scholarly journals Surface Immobilization of Plasmid DNA with a Cell-Responsive Tether for Substrate-Mediated Gene Delivery

Langmuir ◽  
2011 ◽  
Vol 27 (6) ◽  
pp. 2739-2746 ◽  
Author(s):  
Kory M. Blocker ◽  
Kristi L. Kiick ◽  
Millicent O. Sullivan
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
Surface Immobilization ◽  
A Cell

scholarly journals Magnetic Nanoparticles Mediated-Gene Delivery for Simpler and More Effective Transformation of Pichia pastoris

2021 ◽  
Author(s):  
Seyda Yildiz ◽  
Kubra Solak ◽  
Melek Acar ◽  
Ahmet Mavi ◽  
Yagmur Unver
Keyword(s):  
Magnetic Nanoparticles ◽  
Pichia Pastoris ◽  
Gene Delivery ◽  
Delivery Method ◽  
Exogenous Dna ◽  
Novel Gene ◽  
A Cell

The introduction of exogenous DNA into a cell can be used to produce large quantities of protein. Here, we describe a novel gene delivery method into Pichia pastoris based on...

scholarly journals A Cell-Penetrating Peptide with a Guanidinylethyl Amine Structure Directed to Gene Delivery

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Makoto Oba ◽  
Takuma Kato ◽  
Kaori Furukawa ◽  
Masakazu Tanaka
Keyword(s):  
Gene Delivery ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Amine Structure ◽  
A Cell

Plasmid DNA microgels for cancer treatment through combination of chemotherapy and gene delivery

2014 ◽  
Vol 185 ◽  
pp. S35
Author(s):  
Diana Barata Costa ◽  
Artur Monteiro Valente ◽  
João Sampaio Queiroz
Keyword(s):  
Gene Delivery ◽  
Cancer Treatment ◽  
Plasmid Dna

scholarly journals Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery

2019 ◽  
Vol 244 (2) ◽  
pp. 100-113 ◽  
Author(s):  
Amy Mantz ◽  
Angela K Pannier
Keyword(s):  
Gene Delivery ◽  
Intracellular Trafficking ◽  
Cellular Response ◽  
Adhesion Formation ◽  
Nonviral Gene Delivery ◽  
Material Interface ◽  
Cytoskeletal Remodeling ◽  
Cell Material ◽  
Focal Adhesion Formation ◽  
A Cell

Gene delivery is the transfer of exogenous genetic material into somatic cells to modify their gene expression, with applications including tissue engineering, regenerative medicine, sensors and diagnostics, and gene therapy. Viral vectors are considered the most effective system to deliver nucleic acids, yet safety concerns and many other disadvantages have resulted in investigations into an alternative option, i.e. nonviral gene delivery. Chemical nonviral gene delivery is typically accomplished by electrostatically complexing cationic lipids or polymers with negatively charged nucleic acids. Unfortunately, nonviral gene delivery suffers from low efficiency due to barriers that impede transfection success, including intracellular processes such as internalization, endosomal escape, cytosolic trafficking, and nuclear entry. Efforts to improve nonviral gene delivery have focused on modifying nonviral vectors, yet a novel solution that may prove more effective than vector modifications is stimulating or “priming” cells before transfection to modulate and mitigate the cellular response to nonviral gene delivery. In applications where a cell-material interface exists, cell priming can come from cues from the substrate, through chemical modifications such as the addition of natural coatings, ligands, or functional side groups, and/or physical modifications such as topography or stiffness, to mimic extracellular matrix cues and modulate cellular behaviors that influence transfection efficiency. This review summarizes how biomaterial substrate modifications can prime the cellular response to nonviral gene delivery (e.g. integrin binding and focal adhesion formation, cytoskeletal remodeling, endocytic mechanisms, intracellular trafficking) to aid in improving gene delivery for future therapeutic applications. Impact statement This review summarizes how biomaterial substrate modifications (e.g. chemical modifications like natural coatings, ligands, or functional side groups, and/or physical modifications such as topography or stiffness) can prime the cellular response to nonviral gene delivery (e.g. affecting integrin binding and focal adhesion formation, cytoskeletal remodeling, endocytic mechanisms, and intracellular trafficking), to aid in improving gene delivery for applications where a cell-material interface might exist (e.g. tissue engineering scaffolds, medical implants and devices, sensors and diagnostics, wound dressings).

Alkylimidazolium End-Modified Poly(ethylene glycol) To Form the Mono-ion Complex with Plasmid DNA for in Vivo Gene Delivery

2014 ◽  
Vol 15 (3) ◽  
pp. 997-1001 ◽  
Author(s):  
Shoichiro Asayama ◽  
Atsushi Nohara ◽  
Yoichi Negishi ◽  
Hiroyoshi Kawakami
Keyword(s):  
Gene Delivery ◽  
Ethylene Glycol ◽  
Plasmid Dna ◽  
Poly Ethylene Glycol ◽  
In Vivo Gene Delivery ◽  
Poly Ethylene

Plasmid DNA Mono-Ion Complex Stabilized by Hydrogen Bond for In Vivo Diffusive Gene Delivery

2015 ◽  
Vol 16 (4) ◽  
pp. 1226-1231 ◽  
Author(s):  
Shoichiro Asayama ◽  
Atsushi Nohara ◽  
Yoichi Negishi ◽  
Hiroyoshi Kawakami
Keyword(s):  
Hydrogen Bond ◽  
Gene Delivery ◽  
Plasmid Dna
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