levels measured at the top. Establishment costs of protected culture systems are higher, but production is earlier and labor costs are typically reduced. Greenhouse hydroponic culture systems could extend the winter strawberry production to more northern locations.

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