Polydopamine-collagen complex to enhance the biocompatibility of polydimethylsiloxane substrates for sustaining long-term culture of L929 fibroblasts and tendon stem cells

2017 ◽  
Vol 106 (2) ◽  
pp. 408-418 ◽  
Author(s):  
Qian Li ◽  
Lihong Sun ◽  
Lei Zhang ◽  
Zhigang Xu ◽  
Yuejun Kang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Long Term Culture ◽  
Tendon Stem Cells

scholarly journals Long term culture and differentiation of endothelial progenitor like cells from rat adipose derived stem cells

2017 ◽  
Vol 70 (1) ◽  
pp. 397-413 ◽  
Author(s):  
Monire Amerion ◽  
Mojtaba Rezazadeh Valojerdi ◽  
Saeid Abroun ◽  
Mehdi Totonchi
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Endothelial Progenitor ◽  
Long Term Culture

scholarly journals DNA Methylation Changes duringIn VitroPropagation of Human Mesenchymal Stem Cells: Implications for Their Genomic Stability?

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Angela Bentivegna ◽  
Mariarosaria Miloso ◽  
Gabriele Riva ◽  
Dana Foudah ◽  
Valentina Butta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ex Vivo ◽  
Genomic Stability ◽  
Great Promise ◽  
Human Mscs ◽  
Low Oxygen ◽  
Long Term Culture ◽  
Functional Changes

Mesenchymal stem cells (MSCs) hold great promise for the treatment of numerous diseases. A major problem for MSC therapeutic use is represented by the very low amount of MSCs which can be isolated from different tissues; thusex vivoexpansion is indispensable. Long-term culture, however, is associated with extensive morphological and functional changes of MSCs. In addition, the concern that they may accumulate stochastic mutations which lead the risk of malignant transformation still remains. Overall, the genome of human MSCs (hMSCs) appears to be apparently stable throughout culture, though transient clonal aneuploidies have been detected. Particular attention should be given to the use of low-oxygen environment in order to increase the proliferative capacity of hMSCs, since data on the effect of hypoxic culture conditions on genomic stability are few and contradictory. Furthermore, specific and reproducible epigenetic changes were acquired by hMSCs duringex vivoexpansion, which may be connected and trigger all the biological changes observed. In this review we address current issues on long-term culture of hMSCs with a 360-degree view, starting from the genomic profiles and back, looking for an epigenetic interpretation of their genetic stability.

Long-Term Culture and Transplantation of Spermatogonial Stem Cells from BALB/c Mice

2010 ◽  
Vol 191 (5) ◽  
pp. 372-381 ◽  
Author(s):  
Fujin Shen ◽  
Ci Zhang ◽  
Hongyun Zheng ◽  
Yunhe Xiong ◽  
Xi Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Long Term Culture

scholarly journals A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells

2014 ◽  
Vol 2 (11) ◽  
pp. 1683-1692 ◽  
Author(s):  
Cairnan R. E. Duffy ◽  
Rong Zhang ◽  
Siew-Eng How ◽  
Annamaria Lilienkampf ◽  
Guilhem Tourniaire ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Throughput ◽  
Polymer Substrates ◽  
Long Term Culture

Defined polymer substrates supported the growth of mesenchymal stem cells in long-term culture while maintaining their phenotype and lineage potential.

scholarly journals Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Daniela Hladik ◽  
Ines Höfig ◽  
Ursula Oestreicher ◽  
Johannes Beckers ◽  
Martina Matjanovski ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Genetic Instability ◽  
Dna Breaks ◽  
Long Term Culture
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