scholarly journals A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells

2014 ◽  
Vol 2 (11) ◽  
pp. 1683-1692 ◽  
Author(s):  
Cairnan R. E. Duffy ◽  
Rong Zhang ◽  
Siew-Eng How ◽  
Annamaria Lilienkampf ◽  
Guilhem Tourniaire ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Throughput ◽  
Polymer Substrates ◽  
Long Term Culture

Defined polymer substrates supported the growth of mesenchymal stem cells in long-term culture while maintaining their phenotype and lineage potential.

scholarly journals DNA Methylation Changes duringIn VitroPropagation of Human Mesenchymal Stem Cells: Implications for Their Genomic Stability?

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Angela Bentivegna ◽  
Mariarosaria Miloso ◽  
Gabriele Riva ◽  
Dana Foudah ◽  
Valentina Butta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ex Vivo ◽  
Genomic Stability ◽  
Great Promise ◽  
Human Mscs ◽  
Low Oxygen ◽  
Long Term Culture ◽  
Functional Changes

Mesenchymal stem cells (MSCs) hold great promise for the treatment of numerous diseases. A major problem for MSC therapeutic use is represented by the very low amount of MSCs which can be isolated from different tissues; thusex vivoexpansion is indispensable. Long-term culture, however, is associated with extensive morphological and functional changes of MSCs. In addition, the concern that they may accumulate stochastic mutations which lead the risk of malignant transformation still remains. Overall, the genome of human MSCs (hMSCs) appears to be apparently stable throughout culture, though transient clonal aneuploidies have been detected. Particular attention should be given to the use of low-oxygen environment in order to increase the proliferative capacity of hMSCs, since data on the effect of hypoxic culture conditions on genomic stability are few and contradictory. Furthermore, specific and reproducible epigenetic changes were acquired by hMSCs duringex vivoexpansion, which may be connected and trigger all the biological changes observed. In this review we address current issues on long-term culture of hMSCs with a 360-degree view, starting from the genomic profiles and back, looking for an epigenetic interpretation of their genetic stability.

scholarly journals Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Daniela Hladik ◽  
Ines Höfig ◽  
Ursula Oestreicher ◽  
Johannes Beckers ◽  
Martina Matjanovski ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Genetic Instability ◽  
Dna Breaks ◽  
Long Term Culture

Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro

2017 ◽  
Vol 19 (3) ◽  
pp. 159-170 ◽  
Author(s):  
Agnese Gugliandolo ◽  
Thangavelu Soundara Rajan ◽  
Domenico Scionti ◽  
Francesca Diomede ◽  
Placido Bramanti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture In Vitro ◽  
Long Term Culture ◽  
Oncogene Expression

Long-term culture optimization of human omentum fat-derived mesenchymal stem cells

2012 ◽  
Vol 36 (11) ◽  
pp. 1029-1036 ◽  
Author(s):  
Dhanasekaran Marappagounder ◽  
Indumathi Somasundaram ◽  
Rajkumar S. Janvikula ◽  
Sudarsanam Dorairaj
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Long Term Culture ◽  
Culture Optimization

scholarly journals Inhibition of autophagy alleviates the senescent state of rat mesenchymal stem cells during long-term culture

2014 ◽  
Vol 10 (6) ◽  
pp. 3003-3008 ◽  
Author(s):  
YONG ZHENG ◽  
CHENG-JUN HU ◽  
RU-HONG ZHUO ◽  
YUE-SHAN LEI ◽  
NA-NA HAN ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Long Term Culture

scholarly journals ID: 1054 Comparison between fresh and cryopreserved Human Amnion Mesenchymal Stem Cells (HAMCs) in terms of serial passaging, morphology and differentiation potential during long term culture

2017 ◽  
Vol 4 (S) ◽  
pp. 134
Author(s):  
Sandra Lisa Gumpil ◽  
Kamaruzaman Ampon ◽  
Helen Lasimbang ◽  
Simat Siti Fatimah ◽  
S.Vijay Kumar
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Cellular Therapy ◽  
Enzymatic Digestion ◽  
Positive Staining ◽  
Differentiation Potential ◽  
Human Amnion ◽  
Long Term Culture

The therapeutic efficiency of Human Amnion Mesenchymal stem cells (HAMCs) is significantly promising. However, its sustainability in long term sub-cultivation has yet to be identified, especially post-cryopreservation. There are concerns whether stem cells which are cryopreserved will be able to retain its functions and differentiation potential efficiently. The aim of this study is to determine the effects of cryopreservation on the serial passaging, morphology and differentiation potential of HAMCs culture post-cryopreservation compared to fresh HAMCs culture. HAMCs was isolated through serial enzymatic digestion of the amnion membrane of human term placenta after delivery. Fresh HAMCs cultures were sub-cultivated until passage 15 while cryopreserved HAMCs samples was preserved at passage 2 of the cell culture and revived after 3 months of cryopreservation period. Through microscopic observation, the cryopreserved HAMCs started to flatten and become larger in size, losing it fibroblastic feature as early as passage 6. The enlarged and flatten morphological features of the HAMCs indicate that the cells began to lose its proliferative activity at this point. In comparison, the HAMCs normally began to enlarge when reaching passage 15 in non-cryopreserved HAMCs cultures. Fresh HAMCs cultures were able to be sustained up to 15 passages while cryopreserved HAMCs were not able to survive pass 10 passages of cell culture. Furthermore, it was observed that both fresh and cryopreserved HAMCs were able to retain its differentiation potential through osteogenesis and adipogenesis medium induction. This was experimentally visualized through positive staining of the calcium composite and lipid droplet in the induced HAMCs culture using Von Kossa stain solution and Oil Red-O stain solution, respectively. Despite retaining it differentiation potential, cryopreserved HAMCs were only able to survive a few passages after being revived. The result suggests that fresh HAMCs is a more suitable candidate to be used in cellular therapy and various clinical application as it were able to retain all its function in long term culture.

scholarly journals Long-term culture in vitro impairs the immunosuppressive activity of mesenchymal stem cells on T cells

2012 ◽  
Vol 6 (5) ◽  
pp. 1183-1189 ◽  
Author(s):  
XUE-YI LI ◽  
JIN DING ◽  
ZHAO-HUI ZHENG ◽  
XIAO-YAN LI ◽  
ZHEN-BIAO WU ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cells ◽  
Mesenchymal Stem Cells ◽  
Immunosuppressive Activity ◽  
Culture In Vitro ◽  
Long Term Culture
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