Identification of Major Alkaloids in Rat Urine by HPLC/DAD/ESI-MS/MS Method Following Oral Administration of CortexPhellodendriDecoction

2009 ◽  
Vol 92 (2) ◽  
pp. 379-398 ◽  
Author(s):  
Chun-Hui Ma ◽  
Zhi-Xiong Li ◽  
Long-Xing Wang ◽  
Yi-Hong Tang ◽  
Hong-Bin Xiao ◽  
...  
Keyword(s):  
Oral Administration ◽  
Rat Urine ◽  
Esi Ms

Influence of processing on pharmacokinetic of typical constituents in radix polygoni multiflori after oral administration by LC–ESI–MS/MS

2013 ◽  
Vol 148 (1) ◽  
pp. 246-253 ◽  
Author(s):  
Ling Zhang ◽  
Wen-fang Ma ◽  
Jin Li ◽  
Jun He ◽  
Peng Zhang ◽  
...  
Keyword(s):  
Oral Administration ◽  
Esi Ms

scholarly journals Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jinglong Wang ◽  
Dandan Zheng ◽  
Nan Xu ◽  
Chao Zhang ◽  
Yingzi Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Oral Administration ◽  
Mass Spectrometry Data ◽  
Mass Spectrometry Analysis ◽  
Original Form ◽  
Bioactive Constituents ◽  
Serum Samples ◽  
Rat Serum ◽  
Esi Ms

AbstractTo realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction.

cAMP content in rat urine following 10 and 20 days oral administration of a calcium-binding ion exchanger

1979 ◽  
Vol 7 (4) ◽  
Author(s):  
R. Markstein ◽  
V. Hagmaier ◽  
M. H�berle ◽  
B. Kremer ◽  
G. Rutishauser
Keyword(s):  
Oral Administration ◽  
Calcium Binding ◽  
Ion Exchanger ◽  
Rat Urine ◽  
Camp Content

scholarly journals Rapid Identification of the Multiple Absorbed Bioactive Components and Metabolites in Rat Serum after Oral Administration of Wu-Jia Sheng-Hua Capsule by UPLC-ESI-MS

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Fang Geng ◽  
Fashan Wang ◽  
Taobo Zou ◽  
Kuiyuan Zhu ◽  
Wei Ma ◽  
...  
Keyword(s):  
Oral Administration ◽  
Ammonium Acetate ◽  
Gradient Elution ◽  
Rapid Identification ◽  
Ionization Mass ◽  
Pharmacological Effects ◽  
Bioactive Components ◽  
Rat Serum ◽  
Esi Ms ◽  
Acquity Uplc

To identify the compounds absorbed in rat serum after the oral administration of Wu-Jia Sheng-Hua (WJSH) capsule, a traditional Chinese medicine (TCM) compound prescription, an ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC/ESI-MS) method, was established. The chromatographic separation of the absorbed compounds and metabolites was achieved with an ACQUITY UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm) under a gradient elution. The mobile phase was composed of acetonitrile and water buffered with ammonium acetate (10 mM) and formic acid (0.1%, V/V). Twelve absorbed compounds and four metabolites were found. Seven of the absorbed compounds were identified by ESI-MS. The identification of absorbed compounds might be helpful for the better understanding of the mechanisms underlying the pharmacological effects of WJSH capsule.

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