scholarly journals Electrical Field-Assisted Gene Delivery from Polyelectrolyte Multilayers

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 133
Author(s):  
Yu-Che Cheng ◽  
Shu-Lin Guo ◽  
Kun-Da Chung ◽  
Wei-Wen Hu
Keyword(s):  
Gene Delivery ◽  
Electric Field ◽  
Treatment Time ◽  
Transfection Efficiency ◽  
Polyelectrolyte Multilayers ◽  
Aqueous Environment ◽  
Layer By Layer ◽  
Electrical Field ◽  
Lbl Assembly ◽  
Electric Field Treatment

To sustain gene delivery and elongate transgene expression, plasmid DNA and cationic nonviral vectors can be deposited through layer-by-layer (LbL) assembly to form polyelectrolyte multilayers (PEMs). Although these macromolecules can be released for transfection purposes, their entanglement only allows partial delivery. Therefore, how to efficiently deliver immobilized genes from PEMs remains a challenge. In this study, we attempt to facilitate their delivery through the pretreatment of the external electrical field. Multilayers of polyethylenimine (PEI) and DNA were deposited onto conductive polypyrrole (PPy), which were placed in an aqueous environment to examine their release after electric field pretreatment. Only the electric field perpendicular to the substrate with constant voltage efficiently promoted the release of PEI and DNA from PEMs, and the higher potential resulted in the more releases which were enhanced with treatment time. The roughness of PEMs also increased after electric field treatment because the electrical field not only caused electrophoresis of polyelectrolytes and but also allowed electrochemical reaction on the PPy electrode. Finally, the released DNA and PEI were used for transfection. Polyplexes were successfully formed after electric field treatment, and the transfection efficiency was also improved, suggesting that this electric field pretreatment effectively assists gene delivery from PEMs and should be beneficial to regenerative medicine application.

Boosting transfection efficiency: A systematic study using layer-by-layer based gene delivery platform

2021 ◽  
pp. 112161
Author(s):  
Yana V. Tarakanchikova ◽  
Dmitrii S. Linnik ◽  
Tatiana Mashel ◽  
Albert R. Muslimov ◽  
Sergey Pavlov ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Systematic Study ◽  
Transfection Efficiency ◽  
Layer By Layer ◽  
Delivery Platform

Electric-field induced layer-by-layer assembly technique with single component for construction of conjugated polymer films

RSC Advances ◽  
2015 ◽  
Vol 5 (72) ◽  
pp. 58499-58503 ◽  
Author(s):  
Shiwei Wang ◽  
Zhuo Chen ◽  
Ahmad Umar ◽  
Yao Wang ◽  
Peng-gang Yin
Keyword(s):  
Electric Field ◽  
Conjugated Polymer ◽  
Multilayer Films ◽  
Single Component ◽  
Layer By Layer ◽  
Lbl Assembly ◽  
Layer By Layer Assembly ◽  
Universal Approach ◽  
Assembly Technique ◽  
Layer Assembly

Single component was used to construct conjugated polymer multilayer films by electric-field induced layer-by-layer assembly technique, which provides a universal approach for CPs and broadens the applicable scope of LBL assembly technique.

Changes in Layer Properties and Physical Properties of Papers from Old Corrugated Container Fibres with PDADMAC/HEC Polyelectrolyte Multilayers

2012 ◽  
Vol 496 ◽  
pp. 67-70 ◽  
Author(s):  
Zhi Yuan Yang ◽  
Hai Zhen Shi ◽  
Yan Jun Tang
Keyword(s):  
Adsorbed Layer ◽  
Polyelectrolyte Multilayers ◽  
Raw Material ◽  
Hydroxyethyl Cellulose ◽  
Layer By Layer ◽  
Filtration Time ◽  
Physical Strength ◽  
Tensile Index ◽  
Lbl Assembly ◽  
Fibre Suspension

In this paper, Old Corrugated Container (OCC) was used as the raw material for making paper. The layer-by-layer (LBL) assembly process of creating highly structured thin films was adopted to modify the surface of OCC fibres. Poly dimethyldiallylammonium chloride (PDADMAC) and hydroxyethyl cellulose (HEC) have been used to build up polyelectrolyte multilayers on OCC fibres under a certain condition. Zeta potential analyzer was used to monitor the dynamic adsorption of PDADMAC and HEC with different amount on OCC fibers. Strength measurements of the sheets including tensile index and bursting index were also determined, showing a significant improvement in physical strength with the increase of adsorbed layer number. Compared with not being processed sheets, tensile index and bursting index of sheets from treated fibre with polyelectrolyte multilayers were raised by 36.65% and 49.50%, respectively. Furthermore, filtration time analysis of OCC fibre suspension showed that the built up of PDADMAC and HEC on OCC fibres could improve the fiber-fiber joint strength of joints from polymer-treated fibers, which made the filtration time decline from 30 s to 10.6 s. In addition, SEM was introduced to observe the morphology of the PDADMAC/HEC multilayers. The images revealed that the surface of OCC fibres structured a film like plastic, which could be ascribed to the adsorption layer of polyelectrolyte.

Electrohydrodynamic Direct-Write Micro/Nano Pattern with the Restriction of Inducing Tip

2015 ◽  
Vol 748 ◽  
pp. 135-139 ◽  
Author(s):  
Zhao Jie Yu ◽  
Jia Xin Jiang ◽  
Ling Ling Sun ◽  
Gao Feng Zheng ◽  
Dao Heng Sun
Keyword(s):  
Electric Field ◽  
Large Scale ◽  
Layer By Layer ◽  
Direct Write ◽  
Three Dimension ◽  
Fabrication Method ◽  
Electrical Field ◽  
Motion Stage ◽  
The Stability ◽  
Focused Electric Field

As a novel and simple non-contact fabrication method, electrohydrodynamic direct-write (EDW) has been regarded as one of the most potential technologies for large scale manufacturing of flexible and organic electronics. An inducing tip was introduced into the EDW apparatus. The enhanced electrical field between spinneret and inducing tip provided an excellent way to restrict the motion of charged jet, by which the stabilization of EDW jet can be promoted and the disturbances can also be overcome. Charged jet is deposited layer by layer to construct three dimension structure between spinneret and collector. The three dimension structure grows directly to the spinneret by the guidance and restriction of focused electric field. With the help of X-Y motion stage, the motion track of collector can be controlled by the host computer to fabricate designed pattern. The inducing tip provides a good way to increase the stability and deposition accurate of EDW charged jet.

Electric-Field-Assisted Layer-by-Layer Assembly of Weakly Charged Polyelectrolyte Multilayers

2011 ◽  
Vol 44 (8) ◽  
pp. 2866-2872 ◽  
Author(s):  
Young Hoon Ko ◽  
Young Hun Kim ◽  
Juhyun Park ◽  
Ki Tae Nam ◽  
Jong Hyeok Park ◽  
...  
Keyword(s):  
Electric Field ◽  
Polyelectrolyte Multilayers ◽  
Layer By Layer ◽  
Layer By Layer Assembly ◽  
Layer Assembly

Enhancement of Pulp Strength by Forming Polyelectrolyte Multilayers on APMP Fibers

2014 ◽  
Vol 496-500 ◽  
pp. 171-174
Author(s):  
Ge Tian ◽  
Yun Yun Sun ◽  
Fan Gong Kong ◽  
Shou Juan Wang ◽  
Jia Chuan Chen
Keyword(s):  
Treatment Time ◽  
Fiber Surface ◽  
Strength Properties ◽  
Polyelectrolyte Multilayers ◽  
Layer By Layer ◽  
Physical Strength ◽  
Alkaline Peroxide ◽  
Treatment Conditions ◽  
Layer Deposition ◽  
Pulp Strength

The layer-by-layer deposition technique was adopted in this paper to improve the physical strength properties of alkaline peroxide mechanical pulp (APMP). The cationic starch (CS), anionic polyacryamide (APAM) and cationic polyacryamide (CPAM) were chosen and used to build-up polyelectrolyte multilayers on surface of APMP fibers. The improvements of physical strength of pulp through adsorption of different polyelectrolyte onto fibers were discussed and compared. The results showed that when the APMP fibers were treated with CPAM, the breaking length and burst index were 53% and 83% respectively, higher than that of untreated pulp. The optimal treatment conditions are 60 mgCPAM/g pulp, 1.5% pulp concentration and 9min treatment time. The pulp deposited by CPAM-APAM polyelectrolyte multilayer gave a highest physical strength compared with pulp with other multilayer deposition such as CS-APAM. At the whole beating degree range investigated in this paper, the CPAM-APAM deposition on fiber surface can improve the physical strength properties significantly, especially when the beating degree is at 40oSR. In addition, the improvement of physical strength can be remained even after pulp refining.

Stepwise Development of Biomimetic Chimeric Peptides for Gene Delivery

2020 ◽  
Vol 27 (8) ◽  
pp. 698-710
Author(s):  
Roya Cheraghi ◽  
Mahboobeh Nazari ◽  
Mohsen Alipour ◽  
Saman Hosseinkhani
Keyword(s):  
Gene Delivery ◽  
Targeted Delivery ◽  
Viral Vectors ◽  
Large Scale ◽  
Transfection Efficiency ◽  
Cationic Lipids ◽  
Target Cells ◽  
Scale Production ◽  
Stepwise Development ◽  
Chimeric Peptides

Gene-based therapy largely relies on the vector type that allows a selective and efficient transfection into the target cells with maximum efficacy and minimal toxicity. Although, genes delivered utilizing modified viruses transfect efficiently and precisely, these vectors can cause severe immunological responses and are potentially carcinogenic. A promising method of overcoming this limitation is the use of non-viral vectors, including cationic lipids, polymers, dendrimers, and peptides, which offer potential routes for compacting DNA for targeted delivery. Although non-viral vectors exhibit reduced transfection efficiency compared to their viral counterpart, their superior biocompatibility, non-immunogenicity and potential for large-scale production make them increasingly attractive for modern therapy. There has been a great deal of interest in the development of biomimetic chimeric peptides. Biomimetic chimeric peptides contain different motifs for gene translocation into the nucleus of the desired cells. They have motifs for gene targeting into the desired cell, condense DNA into nanosize particles, translocate the gene into the nucleus and enhance the release of the particle into the cytoplasm. These carriers were developed in recent years. This review highlights the stepwise development of the biomimetic chimeric peptides currently being used in gene delivery.

Sign in / Sign up

Export Citation Format

Share Document