Cytolytic activity of macrophages isolated from primary murine sarcoma virus (MSV)-induced tumors

1979 ◽  
Vol 24 (2) ◽  
pp. 151-160 ◽  
Author(s):  
Tadayoshi Taniyama ◽  
Howard T. Holden
Keyword(s):  
Cytolytic Activity ◽  
Induced Tumors ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma

scholarly journals Quantitation and clonal isolation of cytolytic T lymphocyte precursors selectively infiltrating murine sarcoma virus-induced tumors.

1981 ◽  
Vol 154 (2) ◽  
pp. 362-373 ◽  
Author(s):  
K T Brunner ◽  
H R MacDonald ◽  
J C Cerottini
Keyword(s):  
Leukemia Virus ◽  
Cytolytic Activity ◽  
Target Cells ◽  
Blood Leukocytes ◽  
Induced Tumors ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Leukocyte Populations ◽  
Murine Sarcoma ◽  
Selective Accumulation

A limiting dilution mixed leukocyte-tumor cell microculture system was used to quantitate cytolytic T lymphocytes and their precursors (CTL-P), which infiltrate tumors induced by injection of Moloney sarcoma-leukemia virus (MSV-MoLV) complex into C57BL/6 mice. Leukocyte populations obtained from tumors collected on day 10 after virus injection were found to contain significantly higher frequencies of operationally defined (tumor-specific) CTL-P than either peripheral blood leukocytes (PBL) or spleen cells from the same animals. When these frequencies were normalized according to the content of Lyt-2+ T cells in each tissue, average CTL-P frequencies were found to be 1/9 in tumor-infiltrating cells vs. 1/41 in PBL. These results directly demonstrate selective accumulation of CTL-P in the tumor mass. A number of clonal isolates obtained from tumor-infiltrating leukocyte populations were expanded and studied for cytolytic activity and specificity. Of 11 isolates, 10 were found to have high cytolytic activity, leading to 50% lysis of the syngeneic MoLV-derived tumor target cells in 3.5 h at lymphocyte:target cell ratios ranging from 0.5:1 to 3.2:1. Furthermore, five randomly selected clones showed H-2 restriction by their selective lytic activity against MoLV-derived syngeneic MBL-2 target cells and their lack of activity against either MoLV-derived allogeneic (LSTRA) tumor cells or against syngeneic (EL4) or allogeneic (P815) target cells unrelated to MoLV.

Enhancement and Suppression of Murine Sarcoma Virus Induced Tumors by Polyriboinosinic Polyribocytidylic Acid

1972 ◽  
Vol 139 (1) ◽  
pp. 279-285 ◽  
Author(s):  
A. F. Gazdar ◽  
A. J. Weinstein ◽  
H. L. Sims ◽  
A. D. Steinberg
Keyword(s):  
Induced Tumors ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Murine Sarcoma

A Report Of Morphologically Aberrant Virus Particles in Cells Infected With MSV (FelLV) Virus

1973 ◽  
Vol 31 ◽  
pp. 584-585
Author(s):  
R. A. Al-Adhami ◽  
A. L. Chapman
Keyword(s):  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Virus Particles ◽  
Induced Tumors ◽  
Embryo Cells ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Mouse Cells ◽  
Type C ◽  
Murine Sarcoma

Fujinaga et al reported MSV induced rat and hamster osteosarcoma which showed an occassional unusual bud in the rat induced tumors. Savage and Hackett and Hackett and Sylvester reported abnormal type C virus in UCLB cells derived from Balb/3t3 cells infected and transformed with MLV. They wer unable to demonstrate sarcoma virus activity. Fischinger and O‘Connor reported the infection of cat embryo cells by a centrifugally induced aggregate of murine sarcoma virus and feline leukemia virus designated as MSV(FelLV). This virus gave rise to a defective, focus forming virus which propagated in cat cells but not in mouse cells.In the present study the morphoiogy of the MSV(FelLV) virus obtained from Dr. Fischinger and maintained in our laboratory since 1970 will be reported. Feline embryo fibroblasts (established in our lab.) and Crandall feline kidney cells (Cutter-Haver-Lockhart, Shawnee, Kansas) were used in this study.

Histopathologic and Ultrastructural Study of Tumors Induced by Murine Sarcoma Virus (MSV).

1975 ◽  
Vol 61 (2) ◽  
pp. 129-150 ◽  
Author(s):  
Natale Pennelli ◽  
Luigi Chieco-Bianchi ◽  
Dino Collavo ◽  
Attilio Cecchetto
Keyword(s):  
Muscle Fibers ◽  
Cell Types ◽  
Neoplastic Tissue ◽  
Virus Particles ◽  
Induced Tumors ◽  
Granulocyte Infiltration ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Type C ◽  
Murine Sarcoma

Various growth phases of Moloney murine sarcoma virus (M-MSV) induced tumors in suckling and young adult BALB/c mice have been studied by light and electron microscopy. In the early phase (3-6 days following M-MSV), observations at the injection site of the thigh muscles consisted of endo- and perimysial edema, «activated» muscle satellite cells, endothelial cells and fibroblasts, scattered type C virus particles within the muscle fibers, muscle fibers and endomysial cells undergoing necrosis and macrophage and granulocyte infiltration. During the overt tumor phase (6-12 days following M-MSV), observation of neoplastic tissue disclosed proliferation of several cell types (endothelial, periosteal, fibroblasts, etc.), poorly differentiated myoblasts along with atypical rhabdo-myoblast-like cells and sarcolytes, type C virus budding from muscle fiber and myoblast plasma membrane, and intense degenerative and regenerative changes in the muscle fibers together with more profuse granulocyte infiltration. The regressive phase (13-21 days following M-MSV) presented reduced cellularity of the neoplastic tissue, a decrease in blast cells, diminishing granulocyte infiltration with contemporaneous appearance of prominent lymphocyte foci and gradual disappearance of virus particles. Although many cell types of mesenchymal origin proliferate following M-MSV infection, the above morphological findings indicate that striated muscle is a preferential site for virus replication and transformation. Furthermore, the peculiar virus cell relationships leading to cell lysis and continuous recruitment of newly infected cells have been widely documented. In the light of these findings it is suggested that, besides the host immune control of virus spread and tumor cell multiplication, the non clonal growth pattern of M-MSV induced tumors is a crucial factor in determining the spontaneous regression which occurs with high frequency in this experimental system.

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