scholarly journals Pyruvate kinase type M2 is upregulated in colorectal cancer and promotes proliferation and migration of colon cancer cells

IUBMB Life ◽  
2012 ◽  
Vol 64 (9) ◽  
pp. 775-782 ◽  
Author(s):  
Cheng-Fu Zhou ◽  
Xue-Bing Li ◽  
Heng Sun ◽  
Bo Zhang ◽  
Yu-Song Han ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Pyruvate Kinase ◽  
Colon Cancer Cells ◽  
And Migration ◽  
Proliferation And Migration

Kopsanone inhibits proliferation and migration of invasive colon cancer cells

2021 ◽  
Author(s):  
Daniella Paiva Bonfim ◽  
Celso Vataru Nakamura ◽  
João Xavier Araújo Júnior ◽  
Greisiele Lorena Pessini ◽  
Paulo Emílio Correa Leite ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals LncRNA DSCAM-AS1 Promotes Colon Cancer Cells Proliferation and Migration via Regulating the miR-204/SOX4 Axis

2020 ◽  
Vol Volume 12 ◽  
pp. 4347-4356
Author(s):  
Canrong Lu ◽  
Tianyu Xie ◽  
Xin Guo ◽  
Di Wu ◽  
Shuo Li ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals MicroRNA 375 regulates proliferation and migration of colon cancer cells by suppressing the CTGF-EGFR signaling pathway

2017 ◽  
Vol 141 (8) ◽  
pp. 1614-1629 ◽  
Author(s):  
Khondoker Jahengir Alam ◽  
Ji-Su Mo ◽  
Seol-Hee Han ◽  
Won-Cheol Park ◽  
Hun-Soo Kim ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Colon Cancer Cells ◽  
Egfr Signaling ◽  
Egfr Signaling Pathway ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals 864 The mesenchymal stromal compartment in colorectal cancer greatly alters the innate tumour immune microenvironment both in 2D and 3D culture systems

2020 ◽  
Vol 8 (Suppl 3) ◽  
pp. A917-A917
Author(s):  
Niamh Leonard ◽  
Oliver Treacy ◽  
Hannah Egan ◽  
Grace O’Malley ◽  
Elena Tomas Bort ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Bone Marrow ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Tumour Cell ◽  
Culture System ◽  
Antigen Processing ◽  
Colon Cancer Cells ◽  
Immune Microenvironment ◽  
And Migration

BackgroundColorectal cancer is the fourth most common occurring cancer and despite new treatment options it remains the third leading cause of cancer related deaths worldwide.1 Of the four Consensus Molecular Subtypes (CMS), the mesenchymal stromal rich CMS4 tumours are shown to have the worst disease free progression survival. However the role mesenchymal stromal cells (MSC) play in the tumour immune microenvironment has yet to be fully elucidated. Understanding the complex communication in this stromal cell rich multicellular environment is challenging but may reveal novel targets for the treatment of colorectal cancer patients.MethodsTumour cell secretome (TCS) was generated from colon cancer cells with/without the addition of TNF-a, an inflammatory stimulus using both human and mouse cell lines. MSCs were then conditioned with the TCS and inflammatory TCS and changes in surface and secreted immunomodulatory molecules were assessed using RNA-seq, flow cytometry and ELISA analysis. Macrophage antigen processing and migration following co-culture with the TCS conditioned MSCs was observed using DQ-ova and transwell experiments. A Gelatin Methacryloyl hydrogel, 3D triple culture systems was established to study the role of MSC in the colon tumour immune microenvironment. HCT116 colon cancer cell line with THP1 monocytic cell line and primary bone marrow derived MSCs were embedded in the hydrogel and incubated for 10days, changing the media on Day 8 with/without the addition of TNF-a. Cell proliferation viability and protein secretion were assessed from the 3D CRC system.ResultsBioplex analysis revealed secretion of potent chemokines and cytokines from the cancer cells. This inflammatory TSC resulted in increased expression of cell surface MSC immunomodulatory markers PD-L1 and CD47 and a variety of secreted molecules. These conditioned MSCs reduced macrophage-mediated antigen processing and increase monocyte migration. A triple culture 3D model of CRC was successfully developed, and while the addition of MSC to the system did not alter spheroid size they increased the release of potent chemokines(CCL2, CXCL12), cytokines (IL-6, IL8) and growth factors (GM-CSF) from the culture system.ConclusionsThe inflammatory tumour cell secretome can alter MSC surface expression and secretion of a variety of immunomodulatory makers. These tumour conditioned MSCs can alter innate immune cell antigen processing and migration. When MSCs are combined in 3D with monocytes and colon cancer cells the MSC significantly alter the secretion of immune modulating and tumour promoting factors from the culture system. Targeting MSC immune suppression in the colon tumour microenvironment could be a novel therapeutic target.Ethics ApprovalHuman MSC (hMSC) were isolated from the bone marrow of three healthy volunteers at Galway University Hospital under an ethically approved protocol (NUIG Research Ethics Committee, Ref: 08/May/14) according to a standardized procedure.ReferenceRawla P, Sunkara T, Barsouk A. Epidemiology of colorectal cancer: incidence, mortality, survival, and risk factors. Prz Gastroenterol 2019;14(2):89-103.

scholarly journals FYN is required for ARHGEF16 to promote proliferation and migration in colon cancer cells

2020 ◽  
Vol 11 (8) ◽  
Author(s):  
Bei Yu ◽  
Linlin Xu ◽  
Limin Chen ◽  
Yao Wang ◽  
Hongying Jiang ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
And Migration ◽  
Proliferation And Migration
Sign in / Sign up

Export Citation Format

Share Document