scholarly journals Construction of the Fingerprints of Ginseng Stem and Leaf Saponin Reference Substances and Spiked Plasma Sample by LC-ESI/MS and Its Application to Analyzing the Compounds Absorbed into Blood after Oral Administration of Ginseng Stem and Leaf Saponin in Rat

2007 ◽  
Vol 30 (9) ◽  
pp. 1657-1662 ◽  
Author(s):  
Bei Yan ◽  
Guangji Wang ◽  
Jiye A ◽  
Lin Xie ◽  
Haiping Hao ◽  
...  
Keyword(s):  
Oral Administration ◽  
Plasma Sample ◽  
Esi Ms ◽  
Spiked Plasma ◽  
Spiked Plasma Sample

AQbD Oriented New LC-ESI/MS Method for Quantification of Sirolimus in Drug and Spiked Plasma Sample

2018 ◽  
Vol 14 (5) ◽  
pp. 519-529
Author(s):  
Mani Sumithra ◽  
Velayutham Ravichandiran ◽  
Palani Shanmugasundarm
Keyword(s):  
Plasma Sample ◽  
Esi Ms ◽  
Spiked Plasma ◽  
Spiked Plasma Sample

Development and validation of an ultra-performance liquid chromatography method coupled with tandem mass spectrometry for determination of alizapride in human plasma

RSC Advances ◽  
2015 ◽  
Vol 5 (93) ◽  
pp. 76377-76382 ◽  
Author(s):  
H. E. Zaazaa ◽  
E. S. Elzanfaly ◽  
A. T. Soudi ◽  
M. Y. Salem
Keyword(s):  
Plasma Sample ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Plasma Samples ◽  
Chromatography Method ◽  
Spiked Plasma ◽  
Liquid Chromatography Method ◽  
Development And Validation ◽  
Spiked Plasma Sample

Representative (a) blank plasma samples, (b) spiked plasma sample at LLQQ (1 ng mL−1) with the IS, (c) spiked plasma sample at QCH (800 ng mL−1) with the IS and (d) real plasma sample at 10 minutes spiked with the IS.

Influence of processing on pharmacokinetic of typical constituents in radix polygoni multiflori after oral administration by LC–ESI–MS/MS

2013 ◽  
Vol 148 (1) ◽  
pp. 246-253 ◽  
Author(s):  
Ling Zhang ◽  
Wen-fang Ma ◽  
Jin Li ◽  
Jun He ◽  
Peng Zhang ◽  
...  
Keyword(s):  
Oral Administration ◽  
Esi Ms

scholarly journals Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jinglong Wang ◽  
Dandan Zheng ◽  
Nan Xu ◽  
Chao Zhang ◽  
Yingzi Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Oral Administration ◽  
Mass Spectrometry Data ◽  
Mass Spectrometry Analysis ◽  
Original Form ◽  
Bioactive Constituents ◽  
Serum Samples ◽  
Rat Serum ◽  
Esi Ms

AbstractTo realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction.

Identification of Major Alkaloids in Rat Urine by HPLC/DAD/ESI-MS/MS Method Following Oral Administration of CortexPhellodendriDecoction

2009 ◽  
Vol 92 (2) ◽  
pp. 379-398 ◽  
Author(s):  
Chun-Hui Ma ◽  
Zhi-Xiong Li ◽  
Long-Xing Wang ◽  
Yi-Hong Tang ◽  
Hong-Bin Xiao ◽  
...  
Keyword(s):  
Oral Administration ◽  
Rat Urine ◽  
Esi Ms

Quetiapine Cross-Reactivity with Plasma Tricyclic Antidepressant Immunoassays

2005 ◽  
Vol 39 (9) ◽  
pp. 1446-1449 ◽  
Author(s):  
E Martin Caravati ◽  
JoEtta M Juenke ◽  
Barbara I Crouch ◽  
Kathleen T Anderson
Keyword(s):  
Tricyclic Antidepressants ◽  
Antipsychotic Agent ◽  
Structural Similarity ◽  
Cross Reactivity ◽  
Tricyclic Antidepressant ◽  
Spiked Plasma ◽  
Screening Assays ◽  
Drug Free ◽  
Spiked Plasma Sample

BACKGROUND: Toxicology screens obtained on patients who have overdosed on drugs frequently include tricyclic antidepressants (TCAs) as part of the evaluation. Quetiapine is an antipsychotic agent with structural similarity to the TCAs. OBJECTIVE: To determine whether quetiapine may cross-react with plasma TCA immunoassays in vitro using commonly available autoanalyzers. METHODS: Quetiapine stock solution was added to 9 separate samples of pooled drug-free human plasma to produce concentrations ranging from 1 to 640 ng/mL that were verified by gas chromatography. No quetiapine metabolites were present. Each spiked plasma sample was tested in a blinded fashion using the Abbott Tricyclic Antidepressant TDx Assay on the TDxFLx autoanalyzer in 2 separate laboratories, the Syva Emit tox Serum Tricyclic Antidepressant Assay on the AU400 autoanalyzer and the S TAD Serum Tricyclic Antidepressant Screen on the ACA-Star 300 autoanalyzer. The TDx assay is quantitative, while Emit and S TAD are qualitative screening assays with a threshold of 300 ng/mL for TCA positivity. The outcome of interest was a positive TCA result. RESULTS: The quantitative assay showed concentration-related TCA cross-reactivity beginning at quetiapine concentrations of 5 ng/mL. The 640-ng/mL spiked sample produced TCA results of 379 and 385 ng/mL in labs 1 and 2, respectively. The qualitative assays were screened as TCA positive at quetiapine concentrations of 160 and 320 ng/mL for the S TAD and Emit assays, respectively. CONCLUSIONS: Quetiapine cross-reacts with quantitative and qualitative plasma TCA immunoassays in a concentration-dependent fashion. Therapeutic use or overdose of quetiapine may result in a false-positive TCA immunoassay result.

scholarly journals Rapid Identification of the Multiple Absorbed Bioactive Components and Metabolites in Rat Serum after Oral Administration of Wu-Jia Sheng-Hua Capsule by UPLC-ESI-MS

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Fang Geng ◽  
Fashan Wang ◽  
Taobo Zou ◽  
Kuiyuan Zhu ◽  
Wei Ma ◽  
...  
Keyword(s):  
Oral Administration ◽  
Ammonium Acetate ◽  
Gradient Elution ◽  
Rapid Identification ◽  
Ionization Mass ◽  
Pharmacological Effects ◽  
Bioactive Components ◽  
Rat Serum ◽  
Esi Ms ◽  
Acquity Uplc

To identify the compounds absorbed in rat serum after the oral administration of Wu-Jia Sheng-Hua (WJSH) capsule, a traditional Chinese medicine (TCM) compound prescription, an ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC/ESI-MS) method, was established. The chromatographic separation of the absorbed compounds and metabolites was achieved with an ACQUITY UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm) under a gradient elution. The mobile phase was composed of acetonitrile and water buffered with ammonium acetate (10 mM) and formic acid (0.1%, V/V). Twelve absorbed compounds and four metabolites were found. Seven of the absorbed compounds were identified by ESI-MS. The identification of absorbed compounds might be helpful for the better understanding of the mechanisms underlying the pharmacological effects of WJSH capsule.

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