Cytochrome P4501A induction potencies of polycyclic aromatic hydrocarbons in a fish hepatoma cell line: Demonstration of additive interactions

2000 ◽  
Vol 19 (8) ◽  
pp. 2047-2058 ◽  
Author(s):  
Karl Fent ◽  
Roger Bätscher
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Cell Line ◽  
Aromatic Hydrocarbons ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Cytochrome P4501a ◽  
Polycyclic Aromatic ◽  
Additive Interactions

Use of the Rainbow Trout Hepatoma Cell Line, RTH-149, in a Cytotoxicity Assay

1989 ◽  
Vol 17 (2) ◽  
pp. 67-71
Author(s):  
Harvey Babich ◽  
Nieves Martin-Alguacil ◽  
Ellen Borenfreund
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Rainbow Trout ◽  
Cell Line ◽  
Aromatic Hydrocarbons ◽  
Hepatoma Cell ◽  
Neutral Red ◽  
Cytotoxicity Assay ◽  
Hepatoma Cell Line ◽  
Polycyclic Aromatic ◽  
Direct Acting

The rainbow trout hepatoma cell line, RTH-149, was evaluated for use as a bioindicator cell type in the neutral red cytotoxicity assay. The cells were exposed for six days to various polycyclic aromatic hydrocarbons, including chemicals that are direct-acting toxicants and chemicals that require enzymatic biotransformation to cytotoxic metabolites. Whereas benzo[a]pyrene was only slightly cytotoxic, its metabolites — (±)trans-7,8-diol-benzo[a]pyrene and 3-hydroxy-benzo[a]pyrene — were highly cytotoxic. 7,12-Dimethylbenz[a]anthracene was cytotoxic, but cytotoxicity did not occur with benzo[a]anthracene, benzo[b]fluoranthene and benzo[k]fluoranthene. This cell line appears to lack sufficient xenobiotic metabolising capacity to biotransform many of these polycyclic aromatic hydrocarbons to activated cytotoxic metabolites.

Smoke Chemistry, In Vitro Cytotoxicity, and Genotoxicity Demonstrates Enhanced Toxicity of Cigarillos Compared With Cigarettes

2020 ◽  
Author(s):  
Lynn Crosby ◽  
Berran Yucesoy ◽  
Carmine Leggett ◽  
Zheng Tu ◽  
Steven A Belinsky ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Cell Line ◽  
Aromatic Hydrocarbons ◽  
Mutation Frequency ◽  
International Organization ◽  
Tobacco Products ◽  
Cultured Cell Line ◽  
Polycyclic Aromatic ◽  
Tobacco Specific Nitrosamines

Abstract There has been limited toxicity testing of cigarillos, including comparison to cigarettes. This study compared the smoke chemistry and the cytotoxic and genotoxic potential of 10 conventional cigarettes and 10 cigarillos based on the greatest market share. Whole smoke and total particulate matter (TPM) were generated using the Canadian Intense and International Organization for Standardization puffing protocols. Tobacco-specific nitrosamines, carbonyls, and polycyclic aromatic hydrocarbons were measured using gas chromatography-mass spectrometry. TPM smoke extracts were used for the in vitro assays. Cytotoxicity was assessed in human bronchial epithelial continuously cultured cell line cells using the neutral red uptake assay. Genotoxic potential was assessed using the micronucleus (human lung adenocarcinoma continuously cultured cell line cells), Ames, and thymidine kinase assays. TPM from all cigarillos tested was more cytotoxic than cigarettes. Micronucleus formation was significantly greater for cigarillos compared with cigarettes at the highest dose of TPM, with or without rat liver S9 fraction. In the Ames test +S9, both tobacco products exhibited significant dose-dependent increases in mutation frequency, indicating metabolic activation is required for genotoxicity. In the thymidine kinase assay +S9, cigarillos showed a significantly enhanced mutation frequency although both tobacco products were positive. The levels of all measured polycyclic aromatic hydrocarbons, tobacco-specific nitrosamines, and carbonyls (except acrolein) were significantly greater in cigarillos than cigarettes. The Canadian Intense puffing protocol demonstrated increased smoke constituent levels compared with International Organization for Standardization. Even though the gas vapor phase was not tested, the results of this study showed that under the tested conditions the investigated cigarillos showed greater toxicity than comparator cigarettes. This study found that there is significantly greater toxicity in the tested U.S. marketed cigarillos than cigarettes for tobacco constituent levels, cytotoxicity, and genotoxicity. These findings are important for understanding the human health toxicity from the use of cigarillos relative to cigarettes and for building upon knowledge regarding harm from cigarillos to inform risk mitigation strategies.

Sign in / Sign up

Export Citation Format

Share Document