Long-Term Culture and Transplantation of Spermatogonial Stem Cells from BALB/c Mice

2010 ◽  
Vol 191 (5) ◽  
pp. 372-381 ◽  
Author(s):  
Fujin Shen ◽  
Ci Zhang ◽  
Hongyun Zheng ◽  
Yunhe Xiong ◽  
Xi Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Long Term Culture

scholarly journals Expansion and long-term culture of human spermatogonial stem cells via the activation of SMAD3 and AKT pathways

2015 ◽  
Vol 240 (8) ◽  
pp. 1112-1122 ◽  
Author(s):  
Ying Guo ◽  
Linhong Liu ◽  
Min Sun ◽  
Yanan Hai ◽  
Zheng Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Long Term Culture

Genetic and epigenetic stability of human spermatogonial stem cells during long-term culture

2014 ◽  
Vol 102 (6) ◽  
pp. 1700-1707.e1 ◽  
Author(s):  
Bita Nickkholgh ◽  
S. Canan Mizrak ◽  
Saskia K.M. van Daalen ◽  
Cindy M. Korver ◽  
Hooman Sadri-Ardekani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Long Term Culture ◽  
Genetic And Epigenetic Stability

scholarly journals Culture of spermatogonial stem cells and use of surrogate sires as a breeding technology to propagate superior genetics in livestock production: A systematic review

2021 ◽  
pp. 3235-3248
Author(s):  
Wilkister Nakami ◽  
Ambrose Ng'eno Kipyegon ◽  
James Nguhiu-Mwangi ◽  
Christian Tiambo ◽  
Stephen Kemp
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Data Extraction ◽  
Transgenic Animals ◽  
Spermatogonial Stem Cells ◽  
Published Data ◽  
Long Term Culture ◽  
Future Possibility

Background and Aim: Spermatogonial stem cells (SSCs) have previously been isolated from animals' testes, cultured in vitro, and successfully transplanted into compatible recipients. The SSC unique characteristic has potential for exploitation as a reproductive tool and this can be achieved through SSC intratesticular transplantation to surrogate sires. Here, we aimed at comprehensively analyzing published data on in vitro maintenance of SSC isolated from the testes of livestock animals and their applications. Materials and Methods: The literature search was performed in PubMed, Science Direct, and Google Scholar electronic databases. Data screening was conducted using Rayyan Intelligent Systematic Review software (https://www.rayyan.ai/). Duplicate papers were excluded from the study. Abstracts were read and relevant full papers were reviewed for data extraction. Results: From a total of 4786 full papers screened, data were extracted from 93 relevant papers. Of these, eight papers reported on long-term culture conditions (>1 month) for SSC in different livestock species, 22 papers on short-term cultures (5-15 days), 10 papers on transfection protocols, 18 papers on transplantation using different methods of preparation of livestock recipients, and five papers on donor-derived spermatogenesis. Conclusion: Optimization of SSC long-term culture systems has renewed the possibilities of utilization of these cells in gene-editing technologies to develop transgenic animals. Further, the development of genetically deficient recipients in the endogenous germline layer lends to a future possibility for the utilization of germ cell transplantation in livestock systems.

An Alternative Long-Term Culture System for Highly-Pure Mouse Spermatogonial Stem Cells

2015 ◽  
Vol 230 (6) ◽  
pp. 1365-1375 ◽  
Author(s):  
Bao-Rong He ◽  
Fan Lu ◽  
Lingling Zhang ◽  
Ding-Jun Hao ◽  
Hao Yang
Keyword(s):  
Stem Cells ◽  
Culture System ◽  
Spermatogonial Stem Cells ◽  
Long Term Culture
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