Surface immobilization of poly(ethyleneimine) and plasmid DNA on electrospun poly(L-lactic acid) fibrous mats using a layer-by-layer approach for gene delivery

2009 ◽  
Vol 88A (2) ◽  
pp. 281-287 ◽  
Author(s):  
Shinji Sakai ◽  
Yusuke Yamada ◽  
Tetsu Yamaguchi ◽  
Tomasz Ciach ◽  
Koei Kawakami
Keyword(s):  
Lactic Acid ◽  
Gene Delivery ◽  
Plasmid Dna ◽  
Layer By Layer ◽  
Surface Immobilization

scholarly journals Surface Immobilization of Plasmid DNA with a Cell-Responsive Tether for Substrate-Mediated Gene Delivery

Langmuir ◽  
2011 ◽  
Vol 27 (6) ◽  
pp. 2739-2746 ◽  
Author(s):  
Kory M. Blocker ◽  
Kristi L. Kiick ◽  
Millicent O. Sullivan
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
Surface Immobilization ◽  
A Cell

Adsorption of Plasmid DNA ontoN,N‘- (Dimethylamino)ethyl-methacrylate Graft-Polymerized Poly-l-lactic Acid Film Surface for Promotion of in-Situ Gene Delivery

2007 ◽  
Vol 8 (6) ◽  
pp. 1951-1957 ◽  
Author(s):  
Tingting Jiang ◽  
Chang ◽  
Chunming Wang ◽  
Zhi Ding ◽  
Jiangning Chen ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Gene Delivery ◽  
Plasmid Dna ◽  
Film Surface ◽  
Ethyl Methacrylate

Boosting transfection efficiency: A systematic study using layer-by-layer based gene delivery platform

2021 ◽  
pp. 112161
Author(s):  
Yana V. Tarakanchikova ◽  
Dmitrii S. Linnik ◽  
Tatiana Mashel ◽  
Albert R. Muslimov ◽  
Sergey Pavlov ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Systematic Study ◽  
Transfection Efficiency ◽  
Layer By Layer ◽  
Delivery Platform

scholarly journals Electrical Field-Assisted Gene Delivery from Polyelectrolyte Multilayers

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 133
Author(s):  
Yu-Che Cheng ◽  
Shu-Lin Guo ◽  
Kun-Da Chung ◽  
Wei-Wen Hu
Keyword(s):  
Gene Delivery ◽  
Electric Field ◽  
Treatment Time ◽  
Transfection Efficiency ◽  
Polyelectrolyte Multilayers ◽  
Aqueous Environment ◽  
Layer By Layer ◽  
Electrical Field ◽  
Lbl Assembly ◽  
Electric Field Treatment

To sustain gene delivery and elongate transgene expression, plasmid DNA and cationic nonviral vectors can be deposited through layer-by-layer (LbL) assembly to form polyelectrolyte multilayers (PEMs). Although these macromolecules can be released for transfection purposes, their entanglement only allows partial delivery. Therefore, how to efficiently deliver immobilized genes from PEMs remains a challenge. In this study, we attempt to facilitate their delivery through the pretreatment of the external electrical field. Multilayers of polyethylenimine (PEI) and DNA were deposited onto conductive polypyrrole (PPy), which were placed in an aqueous environment to examine their release after electric field pretreatment. Only the electric field perpendicular to the substrate with constant voltage efficiently promoted the release of PEI and DNA from PEMs, and the higher potential resulted in the more releases which were enhanced with treatment time. The roughness of PEMs also increased after electric field treatment because the electrical field not only caused electrophoresis of polyelectrolytes and but also allowed electrochemical reaction on the PPy electrode. Finally, the released DNA and PEI were used for transfection. Polyplexes were successfully formed after electric field treatment, and the transfection efficiency was also improved, suggesting that this electric field pretreatment effectively assists gene delivery from PEMs and should be beneficial to regenerative medicine application.

Layer-by-Layer Biomimetic Microgels for 3D Cell Culture and Nonviral Gene Delivery

2021 ◽  
Author(s):  
Bruna G. Carvalho ◽  
Franciele F. Vit ◽  
Hernandes F. Carvalho ◽  
Sang W. Han ◽  
Lucimara G. de la Torre
Keyword(s):  
Cell Culture ◽  
Gene Delivery ◽  
3D Cell Culture ◽  
Nonviral Gene Delivery ◽  
Layer By Layer

Plasmid DNA microgels for cancer treatment through combination of chemotherapy and gene delivery

2014 ◽  
Vol 185 ◽  
pp. S35
Author(s):  
Diana Barata Costa ◽  
Artur Monteiro Valente ◽  
João Sampaio Queiroz
Keyword(s):  
Gene Delivery ◽  
Cancer Treatment ◽  
Plasmid Dna

Alkylimidazolium End-Modified Poly(ethylene glycol) To Form the Mono-ion Complex with Plasmid DNA for in Vivo Gene Delivery

2014 ◽  
Vol 15 (3) ◽  
pp. 997-1001 ◽  
Author(s):  
Shoichiro Asayama ◽  
Atsushi Nohara ◽  
Yoichi Negishi ◽  
Hiroyoshi Kawakami
Keyword(s):  
Gene Delivery ◽  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Poly Ethylene Glycol ◽  
In Vivo Gene Delivery ◽  
Poly Ethylene
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