cAMP content in rat urine following 10 and 20 days oral administration of a calcium-binding ion exchanger

1979 ◽  
Vol 7 (4) ◽  
Author(s):  
R. Markstein ◽  
V. Hagmaier ◽  
M. H�berle ◽  
B. Kremer ◽  
G. Rutishauser
Keyword(s):  
Oral Administration ◽  
Calcium Binding ◽  
Ion Exchanger ◽  
Rat Urine ◽  
Camp Content

Identification of Major Alkaloids in Rat Urine by HPLC/DAD/ESI-MS/MS Method Following Oral Administration of CortexPhellodendriDecoction

2009 ◽  
Vol 92 (2) ◽  
pp. 379-398 ◽  
Author(s):  
Chun-Hui Ma ◽  
Zhi-Xiong Li ◽  
Long-Xing Wang ◽  
Yi-Hong Tang ◽  
Hong-Bin Xiao ◽  
...  
Keyword(s):  
Oral Administration ◽  
Rat Urine ◽  
Esi Ms

Rapid Profiling and Identification of Vitexin Metabolites in Rat Urine, Plasma and Faeces after Oral Administration Using a UHPLC-Q-Exactive Orbitrap Mass Spectrometer Coupled With Multiple Data-mining Methods

2021 ◽  
Vol 21 ◽  
Author(s):  
Pingping Dong ◽  
Lei Shi ◽  
Shaoping Wang ◽  
Shan Jiang ◽  
Haoran Li ◽  
...  
Keyword(s):  
Data Mining ◽  
Oral Administration ◽  
Mass Spectrometer ◽  
Flavonoid Compound ◽  
Ring Cleavage ◽  
Rat Urine ◽  
Multiple Data ◽  
Orbitrap Mass Spectrometer ◽  
Q Exactive ◽  
Mining Methods

Background: Vitexin is a natural flavonoid compound with multiple pharmacological activities and is extracted from the leaves and seeds of Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz. However, the metabolite characterization of this component remains insufficient. Objective: To establish a rapid profiling and identification method for vitexin metabolites in rat urine, plasma and faeces after oral administration using a UHPLC-Q-Exactive orbitrap mass spectrometer coupled with multiple data-mining methods. Methods: In this study, a simple and rapid systematic strategy for the detection and identification of constituents was proposed based on UHPLC-Q-Exactive Orbitrap mass spectrometry in parallel reaction monitoring mode combining diagnostic fragment ion filtering techniques. Results: A total of 49 metabolites were fully or partially characterized based on their accurate mass, characteristic fragment ions, retention times, corresponding ClogP values, and so on. It is obvious that C-glycosyl flavonoids often display an [M+H-120]+ ion that represents the loss of C4H8O4. As a result, these metabolites were presumed to be generated through glucuronidation, sulfation, deglucosylation, dehydrogenation, methylation, hydrogenation, hydroxylation, ring cleavage and their composite reactions. Moreover, the characteristic fragmentation pathways of flavonoids, chalcones, and dihydrochalcones were summarized for the subsequent metabolite identification. Conclusion: The current study provided an overall metabolic profile of vitexin, which will be of great help in predicting the in vivo pharmacokinetic profiles and understanding the action mechanism of this active ingredient.

Simultaneous Determination of Triterpenoidal Saponins in Rat Urine After Oral Administration of Pulsatilla chinensis Extract Using HPLC–MS

2015 ◽  
Vol 78 (3-4) ◽  
pp. 231-239 ◽  
Author(s):  
Miao Miao Jin ◽  
Geng Shen Song ◽  
Ying Feng Du ◽  
Liang Cao ◽  
Hui Jun Xu ◽  
...  
Keyword(s):  
Oral Administration ◽  
Simultaneous Determination ◽  
Rat Urine ◽  
Pulsatilla Chinensis

scholarly journals Comparative Study on Excretive Characterization of Main Components in Herb Pair Notoginseng-Safflower and Single Herbs by LC–MS/MS

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 241 ◽  
Author(s):  
Ying-Yuan Lu ◽  
Jin-Yang Song ◽  
Yan Li ◽  
Yu-Qing Meng ◽  
Ming-Bo Zhao ◽  
...  
Keyword(s):  
Oral Administration ◽  
Gradient Elution ◽  
Fecal Excretion ◽  
Bioactive Constituents ◽  
Rat Urine ◽  
Liquid Chromatography Tandem Mass ◽  
Main Components ◽  
Cumulative Excretion

The herbal medicine combination of notoginseng-safflower has been commonly used clinically for the prevention and treatment of cardiovascular diseases. A reliable liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed for simultaneous determination of six bioactive components (hydroxysafflor yellow A, notoginsenoide R1, ginsenoside Rb1, Re, Rd, and Rg1) in rat urine and feces after oral administration of notoginseng total saponins (NS), safflower total flavonoids (SF), and the combination of NS and SF (CNS). The chromatographic separation was achieved on a Waters HSS T3 column under gradient elution with acetonitrile and water containing formic acid as the mobile phase. The calibration curves were linear, with correlation coefficient (r) > 0.99 for six components. The intra- and interday precision (RSD) and accuracy (RE) of QC samples were within −14.9% and 14.9%, respectively. The method was successfully applied to study of the urinary and fecal excretion of six bioactive constituents following oral administration of NS, SF, and CNS in rats. Compared to the single herb, the cumulative excretion ratios of six constituents were decreased in the herbal combination. The study indicated that the combination of notoginseng and safflower could reduce the renal and fecal excretion of the major bioactive constituents and promote their absorption in rats.

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