Fibrillar films obtained from sodium soap fibers and polyelectrolyte multilayers

2011 ◽  
Vol 98A (2) ◽  
pp. 287-295 ◽  
Author(s):  
Scott A. Zawko ◽  
Christine E. Schmidt
Keyword(s):  
Polyelectrolyte Multilayers ◽  
Sodium Soap

scholarly journals Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ivan Ding ◽  
Amy M. Peterson
Keyword(s):  
Cell Culture ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Half Life ◽  
Cell Types ◽  
Polyelectrolyte Multilayers ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fibroblast Growth ◽  
Cell Culture Study

AbstractGrowth factor-eluting polymer systems have been widely reported to improve cell and tissue outcomes; however, measurements of actual growth factor concentration in cell culture conditions are limited. The problem is compounded by a lack of knowledge of growth factor half-lives, which impedes efforts to determine real-time growth factor concentrations. In this work, the half-life of basic fibroblast growth factor (FGF2) was determined using enzyme linked immunosorbent assay (ELISA). FGF2 release from polyelectrolyte multilayers (PEMs) was measured and the data was fit to a simple degradation model, allowing for the determination of FGF2 concentrations between 2 and 4 days of culture time. After the first hour, the FGF2 concentration for PEMs assembled at pH = 4 ranged from 2.67 ng/mL to 5.76 ng/mL, while for PEMs assembled at pH = 5, the concentration ranged from 0.62 ng/mL to 2.12 ng/mL. CRL-2352 fibroblasts were cultured on PEMs assembled at pH = 4 and pH = 5. After 2 days, the FGF2-eluting PEM conditions showed improved cell count and spreading. After 4 days, only the pH = 4 assembly condition had higher cells counts, while the PEM assembled at pH = 5 and PEM with no FGF2 showed increased spreading. Overall, the half-life model and cell culture study provide optimal concentration ranges for fibroblast proliferation and a framework for understanding how temporal FGF2 concentration may affect other cell types.

scholarly journals Electrical Field-Assisted Gene Delivery from Polyelectrolyte Multilayers

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 133
Author(s):  
Yu-Che Cheng ◽  
Shu-Lin Guo ◽  
Kun-Da Chung ◽  
Wei-Wen Hu
Keyword(s):  
Gene Delivery ◽  
Electric Field ◽  
Treatment Time ◽  
Transfection Efficiency ◽  
Polyelectrolyte Multilayers ◽  
Aqueous Environment ◽  
Layer By Layer ◽  
Electrical Field ◽  
Lbl Assembly ◽  
Electric Field Treatment

To sustain gene delivery and elongate transgene expression, plasmid DNA and cationic nonviral vectors can be deposited through layer-by-layer (LbL) assembly to form polyelectrolyte multilayers (PEMs). Although these macromolecules can be released for transfection purposes, their entanglement only allows partial delivery. Therefore, how to efficiently deliver immobilized genes from PEMs remains a challenge. In this study, we attempt to facilitate their delivery through the pretreatment of the external electrical field. Multilayers of polyethylenimine (PEI) and DNA were deposited onto conductive polypyrrole (PPy), which were placed in an aqueous environment to examine their release after electric field pretreatment. Only the electric field perpendicular to the substrate with constant voltage efficiently promoted the release of PEI and DNA from PEMs, and the higher potential resulted in the more releases which were enhanced with treatment time. The roughness of PEMs also increased after electric field treatment because the electrical field not only caused electrophoresis of polyelectrolytes and but also allowed electrochemical reaction on the PPy electrode. Finally, the released DNA and PEI were used for transfection. Polyplexes were successfully formed after electric field treatment, and the transfection efficiency was also improved, suggesting that this electric field pretreatment effectively assists gene delivery from PEMs and should be beneficial to regenerative medicine application.

Novel Application of Polyelectrolyte Multilayers as Nanoscopic Closures with Hermetic Sealing

2012 ◽  
Vol 4 (3) ◽  
pp. 1620-1628 ◽  
Author(s):  
Stephanie A. Marcott ◽  
Sena Ada ◽  
Phillip Gibson ◽  
Terri A. Camesano ◽  
R. Nagarajan
Keyword(s):  
Polyelectrolyte Multilayers ◽  
Hermetic Sealing

Accelerated Exchange in Polyelectrolyte Multilayers by “Catalytic” Polyvalent Ion Pairing

Langmuir ◽  
2005 ◽  
Vol 21 (18) ◽  
pp. 8081-8084 ◽  
Author(s):  
Houssam W. Jomaa ◽  
Joseph B. Schlenoff
Keyword(s):  
Ion Pairing ◽  
Polyelectrolyte Multilayers

scholarly journals Effect of substrate on the adsorption of polyelectrolyte multilayers: Study by optical fixed-angle reflectometry and AFM

2007 ◽  
Vol 305 (1-3) ◽  
pp. 105-111 ◽  
Author(s):  
C.C. Buron ◽  
C. Filiâtre ◽  
F. Membrey ◽  
C. Bainier ◽  
D. Charraut ◽  
...  
Keyword(s):  
Polyelectrolyte Multilayers ◽  
Fixed Angle
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